(Linear) (Six-base) MAPSORT of: KIBB9548.seq  Check: 8215  from: 1  to: 381



KIBB9548.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AfeI AGC'GCT

Cuts at:      0     209     381 
   Size:        209     172
 
AvaI C'yCGr_G

Cuts at:      0     203     381 
   Size:        203     178
 
BanI G'GyrC_C

Cuts at:      0     199     381 
   Size:        199     182
 
BanII G_rGCy'C

Cuts at:      0     189     368     381 
   Size:        189     179      13

  Fragments arranged by size: 

                 189     179      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     353     381 
   Size:        353      28
 
Bme1580I G_kGCm'C

Cuts at:      0     202     231     381 
   Size:        202      29     150

  Fragments arranged by size: 

                 202     150      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     360     381 
   Size:        360      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     268     381 
   Size:        196      72     113

  Fragments arranged by size: 

                 196     113      72
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     381 
   Size:        274     107
 
BsaBI GATnn'nnATC

Cuts at:      0     307     381 
   Size:        307      74
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     381 
   Size:         64     317
 
BseYI C'CCAG_C

Cuts at:      0     195     381 
   Size:        195     186
 
BsiEI CG_ry'CG

Cuts at:      0     133     381 
   Size:        133     248
 
BsiHKAI G_wGCw'C

Cuts at:      0     368     381 
   Size:        368      13
 
BsmI GAATG_Cn'

Cuts at:      0      84     381 
   Size:         84     297
 
Bsp1286I G_dGCh'C

Cuts at:      0     189     202     231     368     381 
   Size:        189      13      29     137      13

  Fragments arranged by size: 

                 189     137      29      13      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     335     381 
   Size:        335      46
 
BsrDI GCAATG_nn'

Cuts at:      0     272     381 
   Size:        272     109
 
BstYI r'GATC_y

Cuts at:      0     110     261     302     381 
   Size:        110     151      41      79

  Fragments arranged by size: 

                 151     110      79      41
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     235     381 
   Size:        235     146
 
EarI CTCTTCn'nnn_

Cuts at:      0      80     381 
   Size:         80     301
 
EcoICRI GAG'CTC

Cuts at:      0     366     381 
   Size:        366      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     268     381 
   Size:        196      72     113

  Fragments arranged by size: 

                 196     113      72
 
HaeII r_GCGC'y

Cuts at:      0     211     381 
   Size:        211     170
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     281     381 
   Size:        249      32     100

  Fragments arranged by size: 

                 249     100      32
 
MslI CAynn'nnrTG

Cuts at:      0     277     381 
   Size:        277     104
 
SacI G_AGCT'C

Cuts at:      0     368     381 
   Size:        368      13
 
SmlI C'TyrA_G

Cuts at:      0     289     381 
   Size:        289      92
 

 Enzymes that do cut:

     AfeI     AvaI     BanI    BanII     BbsI Bme1580I     BmrI     BpmI
    BpuEI    BsaBI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BstYI    BtgZI     EarI  EcoICRI  Eco57MI    HaeII
    Hin4I     MslI     SacI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI     BsgI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI