Restriction map for
pF1KB9549
(Linear) (Six-base) MAPSORT of: KIBB9549.seq Check: 5637 from: 1 to: 1404
KIBB9549.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1241 1404
Size: 1241 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1404
Size: 92 1312
AflIII A'CryG_T
Cuts at: 0 350 1215 1397 1404
Size: 350 865 182 7
Fragments arranged by size:
865 350 182 7
AleI CACnn'nnGTG
Cuts at: 0 353 838 1404
Size: 353 485 566
Fragments arranged by size:
566 485 353
ApaLI G'TGCA_C
Cuts at: 0 831 1404
Size: 831 573
ApoI r'AATT_y
Cuts at: 0 560 865 1024 1404
Size: 560 305 159 380
Fragments arranged by size:
560 380 305 159
AvaI C'yCGr_G
Cuts at: 0 40 674 1404
Size: 40 634 730
Fragments arranged by size:
730 634 40
BanII G_rGCy'C
Cuts at: 0 64 232 860 1404
Size: 64 168 628 544
Fragments arranged by size:
628 544 168 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 760 956 1351 1404
Size: 760 196 395 53
Fragments arranged by size:
760 395 196 53
BglII A'GATC_T
Cuts at: 0 532 603 1385 1404
Size: 532 71 782 19
Fragments arranged by size:
782 532 71 19
Bme1580I G_kGCm'C
Cuts at: 0 835 1404
Size: 835 569
BmgBI CAC'GTC
Cuts at: 0 836 1404
Size: 836 568
BmrI ACTGGGnnnn_n'
Cuts at: 0 275 1404
Size: 275 1129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1404
Size: 379 1025
BsaI GGTCTCn'nnnn_
Cuts at: 0 838 1404
Size: 838 566
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 724 754 1404
Size: 724 30 650
Fragments arranged by size:
724 650 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 1404
Size: 225 1179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 1404
Size: 1351 53
BsiEI CG_ry'CG
Cuts at: 0 420 1404
Size: 420 984
BsiHKAI G_wGCw'C
Cuts at: 0 835 860 1223 1404
Size: 835 25 363 181
Fragments arranged by size:
835 363 181 25
BsmI GAATG_Cn'
Cuts at: 0 530 1404
Size: 530 874
Bsp1286I G_dGCh'C
Cuts at: 0 64 232 835 860 1223 1404
Size: 64 168 603 25 363 181
Fragments arranged by size:
603 363 181 168 64 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1241 1404
Size: 1241 163
BsrDI GCAATG_nn'
Cuts at: 0 919 1404
Size: 919 485
BsrFI r'CCGG_y
Cuts at: 0 46 1404
Size: 46 1358
BsrGI T'GTAC_A
Cuts at: 0 1212 1404
Size: 1212 192
BssSI C'ACGA_G
Cuts at: 0 1019 1404
Size: 1019 385
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1329 1404
Size: 1329 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 774 1404
Size: 774 630
BstYI r'GATC_y
Cuts at: 0 431 532 603 1385 1404
Size: 431 101 71 782 19
Fragments arranged by size:
782 431 101 71 19
Bsu36I CC'TnA_GG
Cuts at: 0 327 1039 1404
Size: 327 712 365
Fragments arranged by size:
712 365 327
BtsI GCAGTG_nn'
Cuts at: 0 819 1404
Size: 819 585
EaeI y'GGCC_r
Cuts at: 0 617 764 776 1404
Size: 617 147 12 628
Fragments arranged by size:
628 617 147 12
EarI CTCTTCn'nnn_
Cuts at: 0 68 1270 1404
Size: 68 1202 134
Fragments arranged by size:
1202 134 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 817 1404
Size: 817 587
EcoICRI GAG'CTC
Cuts at: 0 858 1404
Size: 858 546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1404
Size: 92 1312
EcoRI G'AATT_C
Cuts at: 0 865 1024 1404
Size: 865 159 380
Fragments arranged by size:
865 380 159
EcoRV GAT'ATC
Cuts at: 0 1059 1122 1257 1404
Size: 1059 63 135 147
Fragments arranged by size:
1059 147 135 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 1404
Size: 390 1014
FspI TGC'GCA
Cuts at: 0 1322 1404
Size: 1322 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 595 627 1404
Size: 310 32 253 32 777
Fragments arranged by size:
777 310 253 32 32
HindIII A'AGCT_T
Cuts at: 0 141 1404
Size: 141 1263
MluI A'CGCG_T
Cuts at: 0 350 1404
Size: 350 1054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 1404
Size: 1334 70
MscI TGG'CCA
Cuts at: 0 619 766 778 1404
Size: 619 147 12 626
Fragments arranged by size:
626 619 147 12
MslI CAynn'nnrTG
Cuts at: 0 353 838 1404
Size: 353 485 566
Fragments arranged by size:
566 485 353
MspA1I CmG'CkG
Cuts at: 0 425 1305 1326 1404
Size: 425 880 21 78
Fragments arranged by size:
880 425 78 21
NaeI GCC'GGC
Cuts at: 0 48 1404
Size: 48 1356
NgoMIV G'CCGG_C
Cuts at: 0 46 1404
Size: 46 1358
NspI r_CATG'y
Cuts at: 0 1219 1321 1401 1404
Size: 1219 102 80 3
Fragments arranged by size:
1219 102 80 3
PciI A'CATG_T
Cuts at: 0 1215 1397 1404
Size: 1215 182 7
Fragments arranged by size:
1215 182 7
PsiI TTA'TAA
Cuts at: 0 1015 1404
Size: 1015 389
PspXI vC'TCGA_Gb
Cuts at: 0 40 1404
Size: 40 1364
PstI C_TGCA'G
Cuts at: 0 603 1238 1382 1404
Size: 603 635 144 22
Fragments arranged by size:
635 603 144 22
PvuII CAG'CTG
Cuts at: 0 425 1305 1326 1404
Size: 425 880 21 78
Fragments arranged by size:
880 425 78 21
SacI G_AGCT'C
Cuts at: 0 860 1404
Size: 860 544
SfcI C'TryA_G
Cuts at: 0 599 1234 1378 1389 1404
Size: 599 635 144 11 15
Fragments arranged by size:
635 599 144 15 11
SmaI CCC'GGG
Cuts at: 0 676 1404
Size: 676 728
SmlI C'TyrA_G
Cuts at: 0 40 394 1404
Size: 40 354 1010
Fragments arranged by size:
1010 354 40
SphI G_CATG'C
Cuts at: 0 1321 1404
Size: 1321 83
StuI AGG'CCT
Cuts at: 0 325 637 1404
Size: 325 312 767
Fragments arranged by size:
767 325 312
StyI C'CwwG_G
Cuts at: 0 872 1404
Size: 872 532
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 1404
Size: 186 1218
TatI w'GTAC_w
Cuts at: 0 1212 1404
Size: 1212 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 724 756 1404
Size: 724 32 648
Fragments arranged by size:
724 648 32
Tth111I GACn'n_nGTC
Cuts at: 0 1350 1404
Size: 1350 54
XbaI T'CTAG_A
Cuts at: 0 54 1404
Size: 54 1350
XhoI C'TCGA_G
Cuts at: 0 40 1404
Size: 40 1364
XmaI C'CCGG_G
Cuts at: 0 674 1404
Size: 674 730
XmnI GAAnn'nnTTC
Cuts at: 0 649 1048 1404
Size: 649 399 356
Fragments arranged by size:
649 399 356
Enzymes that do cut:
AarI AcuI AflIII AleI ApaLI ApoI AvaI BanII
BbsI BglII Bme1580I BmgBI BmrI BpuEI BsaI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI Hin4I HindIII MluI MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PciI PsiI PspXI PstI PvuII
SacI SfcI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmtI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BseYI BsiWI BsmBI BspEI BspHI
BsrBI BssHII BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoO109I FseI
FspAI HaeII HincII HpaI KasI KpnI MfeI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XcmI ZraI