Restriction map for
pF1KB9553
(Linear) (Six-base) MAPSORT of: KIBB9553.seq Check: 5724 from: 1 to: 783
KIBB9553.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 513 783
Size: 513 270
AatII G_ACGT'C
Cuts at: 0 753 783
Size: 753 30
AccI GT'mk_AC
Cuts at: 0 697 783
Size: 697 86
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 571 783
Size: 452 119 212
Fragments arranged by size:
452 212 119
AfeI AGC'GCT
Cuts at: 0 406 783
Size: 406 377
AlwNI CAG_nnn'CTG
Cuts at: 0 105 600 669 783
Size: 105 495 69 114
Fragments arranged by size:
495 114 105 69
ApaLI G'TGCA_C
Cuts at: 0 509 783
Size: 509 274
AseI AT'TA_AT
Cuts at: 0 775 783
Size: 775 8
AvaI C'yCGr_G
Cuts at: 0 205 783
Size: 205 578
BanI G'GyrC_C
Cuts at: 0 117 783
Size: 117 666
BanII G_rGCy'C
Cuts at: 0 90 205 688 783
Size: 90 115 483 95
Fragments arranged by size:
483 115 95 90
BbvCI CC'TCA_GC
Cuts at: 0 220 783
Size: 220 563
BglI GCCn_nnn'nGGC
Cuts at: 0 100 783
Size: 100 683
Bme1580I G_kGCm'C
Cuts at: 0 513 783
Size: 513 270
BmrI ACTGGGnnnn_n'
Cuts at: 0 528 630 706 783
Size: 528 102 76 77
Fragments arranged by size:
528 102 77 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 699 783
Size: 532 167 84
Fragments arranged by size:
532 167 84
Bpu10I CC'TnA_GC
Cuts at: 0 199 220 783
Size: 199 21 563
Fragments arranged by size:
563 199 21
BsaAI yAC'GTr
Cuts at: 0 508 783
Size: 508 275
BsaHI Gr'CG_yC
Cuts at: 0 750 783
Size: 750 33
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 237 451 696 783
Size: 223 14 214 245 87
Fragments arranged by size:
245 223 214 87 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 783
Size: 547 236
BsiEI CG_ry'CG
Cuts at: 0 32 95 783
Size: 32 63 688
Fragments arranged by size:
688 63 32
BsiHKAI G_wGCw'C
Cuts at: 0 513 688 783
Size: 513 175 95
Fragments arranged by size:
513 175 95
BsmI GAATG_Cn'
Cuts at: 0 616 783
Size: 616 167
Bsp1286I G_dGCh'C
Cuts at: 0 90 205 513 688 783
Size: 90 115 308 175 95
Fragments arranged by size:
308 175 115 95 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 513 783
Size: 513 270
BsrBI CCG'CTC
Cuts at: 0 111 155 783
Size: 111 44 628
Fragments arranged by size:
628 111 44
BsrFI r'CCGG_y
Cuts at: 0 94 783
Size: 94 689
BssHII G'CGCG_C
Cuts at: 0 172 783
Size: 172 611
BstAPI GCAn_nnn'nTGC
Cuts at: 0 525 783
Size: 525 258
BtgI C'CryG_G
Cuts at: 0 562 783
Size: 562 221
EaeI y'GGCC_r
Cuts at: 0 29 92 96 473 783
Size: 29 63 4 377 310
Fragments arranged by size:
377 310 63 29 4
EagI C'GGCC_G
Cuts at: 0 29 92 783
Size: 29 63 691
Fragments arranged by size:
691 63 29
EarI CTCTTCn'nnn_
Cuts at: 0 314 428 590 783
Size: 314 114 162 193
Fragments arranged by size:
314 193 162 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 457 783
Size: 66 391 326
Fragments arranged by size:
391 326 66
EcoICRI GAG'CTC
Cuts at: 0 686 783
Size: 686 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 532 571 699 783
Size: 452 80 39 128 84
Fragments arranged by size:
452 128 84 80 39
EcoO109I rG'GnC_Cy
Cuts at: 0 38 102 708 783
Size: 38 64 606 75
Fragments arranged by size:
606 75 64 38
EcoRV GAT'ATC
Cuts at: 0 605 783
Size: 605 178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 70 783
Size: 70 713
FseI GG_CCGG'CC
Cuts at: 0 98 783
Size: 98 685
HaeII r_GCGC'y
Cuts at: 0 189 220 408 783
Size: 189 31 188 375
Fragments arranged by size:
375 189 188 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 173 205 582 614 783
Size: 173 32 377 32 169
Fragments arranged by size:
377 173 169 32 32
NaeI GCC'GGC
Cuts at: 0 96 783
Size: 96 687
NcoI C'CATG_G
Cuts at: 0 562 783
Size: 562 221
NgoMIV G'CCGG_C
Cuts at: 0 94 783
Size: 94 689
PpuMI rG'GwC_Cy
Cuts at: 0 38 708 783
Size: 38 670 75
Fragments arranged by size:
670 75 38
PstI C_TGCA'G
Cuts at: 0 521 783
Size: 521 262
SacI G_AGCT'C
Cuts at: 0 688 783
Size: 688 95
SanDI GG'GwC_CC
Cuts at: 0 38 783
Size: 38 745
SfcI C'TryA_G
Cuts at: 0 517 783
Size: 517 266
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 100 783
Size: 100 683
StyI C'CwwG_G
Cuts at: 0 449 562 783
Size: 449 113 221
Fragments arranged by size:
449 221 113
Tth111I GACn'n_nGTC
Cuts at: 0 636 783
Size: 636 147
ZraI GAC'GTC
Cuts at: 0 751 783
Size: 751 32
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AlwNI ApaLI AseI
AvaI BanI BanII BbvCI BglI Bme1580I BmrI BpmI
Bpu10I BsaAI BsaHI BseRI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BtgI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI
FseI HaeII Hin4I NaeI NcoI NgoMIV PpuMI PstI
SacI SanDI SfcI SfiI StyI Tth111I ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaI ApoI AscI AsiSI AvrII BaeI BamHI
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BpuEI BsaI BsaBI BsaWI BsaXI
BseYI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EcoNI EcoRI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
XbaI XcmI XhoI XmaI XmnI