Restriction map for
pF1KB9554
(Linear) (Six-base) MAPSORT of: KIBB9554.seq Check: 7830 from: 1 to: 510
KIBB9554.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AflIII A'CryG_T
Cuts at: 0 116 227 510
Size: 116 111 283
Fragments arranged by size:
283 116 111
AlwNI CAG_nnn'CTG
Cuts at: 0 252 444 510
Size: 252 192 66
Fragments arranged by size:
252 192 66
AvaI C'yCGr_G
Cuts at: 0 316 510
Size: 316 194
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3 36 510
Size: 3 33 474
Fragments arranged by size:
474 33 3
BanI G'GyrC_C
Cuts at: 0 462 510
Size: 462 48
BanII G_rGCy'C
Cuts at: 0 314 367 510
Size: 314 53 143
Fragments arranged by size:
314 143 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 399 510
Size: 399 111
BlpI GC'TnA_GC
Cuts at: 0 253 361 366 510
Size: 253 108 5 144
Fragments arranged by size:
253 144 108 5
Bme1580I G_kGCm'C
Cuts at: 0 465 510
Size: 465 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 361 510
Size: 349 12 149
Fragments arranged by size:
349 149 12
Bpu10I CC'TnA_GC
Cuts at: 0 308 510
Size: 308 202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 510
Size: 76 434
BsaI GGTCTCn'nnnn_
Cuts at: 0 233 510
Size: 233 277
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 510
Size: 190 30 290
Fragments arranged by size:
290 190 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 286 510
Size: 283 3 224
Fragments arranged by size:
283 224 3
BsiEI CG_ry'CG
Cuts at: 0 33 510
Size: 33 477
BsiHKAI G_wGCw'C
Cuts at: 0 314 367 510
Size: 314 53 143
Fragments arranged by size:
314 143 53
Bsp1286I G_dGCh'C
Cuts at: 0 314 367 465 510
Size: 314 53 98 45
Fragments arranged by size:
314 98 53 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 510
Size: 251 259
BsrBI CCG'CTC
Cuts at: 0 35 510
Size: 35 475
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 510
Size: 144 366
BtgI C'CryG_G
Cuts at: 0 419 510
Size: 419 91
EaeI y'GGCC_r
Cuts at: 0 427 510
Size: 427 83
EarI CTCTTCn'nnn_
Cuts at: 0 340 510
Size: 340 170
EcoICRI GAG'CTC
Cuts at: 0 312 365 510
Size: 312 53 145
Fragments arranged by size:
312 145 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 361 510
Size: 349 12 149
Fragments arranged by size:
349 149 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 30 510
Size: 30 480
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 510
Size: 190 32 288
Fragments arranged by size:
288 190 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 510
Size: 233 277
MscI TGG'CCA
Cuts at: 0 429 510
Size: 429 81
MslI CAynn'nnrTG
Cuts at: 0 262 510
Size: 262 248
MspA1I CmG'CkG
Cuts at: 0 133 510
Size: 133 377
NspI r_CATG'y
Cuts at: 0 120 231 510
Size: 120 111 279
Fragments arranged by size:
279 120 111
PciI A'CATG_T
Cuts at: 0 116 227 510
Size: 116 111 283
Fragments arranged by size:
283 116 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 290 510
Size: 290 220
PfoI T'CCnGG_A
Cuts at: 0 41 510
Size: 41 469
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 103 135 510
Size: 103 32 375
Fragments arranged by size:
375 103 32
PstI C_TGCA'G
Cuts at: 0 86 172 217 248 491 510
Size: 86 86 45 31 243 19
Fragments arranged by size:
243 86 86 45 31 19
PvuII CAG'CTG
Cuts at: 0 133 510
Size: 133 377
SacI G_AGCT'C
Cuts at: 0 314 367 510
Size: 314 53 143
Fragments arranged by size:
314 143 53
SbfI CC_TGCA'GG
Cuts at: 0 172 248 510
Size: 172 76 262
Fragments arranged by size:
262 172 76
SfcI C'TryA_G
Cuts at: 0 82 168 213 244 487 510
Size: 82 86 45 31 243 23
Fragments arranged by size:
243 86 82 45 31 23
SmlI C'TyrA_G
Cuts at: 0 91 510
Size: 91 419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 199 476 510
Size: 199 277 34
Fragments arranged by size:
277 199 34
Enzymes that do cut:
AflIII AlwNI AvaI BaeI BanI BanII BbsI BlpI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BstXI BtgI EaeI EarI
EcoICRI Eco57MI FalI Hin4I MmeI MscI MslI MspA1I
NspI PciI PflMI PfoI PsrI PstI PvuII SacI
SbfI SfcI SmlI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI