Restriction map for
pF1KB9556
(Linear) (Six-base) MAPSORT of: KIBB9556.seq Check: 2995 from: 1 to: 2613
KIBB9556.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2421 2613
Size: 2421 192
AatII G_ACGT'C
Cuts at: 0 2444 2613
Size: 2444 169
AccI GT'mk_AC
Cuts at: 0 1610 2042 2613
Size: 1610 432 571
Fragments arranged by size:
1610 571 432
AflII C'TTAA_G
Cuts at: 0 2121 2613
Size: 2121 492
AflIII A'CryG_T
Cuts at: 0 2460 2545 2613
Size: 2460 85 68
Fragments arranged by size:
2460 85 68
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 626 658 1000 1032 2613
Size: 626 32 342 32 1581
Fragments arranged by size:
1581 626 342 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 87 2613
Size: 87 2526
ApaI G_GGCC'C
Cuts at: 0 617 2613
Size: 617 1996
ApoI r'AATT_y
Cuts at: 0 1398 1921 2613
Size: 1398 523 692
Fragments arranged by size:
1398 692 523
AvaI C'yCGr_G
Cuts at: 0 472 682 2613
Size: 472 210 1931
Fragments arranged by size:
1931 472 210
AvrII C'CTAG_G
Cuts at: 0 852 2613
Size: 852 1761
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1375 1408 2613
Size: 1375 33 1205
Fragments arranged by size:
1375 1205 33
BanI G'GyrC_C
Cuts at: 0 201 253 357 507 1439 1867 2613
Size: 201 52 104 150 932 428 746
Fragments arranged by size:
932 746 428 201 150 104 52
BanII G_rGCy'C
Cuts at: 0 127 241 605 617 680 812 1193 2218
Size: 127 114 364 12 63 132 381 1025
Cuts at: 2218 2313 2613
Size: 95 300
Fragments arranged by size:
1025 381 364 300 132 127 114 95
63 12
BbeI G_GCGC'C
Cuts at: 0 257 1871 2613
Size: 257 1614 742
Fragments arranged by size:
1614 742 257
BbsI GAAGACnn'nnnn_
Cuts at: 0 1052 1697 2006 2613
Size: 1052 645 309 607
Fragments arranged by size:
1052 645 607 309
BbvCI CC'TCA_GC
Cuts at: 0 71 2613
Size: 71 2542
BciVI GTATCCnnnnn_n'
Cuts at: 0 1761 2613
Size: 1761 852
BglII A'GATC_T
Cuts at: 0 1376 2613
Size: 1376 1237
BlpI GC'TnA_GC
Cuts at: 0 2405 2613
Size: 2405 208
Bme1580I G_kGCm'C
Cuts at: 0 206 617 2613
Size: 206 411 1996
Fragments arranged by size:
1996 411 206
BmgBI CAC'GTC
Cuts at: 0 903 944 2613
Size: 903 41 1669
Fragments arranged by size:
1669 903 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 105 497 2613
Size: 105 392 2116
Fragments arranged by size:
2116 392 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 190 2613
Size: 190 2423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2159 2613
Size: 2159 454
Bpu10I CC'TnA_GC
Cuts at: 0 71 2613
Size: 71 2542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 2001 2613
Size: 811 1190 612
Fragments arranged by size:
1190 811 612
BsaI GGTCTCn'nnnn_
Cuts at: 0 923 2596 2613
Size: 923 1673 17
Fragments arranged by size:
1673 923 17
BsaHI Gr'CG_yC
Cuts at: 0 254 1868 2441 2613
Size: 254 1614 573 172
Fragments arranged by size:
1614 573 254 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 329 359 626 632 656 662 2613
Size: 329 30 267 6 24 6 1951
Fragments arranged by size:
1951 329 267 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 305 490 670 814 895 1941 2613
Size: 305 185 180 144 81 1046 672
Fragments arranged by size:
1046 672 305 185 180 144 81
BseYI C'CCAG_C
Cuts at: 0 257 381 1019 1913 2613
Size: 257 124 638 894 700
Fragments arranged by size:
894 700 638 257 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 1581 2613
Size: 450 1131 1032
Fragments arranged by size:
1131 1032 450
BsiEI CG_ry'CG
Cuts at: 0 64 576 2613
Size: 64 512 2037
Fragments arranged by size:
2037 512 64
BsiHKAI G_wGCw'C
Cuts at: 0 680 2069 2218 2613
Size: 680 1389 149 395
Fragments arranged by size:
1389 680 395 149
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2287 2613
Size: 2287 326
Bsp1286I G_dGCh'C
Cuts at: 0 127 206 241 605 617 680 812 1193
Size: 127 79 35 364 12 63 132 381
Cuts at: 1193 2069 2218 2313 2613
Size: 876 149 95 300
Fragments arranged by size:
876 381 364 300 149 132 127 95
79 63 35 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2421 2613
Size: 2421 192
BsrBI CCG'CTC
Cuts at: 0 268 2613
Size: 268 2345
BsrDI GCAATG_nn'
Cuts at: 0 1866 2613
Size: 1866 747
BsrFI r'CCGG_y
Cuts at: 0 423 2613
Size: 423 2190
BsrGI T'GTAC_A
Cuts at: 0 1898 2613
Size: 1898 715
BstEII G'GTnAC_C
Cuts at: 0 396 1743 2613
Size: 396 1347 870
Fragments arranged by size:
1347 870 396
BstYI r'GATC_y
Cuts at: 0 1243 1376 2613
Size: 1243 133 1237
Fragments arranged by size:
1243 1237 133
Bsu36I CC'TnA_GG
Cuts at: 0 1790 2613
Size: 1790 823
BtgI C'CryG_G
Cuts at: 0 439 695 2613
Size: 439 256 1918
Fragments arranged by size:
1918 439 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2483 2613
Size: 2483 130
BtsI GCAGTG_nn'
Cuts at: 0 518 965 2613
Size: 518 447 1648
Fragments arranged by size:
1648 518 447
DraI TTT'AAA
Cuts at: 0 1395 1926 2613
Size: 1395 531 687
Fragments arranged by size:
1395 687 531
DrdI GACnn_nn'nnGTC
Cuts at: 0 866 2613
Size: 866 1747
EaeI y'GGCC_r
Cuts at: 0 272 573 2613
Size: 272 301 2040
Fragments arranged by size:
2040 301 272
EagI C'GGCC_G
Cuts at: 0 573 2613
Size: 573 2040
EarI CTCTTCn'nnn_
Cuts at: 0 751 1175 1959 2080 2153 2613
Size: 751 424 784 121 73 460
Fragments arranged by size:
784 751 460 424 121 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 69 2613
Size: 69 2544
EcoICRI GAG'CTC
Cuts at: 0 678 2216 2613
Size: 678 1538 397
Fragments arranged by size:
1538 678 397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2159 2613
Size: 2159 454
EcoNI CCTnn'n_nnAGG
Cuts at: 0 557 2613
Size: 557 2056
EcoO109I rG'GnC_Cy
Cuts at: 0 107 480 588 614 849 859 1158 2613
Size: 107 373 108 26 235 10 299 1455
Fragments arranged by size:
1455 373 299 235 108 107 26 10
FspI TGC'GCA
Cuts at: 0 835 2613
Size: 835 1778
HaeII r_GCGC'y
Cuts at: 0 257 1871 2613
Size: 257 1614 742
Fragments arranged by size:
1614 742 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1428 1460 2350 2382 2407 2439
Size: 1168 32 228 32 890 32 25 32
Cuts at: 2439 2532 2564 2613
Size: 93 32 49
Fragments arranged by size:
1168 890 228 93 49 32 32 32
32 32 25
HincII GTy'rAC
Cuts at: 0 494 2613
Size: 494 2119
KasI G'GCGC_C
Cuts at: 0 253 1867 2613
Size: 253 1614 746
Fragments arranged by size:
1614 746 253
MfeI C'AATT_G
Cuts at: 0 2558 2613
Size: 2558 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 1481 2613
Size: 815 666 1132
Fragments arranged by size:
1132 815 666
MscI TGG'CCA
Cuts at: 0 274 2613
Size: 274 2339
MslI CAynn'nnrTG
Cuts at: 0 2261 2613
Size: 2261 352
MspA1I CmG'CkG
Cuts at: 0 261 441 1917 2315 2342 2613
Size: 261 180 1476 398 27 271
Fragments arranged by size:
1476 398 271 261 180 27
NarI GG'CG_CC
Cuts at: 0 254 1868 2613
Size: 254 1614 745
Fragments arranged by size:
1614 745 254
NdeI CA'TA_TG
Cuts at: 0 1584 2613
Size: 1584 1029
NspI r_CATG'y
Cuts at: 0 2549 2613
Size: 2549 64
PasI CC'CwG_GG
Cuts at: 0 864 913 2328 2613
Size: 864 49 1415 285
Fragments arranged by size:
1415 864 285 49
PciI A'CATG_T
Cuts at: 0 2545 2613
Size: 2545 68
PflMI CCAn_nnn'nTGG
Cuts at: 0 87 388 2613
Size: 87 301 2225
Fragments arranged by size:
2225 301 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 587 619 626 658 2613
Size: 587 32 7 32 1955
Fragments arranged by size:
1955 587 32 32 7
PpuMI rG'GwC_Cy
Cuts at: 0 480 588 859 2613
Size: 480 108 271 1754
Fragments arranged by size:
1754 480 271 108
PspOMI G'GGCC_C
Cuts at: 0 613 2613
Size: 613 2000
PstI C_TGCA'G
Cuts at: 0 2358 2432 2613
Size: 2358 74 181
Fragments arranged by size:
2358 181 74
PvuII CAG'CTG
Cuts at: 0 1917 2613
Size: 1917 696
SacI G_AGCT'C
Cuts at: 0 680 2218 2613
Size: 680 1538 395
Fragments arranged by size:
1538 680 395
SacII CC_GC'GG
Cuts at: 0 442 2613
Size: 442 2171
SapI GCTCTTCn'nnn_
Cuts at: 0 2153 2613
Size: 2153 460
SbfI CC_TGCA'GG
Cuts at: 0 2432 2613
Size: 2432 181
SfcI C'TryA_G
Cuts at: 0 1695 2354 2428 2613
Size: 1695 659 74 185
Fragments arranged by size:
1695 659 185 74
SfoI GGC'GCC
Cuts at: 0 255 1869 2613
Size: 255 1614 744
Fragments arranged by size:
1614 744 255
SmlI C'TyrA_G
Cuts at: 0 682 826 2016 2121 2613
Size: 682 144 1190 105 492
Fragments arranged by size:
1190 682 492 144 105
SspI AAT'ATT
Cuts at: 0 1458 2613
Size: 1458 1155
StyI C'CwwG_G
Cuts at: 0 852 2534 2613
Size: 852 1682 79
Fragments arranged by size:
1682 852 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 354 2375 2613
Size: 354 2021 238
Fragments arranged by size:
2021 354 238
TatI w'GTAC_w
Cuts at: 0 299 706 1898 2613
Size: 299 407 1192 715
Fragments arranged by size:
1192 715 407 299
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 329 361 1552 1584 2613
Size: 329 32 1191 32 1029
Fragments arranged by size:
1191 1029 329 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2443 2613
Size: 2443 170
XhoI C'TCGA_G
Cuts at: 0 682 2613
Size: 682 1931
ZraI GAC'GTC
Cuts at: 0 2442 2613
Size: 2442 171
Enzymes that do cut:
AarI AatII AccI AflII AflIII AloI AlwNI ApaI
ApoI AvaI AvrII BaeI BanI BanII BbeI BbsI
BbvCI BciVI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NarI NdeI NspI PasI
PciI PflMI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SapI SbfI SfcI SfoI SmlI SspI StyI
TaqII TatI TstI Tth111I XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AgeI AhdI AleI AlfI
ApaLI AscI AseI AsiSI BamHI BcgI BclI BfrBI
BglI BmtI BsaAI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BssHII BssSI BstAPI BstBI BstXI BstZ17I ClaI
CspCI DraIII EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KpnI MluI NaeI NcoI NgoMIV NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI XbaI XcmI XmaI XmnI