Restriction map for
pF1KB9559
(Linear) (Six-base) MAPSORT of: KIBB9559.seq Check: 2047 from: 1 to: 3258
KIBB9559.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AatII G_ACGT'C
Cuts at: 0 1821 3258
Size: 1821 1437
AccI GT'mk_AC
Cuts at: 0 1944 2214 3258
Size: 1944 270 1044
Fragments arranged by size:
1944 1044 270
Acc65I G'GTAC_C
Cuts at: 0 2257 3258
Size: 2257 1001
AclI AA'CG_TT
Cuts at: 0 972 3258
Size: 972 2286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 2623 3258
Size: 472 2151 635
Fragments arranged by size:
2151 635 472
AfeI AGC'GCT
Cuts at: 0 1384 3258
Size: 1384 1874
AflII C'TTAA_G
Cuts at: 0 45 3258
Size: 45 3213
AflIII A'CryG_T
Cuts at: 0 418 1736 2933 3258
Size: 418 1318 1197 325
Fragments arranged by size:
1318 1197 418 325
AgeI A'CCGG_T
Cuts at: 0 181 3258
Size: 181 3077
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1913 1945 2525 2557 2627 2659 3258
Size: 1913 32 580 32 70 32 599
Fragments arranged by size:
1913 599 580 70 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 859 2709 2823 3258
Size: 859 1850 114 435
Fragments arranged by size:
1850 859 435 114
ApaI G_GGCC'C
Cuts at: 0 1441 3258
Size: 1441 1817
ApoI r'AATT_y
Cuts at: 0 350 1069 2020 2156 2910 3028 3079 3258
Size: 350 719 951 136 754 118 51 179
Fragments arranged by size:
951 754 719 350 179 136 118 51
AseI AT'TA_AT
Cuts at: 0 83 725 3258
Size: 83 642 2533
Fragments arranged by size:
2533 642 83
AvaI C'yCGr_G
Cuts at: 0 1657 3019 3258
Size: 1657 1362 239
Fragments arranged by size:
1657 1362 239
BamHI G'GATC_C
Cuts at: 0 402 3258
Size: 402 2856
BanI G'GyrC_C
Cuts at: 0 2257 2416 3258
Size: 2257 159 842
Fragments arranged by size:
2257 842 159
BanII G_rGCy'C
Cuts at: 0 691 1441 3258
Size: 691 750 1817
Fragments arranged by size:
1817 750 691
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 415 1853 2936 3258
Size: 26 389 1438 1083 322
Fragments arranged by size:
1438 1083 389 322 26
BbvCI CC'TCA_GC
Cuts at: 0 1568 2870 3258
Size: 1568 1302 388
Fragments arranged by size:
1568 1302 388
BciVI GTATCCnnnnn_n'
Cuts at: 0 3006 3258
Size: 3006 252
BclI T'GATC_A
Cuts at: 0 2268 3258
Size: 2268 990
BfrBI ATG'CAT
Cuts at: 0 341 1812 3258
Size: 341 1471 1446
Fragments arranged by size:
1471 1446 341
BglI GCCn_nnn'nGGC
Cuts at: 0 1641 3258
Size: 1641 1617
BglII A'GATC_T
Cuts at: 0 3045 3258
Size: 3045 213
Bme1580I G_kGCm'C
Cuts at: 0 1441 3258
Size: 1441 1817
BmrI ACTGGGnnnn_n'
Cuts at: 0 2110 3258
Size: 2110 1148
BmtI G_CTAG'C
Cuts at: 0 2195 3258
Size: 2195 1063
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 370 3258
Size: 370 2888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 532 1142 1982 2845 3258
Size: 340 192 610 840 863 413
Fragments arranged by size:
863 840 610 413 340 192
Bpu10I CC'TnA_GC
Cuts at: 0 1124 1568 2870 3258
Size: 1124 444 1302 388
Fragments arranged by size:
1302 1124 444 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 3258
Size: 999 2259
BsaI GGTCTCn'nnnn_
Cuts at: 0 190 579 3258
Size: 190 389 2679
Fragments arranged by size:
2679 389 190
BsaHI Gr'CG_yC
Cuts at: 0 1818 2760 3258
Size: 1818 942 498
Fragments arranged by size:
1818 942 498
BsaWI w'CCGG_w
Cuts at: 0 181 3258
Size: 181 3077
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 497 527 1518 1548 1913 1943 1955 1985
Size: 497 30 991 30 365 30 12 30
Cuts at: 1985 3258
Size: 1273
Fragments arranged by size:
1273 991 497 365 30 30 30 30
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1526 1934 3140 3258
Size: 1526 408 1206 118
Fragments arranged by size:
1526 1206 408 118
BseYI C'CCAG_C
Cuts at: 0 253 1599 2208 2854 3258
Size: 253 1346 609 646 404
Fragments arranged by size:
1346 646 609 404 253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2351 2413 3258
Size: 2351 62 845
Fragments arranged by size:
2351 845 62
BsiHKAI G_wGCw'C
Cuts at: 0 326 691 820 2094 3258
Size: 326 365 129 1274 1164
Fragments arranged by size:
1274 1164 365 326 129
BsmI GAATG_Cn'
Cuts at: 0 1679 1782 3258
Size: 1679 103 1476
Fragments arranged by size:
1679 1476 103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 30 3258
Size: 30 3228
Bsp1286I G_dGCh'C
Cuts at: 0 326 691 820 1441 2094 3258
Size: 326 365 129 621 653 1164
Fragments arranged by size:
1164 653 621 365 326 129
BsrDI GCAATG_nn'
Cuts at: 0 154 3258
Size: 154 3104
BsrFI r'CCGG_y
Cuts at: 0 181 1865 3258
Size: 181 1684 1393
Fragments arranged by size:
1684 1393 181
BstEII G'GTnAC_C
Cuts at: 0 211 3258
Size: 211 3047
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2208 3006 3258
Size: 2208 798 252
Fragments arranged by size:
2208 798 252
BstYI r'GATC_y
Cuts at: 0 227 402 780 1008 1146 2642 3045 3258
Size: 227 175 378 228 138 1496 403 213
Fragments arranged by size:
1496 403 378 228 227 213 175 138
BstZ17I GTA'TAC
Cuts at: 0 2215 3258
Size: 2215 1043
Bsu36I CC'TnA_GG
Cuts at: 0 540 3258
Size: 540 2718
BtgI C'CryG_G
Cuts at: 0 2624 2646 3258
Size: 2624 22 612
Fragments arranged by size:
2624 612 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1311 3258
Size: 1311 1947
BtsI GCAGTG_nn'
Cuts at: 0 506 3258
Size: 506 2752
ClaI AT'CG_AT
Cuts at: 0 2768 3258
Size: 2768 490
DraI TTT'AAA
Cuts at: 0 969 3258
Size: 969 2289
DraIII CAC_nnn'GTG
Cuts at: 0 2692 3258
Size: 2692 566
DrdI GACnn_nn'nnGTC
Cuts at: 0 634 3058 3258
Size: 634 2424 200
Fragments arranged by size:
2424 634 200
EaeI y'GGCC_r
Cuts at: 0 1281 3258
Size: 1281 1977
EarI CTCTTCn'nnn_
Cuts at: 0 2003 2286 3258
Size: 2003 283 972
Fragments arranged by size:
2003 972 283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2666 3258
Size: 2666 592
EcoICRI GAG'CTC
Cuts at: 0 689 3258
Size: 689 2569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 472 532 1142 1982 2623 2845 3258
Size: 340 132 60 610 840 641 222 413
Fragments arranged by size:
840 641 610 413 340 222 132 60
EcoO109I rG'GnC_Cy
Cuts at: 0 257 308 1437 2233 3258
Size: 257 51 1129 796 1025
Fragments arranged by size:
1129 1025 796 257 51
EcoRI G'AATT_C
Cuts at: 0 350 3258
Size: 350 2908
EcoRV GAT'ATC
Cuts at: 0 1186 3258
Size: 1186 2072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2682 3258
Size: 2682 576
HaeII r_GCGC'y
Cuts at: 0 1386 3258
Size: 1386 1872
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 1551 1583 3258
Size: 559 32 960 32 1675
Fragments arranged by size:
1675 960 559 32 32
HincII GTy'rAC
Cuts at: 0 152 2121 3258
Size: 152 1969 1137
Fragments arranged by size:
1969 1137 152
HindIII A'AGCT_T
Cuts at: 0 1586 3258
Size: 1586 1672
KpnI G_GTAC'C
Cuts at: 0 2261 3258
Size: 2261 997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 199 3258
Size: 104 95 3059
Fragments arranged by size:
3059 104 95
MspA1I CmG'CkG
Cuts at: 0 1036 1219 1599 2682 3258
Size: 1036 183 380 1083 576
Fragments arranged by size:
1083 1036 576 380 183
NcoI C'CATG_G
Cuts at: 0 2624 3258
Size: 2624 634
NdeI CA'TA_TG
Cuts at: 0 1515 3258
Size: 1515 1743
NheI G'CTAG_C
Cuts at: 0 2191 3258
Size: 2191 1067
NsiI A_TGCA'T
Cuts at: 0 343 1814 3258
Size: 343 1471 1444
Fragments arranged by size:
1471 1444 343
NspI r_CATG'y
Cuts at: 0 206 422 1740 2592 3176 3258
Size: 206 216 1318 852 584 82
Fragments arranged by size:
1318 852 584 216 206 82
PasI CC'CwG_GG
Cuts at: 0 3156 3258
Size: 3156 102
PciI A'CATG_T
Cuts at: 0 418 1736 3258
Size: 418 1318 1522
Fragments arranged by size:
1522 1318 418
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 2625 3258
Size: 318 2307 633
Fragments arranged by size:
2307 633 318
PfoI T'CCnGG_A
Cuts at: 0 230 1841 3258
Size: 230 1611 1417
Fragments arranged by size:
1611 1417 230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1913 1945 3258
Size: 1913 32 1313
Fragments arranged by size:
1913 1313 32
PpuMI rG'GwC_Cy
Cuts at: 0 308 3258
Size: 308 2950
PshAI GACnn'nnGTC
Cuts at: 0 3245 3258
Size: 3245 13
PsiI TTA'TAA
Cuts at: 0 2489 3258
Size: 2489 769
PspOMI G'GGCC_C
Cuts at: 0 1437 3258
Size: 1437 1821
PstI C_TGCA'G
Cuts at: 0 1787 2726 3258
Size: 1787 939 532
Fragments arranged by size:
1787 939 532
PvuII CAG'CTG
Cuts at: 0 1036 1219 1599 2682 3258
Size: 1036 183 380 1083 576
Fragments arranged by size:
1083 1036 576 380 183
SacI G_AGCT'C
Cuts at: 0 691 3258
Size: 691 2567
SapI GCTCTTCn'nnn_
Cuts at: 0 2003 2286 3258
Size: 2003 283 972
Fragments arranged by size:
2003 972 283
ScaI AGT'ACT
Cuts at: 0 1163 1204 3258
Size: 1163 41 2054
Fragments arranged by size:
2054 1163 41
SfcI C'TryA_G
Cuts at: 0 1783 1945 2286 2722 3258
Size: 1783 162 341 436 536
Fragments arranged by size:
1783 536 436 341 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1641 3258
Size: 1641 1617
SmlI C'TyrA_G
Cuts at: 0 45 978 3258
Size: 45 933 2280
Fragments arranged by size:
2280 933 45
SphI G_CATG'C
Cuts at: 0 2592 3258
Size: 2592 666
SspI AAT'ATT
Cuts at: 0 1801 2946 3258
Size: 1801 1145 312
Fragments arranged by size:
1801 1145 312
StyI C'CwwG_G
Cuts at: 0 2624 2666 3258
Size: 2624 42 592
Fragments arranged by size:
2624 592 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2164 2404 2515 2822 3258
Size: 2164 240 111 307 436
Fragments arranged by size:
2164 436 307 240 111
TatI w'GTAC_w
Cuts at: 0 1131 1161 1202 2960 3003 3258
Size: 1131 30 41 1758 43 255
Fragments arranged by size:
1758 1131 255 43 41 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 497 529 1679 1711 3258
Size: 497 32 1150 32 1547
Fragments arranged by size:
1547 1150 497 32 32
XbaI T'CTAG_A
Cuts at: 0 714 3048 3258
Size: 714 2334 210
Fragments arranged by size:
2334 714 210
XmnI GAAnn'nnTTC
Cuts at: 0 1553 2012 2159 3258
Size: 1553 459 147 1099
Fragments arranged by size:
1553 1099 459 147
ZraI GAC'GTC
Cuts at: 0 1819 3258
Size: 1819 1439
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AloI AlwNI ApaI ApoI AseI AvaI BamHI
BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI
BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BsrDI BsrFI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII KpnI MmeI
MspA1I NcoI NdeI NheI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuII SacI SapI ScaI SfcI SfiI SmlI SphI
SspI StyI TaqII TatI TstI XbaI XmnI ZraI
Enzymes that do not cut:
AarI AhdI AleI AlfI ApaLI AscI AsiSI AvrII
BaeI BbeI BcgI BlpI BmgBI BsaAI BsaBI BsiEI
BsiWI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI CspCI EagI EcoNI FseI FspI FspAI
HpaI KasI MfeI MluI MscI MslI NaeI NarI
NgoMIV NotI NruI PacI PmeI PmlI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
Tth111I XcmI XhoI XmaI