Restriction map for
pF1KB9563
(Linear) (Six-base) MAPSORT of: KIBB9563.seq Check: 2768 from: 1 to: 6966
KIBB9563.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 785 2140 2280 3306 3819 4176 6142 6403
Size: 785 1355 140 1026 513 357 1966 261
Cuts at: 6403 6966
Size: 563
Fragments arranged by size:
1966 1355 1026 785 563 513 357 261
140
AatII G_ACGT'C
Cuts at: 0 6770 6966
Size: 6770 196
AccI GT'mk_AC
Cuts at: 0 2912 4529 4952 6966
Size: 2912 1617 423 2014
Fragments arranged by size:
2912 2014 1617 423
Acc65I G'GTAC_C
Cuts at: 0 805 2225 6966
Size: 805 1420 4741
Fragments arranged by size:
4741 1420 805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1981 3114 3736 4077 4771 5982 6054
Size: 599 1382 1133 622 341 694 1211 72
Cuts at: 6054 6966
Size: 912
Fragments arranged by size:
1382 1211 1133 912 694 622 599 341
72
AfeI AGC'GCT
Cuts at: 0 5224 6966
Size: 5224 1742
AflII C'TTAA_G
Cuts at: 0 6943 6966
Size: 6943 23
AflIII A'CryG_T
Cuts at: 0 6662 6966
Size: 6662 304
AhdI GACnn_n'nnGTC
Cuts at: 0 3529 4512 6966
Size: 3529 983 2454
Fragments arranged by size:
3529 2454 983
AleI CACnn'nnGTG
Cuts at: 0 334 824 1892 2678 2866 3442 4588 5366
Size: 334 490 1068 786 188 576 1146 778
Cuts at: 5366 5399 6862 6966
Size: 33 1463 104
Fragments arranged by size:
1463 1146 1068 786 778 576 490 334
188 104 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1152 2026 3683 4606 6966
Size: 1152 874 1657 923 2360
Fragments arranged by size:
2360 1657 1152 923 874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1900 1932 3362 3394 6966
Size: 1900 32 1430 32 3572
Fragments arranged by size:
3572 1900 1430 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1293 1404 3292 3573 4191 4767 4837 4851
Size: 1293 111 1888 281 618 576 70 14
Cuts at: 4851 5593 6777 6966
Size: 742 1184 189
Fragments arranged by size:
1888 1293 1184 742 618 576 281 189
111 70 14
ApaI G_GGCC'C
Cuts at: 0 626 2118 3703 4154 4876 5562 5669 6402
Size: 626 1492 1585 451 722 686 107 733
Cuts at: 6402 6881 6966
Size: 479 85
Fragments arranged by size:
1585 1492 733 722 686 626 479 451
107 85
ApaLI G'TGCA_C
Cuts at: 0 3280 3658 6966
Size: 3280 378 3308
Fragments arranged by size:
3308 3280 378
AvaI C'yCGr_G
Cuts at: 0 970 1418 1441 1455 1577 3089 3144 4030
Size: 970 448 23 14 122 1512 55 886
Cuts at: 4030 4990 5048 5146 5531 5555 6293 6310 6535
Size: 960 58 98 385 24 738 17 225
Cuts at: 6535 6966
Size: 431
Fragments arranged by size:
1512 970 960 886 738 448 431 385
225 122 98 58 55 24 23 17
14
BamHI G'GATC_C
Cuts at: 0 3305 6966
Size: 3305 3661
BanI G'GyrC_C
Cuts at: 0 117 708 805 1581 1786 2225 2724 3427
Size: 117 591 97 776 205 439 499 703
Cuts at: 3427 3877 4575 5374 5622 5841 5853 6172 6966
Size: 450 698 799 248 219 12 319 794
Fragments arranged by size:
799 794 776 703 698 591 499 450
439 319 248 219 205 117 97 12
BanII G_rGCy'C
Cuts at: 0 414 437 626 1442 1464 2118 3221 3703
Size: 414 23 189 816 22 654 1103 482
Cuts at: 3703 3841 4154 4215 4628 4876 4997 5027 5297
Size: 138 313 61 413 248 121 30 270
Cuts at: 5297 5562 5669 6135 6311 6402 6620 6881 6966
Size: 265 107 466 176 91 218 261 85
Fragments arranged by size:
1103 816 654 482 466 414 413 313
270 265 261 248 218 189 176 138
121 107 91 85 61 30 23 22
BbeI G_GCGC'C
Cuts at: 0 3881 4579 6966
Size: 3881 698 2387
Fragments arranged by size:
3881 2387 698
BbsI GAAGACnn'nnnn_
Cuts at: 0 1073 2894 3009 3738 6966
Size: 1073 1821 115 729 3228
Fragments arranged by size:
3228 1821 1073 729 115
BbvCI CC'TCA_GC
Cuts at: 0 280 396 937 3613 4177 4249 5498 5641
Size: 280 116 541 2676 564 72 1249 143
Cuts at: 5641 6966
Size: 1325
Fragments arranged by size:
2676 1325 1249 564 541 280 143 116
72
BfrBI ATG'CAT
Cuts at: 0 6103 6966
Size: 6103 863
BglI GCCn_nnn'nGGC
Cuts at: 0 724 1210 1719 2185 2599 3225 3605 3799
Size: 724 486 509 466 414 626 380 194
Cuts at: 3799 5434 5719 6966
Size: 1635 285 1247
Fragments arranged by size:
1635 1247 724 626 509 486 466 414
380 285 194
BglII A'GATC_T
Cuts at: 0 3737 6966
Size: 3737 3229
BlpI GC'TnA_GC
Cuts at: 0 1382 5755 6966
Size: 1382 4373 1211
Fragments arranged by size:
4373 1382 1211
Bme1580I G_kGCm'C
Cuts at: 0 451 626 658 1330 1428 1586 2118 2515
Size: 451 175 32 672 98 158 532 397
Cuts at: 2515 3284 3430 3662 3703 4154 4585 4876 5377
Size: 769 146 232 41 451 431 291 501
Cuts at: 5377 5406 5547 5562 5625 5669 5844 5858 6293
Size: 29 141 15 63 44 175 14 435
Cuts at: 6293 6402 6444 6881 6966
Size: 109 42 437 85
Fragments arranged by size:
769 672 532 501 451 451 437 435
431 397 291 232 175 175 158 146
141 109 98 85 63 44 42 41
32 29 15 14
BmgBI CAC'GTC
Cuts at: 0 170 1494 3123 3501 5055 6966
Size: 170 1324 1629 378 1554 1911
Fragments arranged by size:
1911 1629 1554 1324 378 170
BmrI ACTGGGnnnn_n'
Cuts at: 0 461 2120 2320 5949 6966
Size: 461 1659 200 3629 1017
Fragments arranged by size:
3629 1659 1017 461 200
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 952 1557 6966
Size: 952 605 5409
Fragments arranged by size:
5409 952 605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 278 967 988 1165 1579 2302 2386
Size: 133 145 689 21 177 414 723 84
Cuts at: 2386 2440 2452 3373 3381 3805 4002 4072 4213
Size: 54 12 921 8 424 197 70 141
Cuts at: 4213 4284 4809 5011 5395 5869 6277 6394 6451
Size: 71 525 202 384 474 408 117 57
Cuts at: 6451 6869 6966
Size: 418 97
Fragments arranged by size:
921 723 689 525 474 424 418 414
408 384 202 197 177 145 141 133
117 97 84 71 70 57 54 21
12 8
Bpu10I CC'TnA_GC
Cuts at: 0 280 396 937 1235 1250 2230 2308 3613
Size: 280 116 541 298 15 980 78 1305
Cuts at: 3613 4177 4249 5498 5641 6614 6643 6966
Size: 564 72 1249 143 973 29 323
Fragments arranged by size:
1305 1249 980 973 564 541 323 298
280 143 116 78 72 29 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1360 3398 4220 6373 6673 6966
Size: 1360 2038 822 2153 300 293
Fragments arranged by size:
2153 2038 1360 822 300 293
BsaI GGTCTCn'nnnn_
Cuts at: 0 1811 2059 2392 2926 5140 6811 6966
Size: 1811 248 333 534 2214 1671 155
Fragments arranged by size:
2214 1811 1671 534 333 248 155
BsaAI yAC'GTr
Cuts at: 0 2590 4296 6966
Size: 2590 1706 2670
Fragments arranged by size:
2670 2590 1706
BsaHI Gr'CG_yC
Cuts at: 0 243 3158 3878 4037 4576 6449 6767 6966
Size: 243 2915 720 159 539 1873 318 199
Fragments arranged by size:
2915 1873 720 539 318 243 199 159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1141 1171 3362 3392 3949 3979 5590 5620
Size: 1141 30 2191 30 557 30 1611 30
Cuts at: 5620 6037 6067 6686 6716 6966
Size: 417 30 619 30 250
Fragments arranged by size:
2191 1611 1141 619 557 417 250 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 384 529 944 1162 1165 1168 1782
Size: 289 95 145 415 218 3 3 614
Cuts at: 1782 2449 2707 3016 3178 4506 4732 5305 5865
Size: 667 258 309 162 1328 226 573 560
Cuts at: 5865 5932 6042 6045 6966
Size: 67 110 3 921
Fragments arranged by size:
1328 921 667 614 573 560 415 309
289 258 226 218 162 145 110 95
67 3 3 3
BseYI C'CCAG_C
Cuts at: 0 173 207 356 1053 1286 1716 2390 3161
Size: 173 34 149 697 233 430 674 771
Cuts at: 3161 3518 3563 3898 4202 4546 4610 4844 4877
Size: 357 45 335 304 344 64 234 33
Cuts at: 4877 4901 4957 5102 5110 5219 5263 5305 5330
Size: 24 56 145 8 109 44 42 25
Cuts at: 5330 5378 5561 6366 6966
Size: 48 183 805 600
Fragments arranged by size:
805 771 697 674 600 430 357 344
335 304 234 233 183 173 149 145
109 64 56 48 45 44 42 34
33 25 24 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1886 2308 2359 3079 3379 3934 3946
Size: 1375 511 422 51 720 300 555 12
Cuts at: 3946 5851 6966
Size: 1905 1115
Fragments arranged by size:
1905 1375 1115 720 555 511 422 300
51 12
BsiEI CG_ry'CG
Cuts at: 0 1841 1981 3443 6966
Size: 1841 140 1462 3523
Fragments arranged by size:
3523 1841 1462 140
BsiHKAI G_wGCw'C
Cuts at: 0 1442 2236 3284 3449 3662 4026 4498 4694
Size: 1442 794 1048 165 213 364 472 196
Cuts at: 4694 4742 5027 5243 6966
Size: 48 285 216 1723
Fragments arranged by size:
1723 1442 1048 794 472 364 285 216
213 196 165 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 295 1083 6966
Size: 295 788 5883
Fragments arranged by size:
5883 788 295
Bsp1286I G_dGCh'C
Cuts at: 0 414 437 451 626 658 1330 1428 1442
Size: 414 23 14 175 32 672 98 14
Cuts at: 1442 1464 1586 2118 2236 2515 3221 3284 3430
Size: 22 122 532 118 279 706 63 146
Cuts at: 3430 3449 3662 3703 3841 4026 4154 4215 4498
Size: 19 213 41 138 185 128 61 283
Cuts at: 4498 4585 4628 4694 4742 4876 4997 5027 5243
Size: 87 43 66 48 134 121 30 216
Cuts at: 5243 5297 5377 5406 5547 5562 5625 5669 5844
Size: 54 80 29 141 15 63 44 175
Cuts at: 5844 5858 6135 6293 6311 6402 6444 6620 6881
Size: 14 277 158 18 91 42 176 261
Cuts at: 6881 6966
Size: 85
Fragments arranged by size:
706 672 532 414 283 279 277 261
216 213 185 176 175 175 158 146
141 138 134 128 122 121 118 98
91 87 85 80 66 63 63 61
54 48 44 43 42 41 32 30
29 23 22 19 18 15 14 14
14
BspHI T'CATG_A
Cuts at: 0 6060 6966
Size: 6060 906
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 240 785 2140 2280 3306 3581 3819
Size: 158 82 545 1355 140 1026 275 238
Cuts at: 3819 3968 4176 6142 6403 6966
Size: 149 208 1966 261 563
Fragments arranged by size:
1966 1355 1026 563 545 275 261 238
208 158 149 140 82
BsrBI CCG'CTC
Cuts at: 0 2814 5490 6855 6966
Size: 2814 2676 1365 111
Fragments arranged by size:
2814 2676 1365 111
BsrDI GCAATG_nn'
Cuts at: 0 3686 6966
Size: 3686 3280
BsrFI r'CCGG_y
Cuts at: 0 701 734 881 1132 1833 1867 1980 2903
Size: 701 33 147 251 701 34 113 923
Cuts at: 2903 3850 4886 6966
Size: 947 1036 2080
Fragments arranged by size:
2080 1036 947 923 701 701 251 147
113 34 33
BssSI C'ACGA_G
Cuts at: 0 2719 4627 5540 6966
Size: 2719 1908 913 1426
Fragments arranged by size:
2719 1908 1426 913
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3573 4983 5238 6966
Size: 3573 1410 255 1728
Fragments arranged by size:
3573 1728 1410 255
BstBI TT'CG_AA
Cuts at: 0 922 6966
Size: 922 6044
BstEII G'GTnAC_C
Cuts at: 0 2568 2683 6966
Size: 2568 115 4283
Fragments arranged by size:
4283 2568 115
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1783 1894 2076 2680 3405 4170 5199 5368
Size: 1783 111 182 604 725 765 1029 169
Cuts at: 5368 5386 5796 6690 6966
Size: 18 410 894 276
Fragments arranged by size:
1783 1029 894 765 725 604 410 276
182 169 111 18
BstYI r'GATC_y
Cuts at: 0 1991 2303 3074 3305 3737 4521 6966
Size: 1991 312 771 231 432 784 2445
Fragments arranged by size:
2445 1991 784 771 432 312 231
Bsu36I CC'TnA_GG
Cuts at: 0 475 496 1208 1724 2729 3256 3974 4011
Size: 475 21 712 516 1005 527 718 37
Cuts at: 4011 4103 4813 6966
Size: 92 710 2153
Fragments arranged by size:
2153 1005 718 712 710 527 516 475
92 37 21
BtgI C'CryG_G
Cuts at: 0 13 83 128 785 896 952 1223 1431
Size: 13 70 45 657 111 56 271 208
Cuts at: 1431 1805 1890 2075 2264 2823 3297 3629 3641
Size: 374 85 185 189 559 474 332 12
Cuts at: 3641 5171 5361 5432 6160 6599 6966
Size: 1530 190 71 728 439 367
Fragments arranged by size:
1530 728 657 559 474 439 374 367
332 271 208 190 189 185 111 85
71 70 56 45 13 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1943 3149 4001 6966
Size: 1943 1206 852 2965
Fragments arranged by size:
2965 1943 1206 852
BtsI GCAGTG_nn'
Cuts at: 0 2371 2988 3341 3483 3809 3941 4132 4463
Size: 2371 617 353 142 326 132 191 331
Cuts at: 4463 6240 6966
Size: 1777 726
Fragments arranged by size:
2371 1777 726 617 353 331 326 191
142 132
DraIII CAC_nnn'GTG
Cuts at: 0 333 2351 3847 6966
Size: 333 2018 1496 3119
Fragments arranged by size:
3119 2018 1496 333
DrdI GACnn_nn'nnGTC
Cuts at: 0 1075 4330 6196 6772 6966
Size: 1075 3255 1866 576 194
Fragments arranged by size:
3255 1866 1075 576 194
EaeI y'GGCC_r
Cuts at: 0 68 125 528 836 1083 1216 1316 1952
Size: 68 57 403 308 247 133 100 636
Cuts at: 1952 1970 1978 1982 2529 2562 2901 3026 3206
Size: 18 8 4 547 33 339 125 180
Cuts at: 3206 3386 3440 5057 5201 5720 6218 6273 6626
Size: 180 54 1617 144 519 498 55 353
Cuts at: 6626 6870 6951 6966
Size: 244 81 15
Fragments arranged by size:
1617 636 547 519 498 403 353 339
308 247 244 180 180 144 133 125
100 81 68 57 55 54 33 18
15 8 4
EagI C'GGCC_G
Cuts at: 0 1978 3440 6966
Size: 1978 1462 3526
Fragments arranged by size:
3526 1978 1462
EarI CTCTTCn'nnn_
Cuts at: 0 575 3256 3829 4271 5248 6733 6966
Size: 575 2681 573 442 977 1485 233
Fragments arranged by size:
2681 1485 977 575 573 442 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4663 5808 6238 6966
Size: 4663 1145 430 728
Fragments arranged by size:
4663 1145 728 430
EcoICRI GAG'CTC
Cuts at: 0 1440 5025 6966
Size: 1440 3585 1941
Fragments arranged by size:
3585 1941 1440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 278 599 967 988 1165 1579 1981
Size: 133 145 321 368 21 177 414 402
Cuts at: 1981 2302 2386 2440 2452 3114 3373 3381 3736
Size: 321 84 54 12 662 259 8 355
Cuts at: 3736 3805 4002 4072 4077 4213 4284 4771 4809
Size: 69 197 70 5 136 71 487 38
Cuts at: 4809 5011 5395 5869 5982 6054 6277 6394 6451
Size: 202 384 474 113 72 223 117 57
Cuts at: 6451 6869 6966
Size: 418 97
Fragments arranged by size:
662 487 474 418 414 402 384 368
355 321 321 259 223 202 197 177
145 136 133 117 113 97 84 72
71 70 69 57 54 38 21 12
8 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 474 1253 1375 2434 2800 3416 4219 5944
Size: 474 779 122 1059 366 616 803 1725
Cuts at: 5944 6256 6887 6966
Size: 312 631 79
Fragments arranged by size:
1725 1059 803 779 631 616 474 366
312 122 79
EcoO109I rG'GnC_Cy
Cuts at: 0 423 455 623 1720 1764 1779 2114 2115
Size: 423 32 168 1097 44 15 335 1
Cuts at: 2115 3183 3252 3570 3699 3951 4151 4244 4872
Size: 1068 69 318 129 252 200 93 628
Cuts at: 4872 4873 4923 5142 5426 5665 5950 6262 6348
Size: 1 50 219 284 239 285 312 86
Cuts at: 6348 6398 6399 6595 6774 6877 6878 6966
Size: 50 1 196 179 103 1 88
Fragments arranged by size:
1097 1068 628 423 335 318 312 285
284 252 239 219 200 196 179 168
129 103 93 88 86 69 50 50
44 32 15 1 1 1 1
EcoRV GAT'ATC
Cuts at: 0 2949 6966
Size: 2949 4017
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 205 1530 2515 6654 6966
Size: 205 1325 985 4139 312
Fragments arranged by size:
4139 1325 985 312 205
FseI GG_CCGG'CC
Cuts at: 0 1984 6966
Size: 1984 4982
FspI TGC'GCA
Cuts at: 0 1390 4594 6966
Size: 1390 3204 2372
Fragments arranged by size:
3204 2372 1390
HaeII r_GCGC'y
Cuts at: 0 1815 3881 4256 4579 5109 5226 5874 6712
Size: 1815 2066 375 323 530 117 648 838
Cuts at: 6712 6966
Size: 254
Fragments arranged by size:
2066 1815 838 648 530 375 323 254
117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1735 1767 3014 3046 3127 3159 3515 3547
Size: 1735 32 1247 32 81 32 356 32
Cuts at: 3547 3979 3994 4011 4026 4492 4498 4524 4530
Size: 432 15 17 15 466 6 26 6
Cuts at: 4530 5569 5601 6966
Size: 1039 32 1365
Fragments arranged by size:
1735 1365 1247 1039 466 432 356 81
32 32 32 32 32 26 17 15
15 6 6
HincII GTy'rAC
Cuts at: 0 1638 6966
Size: 1638 5328
HindIII A'AGCT_T
Cuts at: 0 1106 1187 5861 6966
Size: 1106 81 4674 1105
Fragments arranged by size:
4674 1106 1105 81
KasI G'GCGC_C
Cuts at: 0 3877 4575 6966
Size: 3877 698 2391
Fragments arranged by size:
3877 2391 698
KpnI G_GTAC'C
Cuts at: 0 809 2229 6966
Size: 809 1420 4737
Fragments arranged by size:
4737 1420 809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1568 1841 3347 6966
Size: 1568 273 1506 3619
Fragments arranged by size:
3619 1568 1506 273
MscI TGG'CCA
Cuts at: 0 70 127 1085 1218 1972 2531 3028 3388
Size: 70 57 958 133 754 559 497 360
Cuts at: 3388 5203 5722 6220 6628 6872 6953 6966
Size: 1815 519 498 408 244 81 13
Fragments arranged by size:
1815 958 754 559 519 498 497 408
360 244 133 81 70 57 13
MslI CAynn'nnrTG
Cuts at: 0 334 754 824 872 1892 2678 2866 3442
Size: 334 420 70 48 1020 786 188 576
Cuts at: 3442 3665 4588 5366 5399 5470 5827 6682 6862
Size: 223 923 778 33 71 357 855 180
Cuts at: 6862 6966
Size: 104
Fragments arranged by size:
1020 923 855 786 778 576 420 357
334 223 188 180 104 71 70 48
33
MspA1I CmG'CkG
Cuts at: 0 15 85 156 211 1290 1551 1716 1892
Size: 15 70 71 55 1079 261 165 176
Cuts at: 1892 2199 3165 3312 3508 3763 3783 4066 4284
Size: 307 966 147 196 255 20 283 218
Cuts at: 4284 4614 5031 5157 5277 5334 5565 5619 5754
Size: 330 417 126 120 57 231 54 135
Cuts at: 5754 5835 6324 6622 6862 6966
Size: 81 489 298 240 104
Fragments arranged by size:
1079 966 489 417 330 307 298 283
261 255 240 231 218 196 176 165
147 135 126 120 104 81 71 70
57 55 54 20 15
NaeI GCC'GGC
Cuts at: 0 703 736 1134 1835 1869 1982 2905 3852
Size: 703 33 398 701 34 113 923 947
Cuts at: 3852 4888 6966
Size: 1036 2078
Fragments arranged by size:
2078 1036 947 923 703 701 398 113
34 33
NarI GG'CG_CC
Cuts at: 0 3878 4576 6966
Size: 3878 698 2390
Fragments arranged by size:
3878 2390 698
NcoI C'CATG_G
Cuts at: 0 1223 1431 2823 3629 6160 6599 6966
Size: 1223 208 1392 806 2531 439 367
Fragments arranged by size:
2531 1392 1223 806 439 367 208
NgoMIV G'CCGG_C
Cuts at: 0 701 734 1132 1833 1867 1980 2903 3850
Size: 701 33 398 701 34 113 923 947
Cuts at: 3850 4886 6966
Size: 1036 2080
Fragments arranged by size:
2080 1036 947 923 701 701 398 113
34 33
NsiI A_TGCA'T
Cuts at: 0 6105 6966
Size: 6105 861
NspI r_CATG'y
Cuts at: 0 1354 5763 6145 6666 6966
Size: 1354 4409 382 521 300
Fragments arranged by size:
4409 1354 521 382 300
PasI CC'CwG_GG
Cuts at: 0 609 4780 6168 6966
Size: 609 4171 1388 798
Fragments arranged by size:
4171 1388 798 609
PciI A'CATG_T
Cuts at: 0 6662 6966
Size: 6662 304
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 633 2351 4377 5280 5865 6327 6966
Size: 333 300 1718 2026 903 585 462 639
Fragments arranged by size:
2026 1718 903 639 585 462 333 300
PfoI T'CCnGG_A
Cuts at: 0 1004 1487 1694 4261 6254 6885 6966
Size: 1004 483 207 2567 1993 631 81
Fragments arranged by size:
2567 1993 1004 631 483 207 81
PmlI CAC'GTG
Cuts at: 0 2590 6966
Size: 2590 4376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 3362 3394 6966
Size: 379 32 2951 32 3572
Fragments arranged by size:
3572 2951 379 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 423 455 1779 3183 3252 3570 3951 4244
Size: 423 32 1324 1404 69 318 381 293
Cuts at: 4244 5142 5950 6262 6348 6774 6966
Size: 898 808 312 86 426 192
Fragments arranged by size:
1404 1324 898 808 426 423 381 318
312 293 192 86 69 32
PspOMI G'GGCC_C
Cuts at: 0 622 2114 3699 4150 4872 5558 5665 6398
Size: 622 1492 1585 451 722 686 107 733
Cuts at: 6398 6877 6966
Size: 479 89
Fragments arranged by size:
1585 1492 733 722 686 622 479 451
107 89
PspXI vC'TCGA_Gb
Cuts at: 0 1441 4030 6966
Size: 1441 2589 2936
Fragments arranged by size:
2936 2589 1441
PstI C_TGCA'G
Cuts at: 0 155 161 230 251 290 1141 1940 2123
Size: 155 6 69 21 39 851 799 183
Cuts at: 2123 2138 3317 3816 4187 4220 4448 5327 5343
Size: 15 1179 499 371 33 228 879 16
Cuts at: 5343 6917 6966
Size: 1574 49
Fragments arranged by size:
1574 1179 879 851 799 499 371 228
183 155 69 49 39 33 21 16
15 6
PvuII CAG'CTG
Cuts at: 0 156 211 1290 1551 3165 3312 3508 3763
Size: 156 55 1079 261 1614 147 196 255
Cuts at: 3763 3783 4284 4614 5031 5277 5334 5565 5754
Size: 20 501 330 417 246 57 231 189
Cuts at: 5754 5835 6324 6862 6966
Size: 81 489 538 104
Fragments arranged by size:
1614 1079 538 501 489 417 330 261
255 246 231 196 189 156 147 104
81 57 55 20
SacI G_AGCT'C
Cuts at: 0 1442 5027 6966
Size: 1442 3585 1939
Fragments arranged by size:
3585 1939 1442
SacII CC_GC'GG
Cuts at: 0 16 86 1893 6966
Size: 16 70 1807 5073
Fragments arranged by size:
5073 1807 70 16
SanDI GG'GwC_CC
Cuts at: 0 455 5142 5950 6262 6966
Size: 455 4687 808 312 704
Fragments arranged by size:
4687 808 704 455 312
SapI GCTCTTCn'nnn_
Cuts at: 0 3829 4271 5248 6733 6966
Size: 3829 442 977 1485 233
Fragments arranged by size:
3829 1485 977 442 233
SbfI CC_TGCA'GG
Cuts at: 0 251 4187 6966
Size: 251 3936 2779
Fragments arranged by size:
3936 2779 251
ScaI AGT'ACT
Cuts at: 0 3272 6958 6966
Size: 3272 3686 8
Fragments arranged by size:
3686 3272 8
SexAI A'CCwGG_T
Cuts at: 0 3857 6966
Size: 3857 3109
SfcI C'TryA_G
Cuts at: 0 151 157 226 247 286 1137 1936 2119
Size: 151 6 69 21 39 851 799 183
Cuts at: 2119 2134 2506 3113 3313 3594 3812 4183 4216
Size: 15 372 607 200 281 218 371 33
Cuts at: 4216 4444 5323 5339 6510 6913 6966
Size: 228 879 16 1171 403 53
Fragments arranged by size:
1171 879 851 799 607 403 372 371
281 228 218 200 183 151 69 53
39 33 21 16 15 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5719 6966
Size: 5719 1247
SfoI GGC'GCC
Cuts at: 0 3879 4577 6966
Size: 3879 698 2389
Fragments arranged by size:
3879 2389 698
SmaI CCC'GGG
Cuts at: 0 1457 3091 5050 5557 6312 6966
Size: 1457 1634 1959 507 755 654
Fragments arranged by size:
1959 1634 1457 755 654 507
SmlI C'TyrA_G
Cuts at: 0 1339 1441 3413 4030 4235 6352 6652 6943
Size: 1339 102 1972 617 205 2117 300 291
Cuts at: 6943 6966
Size: 23
Fragments arranged by size:
2117 1972 1339 617 300 291 205 102
23
SspI AAT'ATT
Cuts at: 0 4965 6966
Size: 4965 2001
StuI AGG'CCT
Cuts at: 0 991 1738 3364 3979 4016 4442 5458 5509
Size: 991 747 1626 615 37 426 1016 51
Cuts at: 5509 6966
Size: 1457
Fragments arranged by size:
1626 1457 1016 991 747 615 426 51
37
StyI C'CwwG_G
Cuts at: 0 44 459 713 1223 1431 1782 2022 2823
Size: 44 415 254 510 208 351 240 801
Cuts at: 2823 3629 3881 6160 6599 6629 6966
Size: 806 252 2279 439 30 337
Fragments arranged by size:
2279 806 801 510 439 415 351 337
254 252 240 208 44 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 1300 1855 2404 2557 3259 3912 4009
Size: 154 1146 555 549 153 702 653 97
Cuts at: 4009 5392 5641 6945 6951 6966
Size: 1383 249 1304 6 15
Fragments arranged by size:
1383 1304 1146 702 653 555 549 249
154 153 97 15 6
TatI w'GTAC_w
Cuts at: 0 3270 6956 6966
Size: 3270 3686 10
Fragments arranged by size:
3686 3270 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2174 2206 6434 6466 6966
Size: 2174 32 4228 32 500
Fragments arranged by size:
4228 2174 500 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 1962 2058 2415 3348 3505 5070 5169
Size: 600 1362 96 357 933 157 1565 99
Cuts at: 5169 6183 6966
Size: 1014 783
Fragments arranged by size:
1565 1362 1014 933 783 600 357 157
99 96
XhoI C'TCGA_G
Cuts at: 0 1441 4030 6966
Size: 1441 2589 2936
Fragments arranged by size:
2936 2589 1441
XmaI C'CCGG_G
Cuts at: 0 1455 3089 5048 5555 6310 6966
Size: 1455 1634 1959 507 755 656
Fragments arranged by size:
1959 1634 1455 755 656 507
ZraI GAC'GTC
Cuts at: 0 6768 6966
Size: 6768 198
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SspI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AclI AgeI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BmtI BsaBI BsaWI BsiWI BsmI
BspEI BsrGI BssHII BstZ17I ClaI CspCI DraI EcoRI
FspAI HpaI MfeI MluI NdeI NheI NotI NruI
PacI PmeI PshAI PsiI PsrI PvuI RsrII SalI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XmnI