Restriction map for
pF1KB9564
(Linear) (Six-base) MAPSORT of: KIBB9564.seq Check: 6434 from: 1 to: 729
KIBB9564.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AccI GT'mk_AC
Cuts at: 0 422 729
Size: 422 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 508 729
Size: 382 126 221
Fragments arranged by size:
382 221 126
AlwNI CAG_nnn'CTG
Cuts at: 0 318 360 729
Size: 318 42 369
Fragments arranged by size:
369 318 42
ApaI G_GGCC'C
Cuts at: 0 414 558 630 711 729
Size: 414 144 72 81 18
Fragments arranged by size:
414 144 81 72 18
AvaI C'yCGr_G
Cuts at: 0 551 614 634 729
Size: 551 63 20 95
Fragments arranged by size:
551 95 63 20
BamHI G'GATC_C
Cuts at: 0 473 729
Size: 473 256
BanI G'GyrC_C
Cuts at: 0 534 683 729
Size: 534 149 46
Fragments arranged by size:
534 149 46
BanII G_rGCy'C
Cuts at: 0 414 558 630 711 729
Size: 414 144 72 81 18
Fragments arranged by size:
414 144 81 72 18
BbeI G_GCGC'C
Cuts at: 0 687 729
Size: 687 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 230 729
Size: 230 499
BglI GCCn_nnn'nGGC
Cuts at: 0 625 706 729
Size: 625 81 23
Fragments arranged by size:
625 81 23
BlpI GC'TnA_GC
Cuts at: 0 367 595 729
Size: 367 228 134
Fragments arranged by size:
367 228 134
Bme1580I G_kGCm'C
Cuts at: 0 414 558 630 711 729
Size: 414 144 72 81 18
Fragments arranged by size:
414 144 81 72 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 142 729
Size: 93 49 587
Fragments arranged by size:
587 93 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 208 729
Size: 208 521
BsaHI Gr'CG_yC
Cuts at: 0 684 729
Size: 684 45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 433 685 729
Size: 70 363 252 44
Fragments arranged by size:
363 252 70 44
BseYI C'CCAG_C
Cuts at: 0 465 729
Size: 465 264
BsmI GAATG_Cn'
Cuts at: 0 327 729
Size: 327 402
Bsp1286I G_dGCh'C
Cuts at: 0 414 558 630 711 729
Size: 414 144 72 81 18
Fragments arranged by size:
414 144 81 72 18
BsrBI CCG'CTC
Cuts at: 0 697 729
Size: 697 32
BsrFI r'CCGG_y
Cuts at: 0 508 599 729
Size: 508 91 130
Fragments arranged by size:
508 130 91
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 225 279 729
Size: 57 168 54 450
Fragments arranged by size:
450 168 57 54
BstYI r'GATC_y
Cuts at: 0 473 729
Size: 473 256
BtgI C'CryG_G
Cuts at: 0 25 729
Size: 25 704
BtsI GCAGTG_nn'
Cuts at: 0 720 729
Size: 720 9
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 235 270 729
Size: 235 35 459
Fragments arranged by size:
459 235 35
DraIII CAC_nnn'GTG
Cuts at: 0 228 729
Size: 228 501
EaeI y'GGCC_r
Cuts at: 0 375 506 698 729
Size: 375 131 192 31
Fragments arranged by size:
375 192 131 31
EarI CTCTTCn'nnn_
Cuts at: 0 130 729
Size: 130 599
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 486 729
Size: 486 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 508 729
Size: 382 126 221
Fragments arranged by size:
382 221 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 729
Size: 352 377
EcoO109I rG'GnC_Cy
Cuts at: 0 357 410 411 483 555 626 707 729
Size: 357 53 1 72 72 71 81 22
Fragments arranged by size:
357 81 72 72 71 53 22 1
HaeII r_GCGC'y
Cuts at: 0 687 729
Size: 687 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 729
Size: 226 32 471
Fragments arranged by size:
471 226 32
HindIII A'AGCT_T
Cuts at: 0 490 729
Size: 490 239
KasI G'GCGC_C
Cuts at: 0 683 729
Size: 683 46
MscI TGG'CCA
Cuts at: 0 377 729
Size: 377 352
MslI CAynn'nnrTG
Cuts at: 0 55 277 729
Size: 55 222 452
Fragments arranged by size:
452 222 55
MspA1I CmG'CkG
Cuts at: 0 315 729
Size: 315 414
NaeI GCC'GGC
Cuts at: 0 510 601 729
Size: 510 91 128
Fragments arranged by size:
510 128 91
NarI GG'CG_CC
Cuts at: 0 684 729
Size: 684 45
NcoI C'CATG_G
Cuts at: 0 25 729
Size: 25 704
NgoMIV G'CCGG_C
Cuts at: 0 508 599 729
Size: 508 91 130
Fragments arranged by size:
508 130 91
NspI r_CATG'y
Cuts at: 0 40 729
Size: 40 689
PasI CC'CwG_GG
Cuts at: 0 354 729
Size: 354 375
PspOMI G'GGCC_C
Cuts at: 0 410 554 626 707 729
Size: 410 144 72 81 22
Fragments arranged by size:
410 144 81 72 22
PspXI vC'TCGA_Gb
Cuts at: 0 634 729
Size: 634 95
PvuII CAG'CTG
Cuts at: 0 315 729
Size: 315 414
SexAI A'CCwGG_T
Cuts at: 0 224 729
Size: 224 505
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 706 729
Size: 706 23
SfoI GGC'GCC
Cuts at: 0 685 729
Size: 685 44
SmaI CCC'GGG
Cuts at: 0 553 729
Size: 553 176
SmlI C'TyrA_G
Cuts at: 0 634 729
Size: 634 95
StyI C'CwwG_G
Cuts at: 0 25 326 641 701 729
Size: 25 301 315 60 28
Fragments arranged by size:
315 301 60 28 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 23 55 729
Size: 23 32 674
Fragments arranged by size:
674 32 23
XhoI C'TCGA_G
Cuts at: 0 634 729
Size: 634 95
XmaI C'CCGG_G
Cuts at: 0 551 729
Size: 551 178
Enzymes that do cut:
AccI AcuI AlwNI ApaI AvaI BamHI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BmrI BsaI BsaHI
BseRI BseYI BsmI Bsp1286I BsrBI BsrFI BstXI BstYI
BtgI BtsI CspCI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PspOMI
PspXI PvuII SexAI SfiI SfoI SmaI SmlI StyI
TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DrdI
EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XcmI XmnI
ZraI