(Linear) (Six-base) MAPSORT of: KIBB9566.seq Check: 2136 from: 1 to: 2415 KIBB9566.orf With 182 enzymes: SgfI * March 3, 2008 11:10 .. AccI GT'mk_AC Cuts at: 0 806 2076 2415 Size: 806 1270 339 Fragments arranged by size: 1270 806 339 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 329 353 581 2006 2348 2415 Size: 329 24 228 1425 342 67 Fragments arranged by size: 1425 342 329 228 67 24 AflIII A'CryG_T Cuts at: 0 393 417 435 444 453 462 1994 2037 Size: 393 24 18 9 9 9 1532 43 Cuts at: 2037 2415 Size: 378 Fragments arranged by size: 1532 393 378 43 24 18 9 9 9 AgeI A'CCGG_T Cuts at: 0 2327 2415 Size: 2327 88 AhdI GACnn_n'nnGTC Cuts at: 0 702 2415 Size: 702 1713 AleI CACnn'nnGTG Cuts at: 0 1899 2330 2415 Size: 1899 431 85 Fragments arranged by size: 1899 431 85 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1883 2080 2415 Size: 1883 197 335 Fragments arranged by size: 1883 335 197 AlwNI CAG_nnn'CTG Cuts at: 0 61 2415 Size: 61 2354 ApaLI G'TGCA_C Cuts at: 0 1024 2415 Size: 1024 1391 ApoI r'AATT_y Cuts at: 0 1564 2415 Size: 1564 851 BamHI G'GATC_C Cuts at: 0 584 2415 Size: 584 1831 BanII G_rGCy'C Cuts at: 0 1688 2415 Size: 1688 727 BbsI GAAGACnn'nnnn_ Cuts at: 0 1212 1822 2221 2364 2415 Size: 1212 610 399 143 51 Fragments arranged by size: 1212 610 399 143 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1333 1367 2415 Size: 1333 34 1048 Fragments arranged by size: 1333 1048 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1839 2415 Size: 1839 576 BclI T'GATC_A Cuts at: 0 84 834 855 2415 Size: 84 750 21 1560 Fragments arranged by size: 1560 750 84 21 Bme1580I G_kGCm'C Cuts at: 0 235 1028 1140 2415 Size: 235 793 112 1275 Fragments arranged by size: 1275 793 235 112 BmgBI CAC'GTC Cuts at: 0 396 2415 Size: 396 2019 BmrI ACTGGGnnnn_n' Cuts at: 0 1721 2415 Size: 1721 694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 54 2415 Size: 54 2361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 800 1444 2415 Size: 800 644 971 Fragments arranged by size: 971 800 644 Bpu10I CC'TnA_GC Cuts at: 0 281 2415 Size: 281 2134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1917 2014 2415 Size: 1917 97 401 Fragments arranged by size: 1917 401 97 BsaAI yAC'GTr Cuts at: 0 2040 2415 Size: 2040 375 BsaBI GATnn'nnATC Cuts at: 0 83 1256 2415 Size: 83 1173 1159 Fragments arranged by size: 1173 1159 83 BsaWI w'CCGG_w Cuts at: 0 1118 2327 2415 Size: 1118 1209 88 Fragments arranged by size: 1209 1118 88 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1708 1738 2415 Size: 1708 30 677 Fragments arranged by size: 1708 677 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1003 2415 Size: 1003 1412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1879 2415 Size: 1879 536 BsiHKAI G_wGCw'C Cuts at: 0 1028 1688 2415 Size: 1028 660 727 Fragments arranged by size: 1028 727 660 BsmI GAATG_Cn' Cuts at: 0 1471 2415 Size: 1471 944 Bsp1286I G_dGCh'C Cuts at: 0 235 1028 1140 1688 2415 Size: 235 793 112 548 727 Fragments arranged by size: 793 727 548 235 112 BspHI T'CATG_A Cuts at: 0 471 2415 Size: 471 1944 BsrDI GCAATG_nn' Cuts at: 0 327 1908 2415 Size: 327 1581 507 Fragments arranged by size: 1581 507 327 BsrFI r'CCGG_y Cuts at: 0 2327 2415 Size: 2327 88 BsrGI T'GTAC_A Cuts at: 0 758 2415 Size: 758 1657 BssSI C'ACGA_G Cuts at: 0 1027 2415 Size: 1027 1388 BstAPI GCAn_nnn'nTGC Cuts at: 0 553 2385 2415 Size: 553 1832 30 Fragments arranged by size: 1832 553 30 BstBI TT'CG_AA Cuts at: 0 1927 2415 Size: 1927 488 BstYI r'GATC_y Cuts at: 0 584 2100 2145 2415 Size: 584 1516 45 270 Fragments arranged by size: 1516 584 270 45 Bsu36I CC'TnA_GG Cuts at: 0 743 2277 2415 Size: 743 1534 138 Fragments arranged by size: 1534 743 138 BtgI C'CryG_G Cuts at: 0 692 2415 Size: 692 1723 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1484 2415 Size: 1484 931 BtsI GCAGTG_nn' Cuts at: 0 1127 2415 Size: 1127 1288 DraI TTT'AAA Cuts at: 0 768 938 2286 2415 Size: 768 170 1348 129 Fragments arranged by size: 1348 768 170 129 DraIII CAC_nnn'GTG Cuts at: 0 2224 2415 Size: 2224 191 EarI CTCTTCn'nnn_ Cuts at: 0 1767 2415 Size: 1767 648 EcoICRI GAG'CTC Cuts at: 0 1686 2415 Size: 1686 729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 329 353 581 800 1444 2006 2348 2415 Size: 329 24 228 219 644 562 342 67 Fragments arranged by size: 644 562 342 329 228 219 67 24 EcoRV GAT'ATC Cuts at: 0 240 2415 Size: 240 2175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 31 2367 2415 Size: 31 2336 48 Fragments arranged by size: 2336 48 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 541 573 688 720 1257 1289 2085 2117 Size: 541 32 115 32 537 32 796 32 Cuts at: 2117 2415 Size: 298 Fragments arranged by size: 796 541 537 298 115 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 411 420 429 686 2415 Size: 411 9 9 257 1729 Fragments arranged by size: 1729 411 257 9 9 MslI CAynn'nnrTG Cuts at: 0 77 148 476 754 1151 1646 1815 1899 Size: 77 71 328 278 397 495 169 84 Cuts at: 1899 1988 2157 2330 2415 Size: 89 169 173 85 Fragments arranged by size: 495 397 328 278 173 169 169 89 85 84 77 71 MspA1I CmG'CkG Cuts at: 0 1009 1370 1585 2415 Size: 1009 361 215 830 Fragments arranged by size: 1009 830 361 215 NcoI C'CATG_G Cuts at: 0 692 2415 Size: 692 1723 NdeI CA'TA_TG Cuts at: 0 2210 2415 Size: 2210 205 NspI r_CATG'y Cuts at: 0 421 439 448 457 466 1783 1870 1998 Size: 421 18 9 9 9 1317 87 128 Cuts at: 1998 2415 Size: 417 Fragments arranged by size: 1317 421 417 128 87 18 9 9 9 PasI CC'CwG_GG Cuts at: 0 881 2415 Size: 881 1534 PciI A'CATG_T Cuts at: 0 417 435 444 453 462 1994 2415 Size: 417 18 9 9 9 1532 421 Fragments arranged by size: 1532 421 417 18 9 9 9 PflMI CCAn_nnn'nTGG Cuts at: 0 1614 2415 Size: 1614 801 PmlI CAC'GTG Cuts at: 0 2040 2415 Size: 2040 375 PshAI GACnn'nnGTC Cuts at: 0 1826 2415 Size: 1826 589 PstI C_TGCA'G Cuts at: 0 1002 1758 2415 Size: 1002 756 657 Fragments arranged by size: 1002 756 657 PvuII CAG'CTG Cuts at: 0 1585 2415 Size: 1585 830 SacI G_AGCT'C Cuts at: 0 1688 2415 Size: 1688 727 ScaI AGT'ACT Cuts at: 0 1738 2251 2338 2415 Size: 1738 513 87 77 Fragments arranged by size: 1738 513 87 77 SfcI C'TryA_G Cuts at: 0 803 998 1754 2267 2415 Size: 803 195 756 513 148 Fragments arranged by size: 803 756 513 195 148 SgrAI Cr'CCGG_yG Cuts at: 0 2327 2415 Size: 2327 88 SmlI C'TyrA_G Cuts at: 0 1932 2029 2415 Size: 1932 97 386 Fragments arranged by size: 1932 386 97 StuI AGG'CCT Cuts at: 0 2019 2415 Size: 2019 396 StyI C'CwwG_G Cuts at: 0 644 692 2415 Size: 644 48 1723 Fragments arranged by size: 1723 644 48 SwaI ATTT'AAAT Cuts at: 0 938 2415 Size: 938 1477 TatI w'GTAC_w Cuts at: 0 102 758 1372 1736 2249 2336 2415 Size: 102 656 614 364 513 87 79 Fragments arranged by size: 656 614 513 364 102 87 79 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2284 2316 2415 Size: 2284 32 99 Fragments arranged by size: 2284 99 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 888 2415 Size: 888 1527 XmnI GAAnn'nnTTC Cuts at: 0 224 1486 2350 2361 2415 Size: 224 1262 864 11 54 Fragments arranged by size: 1262 864 224 54 11 Enzymes that do cut: AccI AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI BamHI BanII BbsI BcgI BciVI BclI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EarI EcoICRI Eco57MI EcoRV FalI Hin4I MmeI MslI MspA1I NcoI NdeI NspI PasI PciI PflMI PmlI PshAI PstI PvuII SacI ScaI SfcI SgrAI SmlI StuI StyI SwaI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BfrBI BglI BglII BlpI BmtI BsaI BsaHI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BssHII BstEII BstXI BstZ17I ClaI CspCI DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI TaqII Tth111I XbaI XhoI XmaI ZraI