Restriction map for
pF1KB9568
(Linear) (Six-base) MAPSORT of: KIBB9568.seq Check: 3813 from: 1 to: 4335
KIBB9568.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1540 4335
Size: 1540 2795
AatII G_ACGT'C
Cuts at: 0 767 2444 4335
Size: 767 1677 1891
Fragments arranged by size:
1891 1677 767
Acc65I G'GTAC_C
Cuts at: 0 2929 4335
Size: 2929 1406
AclI AA'CG_TT
Cuts at: 0 650 1583 4335
Size: 650 933 2752
Fragments arranged by size:
2752 933 650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 727 1429 3416 3635 4335
Size: 622 105 702 1987 219 700
Fragments arranged by size:
1987 702 700 622 219 105
AfeI AGC'GCT
Cuts at: 0 2576 2916 4335
Size: 2576 340 1419
Fragments arranged by size:
2576 1419 340
AleI CACnn'nnGTG
Cuts at: 0 4042 4335
Size: 4042 293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2251 2786 4241 4335
Size: 2251 535 1455 94
Fragments arranged by size:
2251 1455 535 94
AlwNI CAG_nnn'CTG
Cuts at: 0 677 705 1195 1695 2301 3178 3904 4335
Size: 677 28 490 500 606 877 726 431
Fragments arranged by size:
877 726 677 606 500 490 431 28
ApaI G_GGCC'C
Cuts at: 0 1042 2257 4335
Size: 1042 1215 2078
Fragments arranged by size:
2078 1215 1042
ApaLI G'TGCA_C
Cuts at: 0 4045 4335
Size: 4045 290
ApoI r'AATT_y
Cuts at: 0 2498 3487 4335
Size: 2498 989 848
Fragments arranged by size:
2498 989 848
AvaI C'yCGr_G
Cuts at: 0 1901 3097 3277 4335
Size: 1901 1196 180 1058
Fragments arranged by size:
1901 1196 1058 180
BamHI G'GATC_C
Cuts at: 0 1089 2127 4335
Size: 1089 1038 2208
Fragments arranged by size:
2208 1089 1038
BanI G'GyrC_C
Cuts at: 0 559 1371 1603 2929 3033 3067 3219 4335
Size: 559 812 232 1326 104 34 152 1116
Fragments arranged by size:
1326 1116 812 559 232 152 104 34
BanII G_rGCy'C
Cuts at: 0 227 497 1042 1679 2144 2219 2257 2609
Size: 227 270 545 637 465 75 38 352
Cuts at: 2609 2704 2824 2978 3337 3577 4335
Size: 95 120 154 359 240 758
Fragments arranged by size:
758 637 545 465 359 352 270 240
227 154 120 95 75 38
BbeI G_GCGC'C
Cuts at: 0 3037 3223 4335
Size: 3037 186 1112
Fragments arranged by size:
3037 1112 186
BbsI GAAGACnn'nnnn_
Cuts at: 0 882 4335
Size: 882 3453
BbvCI CC'TCA_GC
Cuts at: 0 2911 4335
Size: 2911 1424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 669 703 2329 2363 3717 3751 4335
Size: 669 34 1626 34 1354 34 584
Fragments arranged by size:
1626 1354 669 584 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3403 4335
Size: 3403 932
BfrBI ATG'CAT
Cuts at: 0 366 4335
Size: 366 3969
BglI GCCn_nnn'nGGC
Cuts at: 0 397 3121 3257 4335
Size: 397 2724 136 1078
Fragments arranged by size:
2724 1078 397 136
BlpI GC'TnA_GC
Cuts at: 0 2044 2323 2614 3905 4335
Size: 2044 279 291 1291 430
Fragments arranged by size:
2044 1291 430 291 279
Bme1580I G_kGCm'C
Cuts at: 0 1042 1257 1606 2257 4049 4335
Size: 1042 215 349 651 1792 286
Fragments arranged by size:
1792 1042 651 349 286 215
BmgBI CAC'GTC
Cuts at: 0 2647 4335
Size: 2647 1688
BmrI ACTGGGnnnn_n'
Cuts at: 0 800 2941 3070 4335
Size: 800 2141 129 1265
Fragments arranged by size:
2141 1265 800 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1906 2233 2841 3591 4335
Size: 1906 327 608 750 744
Fragments arranged by size:
1906 750 744 608 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 2140 2180 2516 2534 2962 3336 3348
Size: 280 1860 40 336 18 428 374 12
Cuts at: 3348 3698 4335
Size: 350 637
Fragments arranged by size:
1860 637 428 374 350 336 280 40
18 12
Bpu10I CC'TnA_GC
Cuts at: 0 2603 2911 4000 4268 4335
Size: 2603 308 1089 268 67
Fragments arranged by size:
2603 1089 308 268 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 1306 4335
Size: 106 1200 3029
Fragments arranged by size:
3029 1200 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 2006 4335
Size: 2006 2329
BsaAI yAC'GTr
Cuts at: 0 1368 1389 4335
Size: 1368 21 2946
Fragments arranged by size:
2946 1368 21
BsaBI GATnn'nnATC
Cuts at: 0 679 4335
Size: 679 3656
BsaHI Gr'CG_yC
Cuts at: 0 764 2441 2749 3034 3220 4335
Size: 764 1677 308 285 186 1115
Fragments arranged by size:
1677 1115 764 308 285 186
BsaWI w'CCGG_w
Cuts at: 0 502 1614 2899 2926 3021 4106 4335
Size: 502 1112 1285 27 95 1085 229
Fragments arranged by size:
1285 1112 1085 502 229 95 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2003 2033 2506 2536 3949 3979 4335
Size: 2003 30 473 30 1413 30 356
Fragments arranged by size:
2003 1413 473 356 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 94 1074 1482 2274 2814 2826 3115
Size: 19 75 980 408 792 540 12 289
Cuts at: 3115 3339 4335
Size: 224 996
Fragments arranged by size:
996 980 792 540 408 289 224 75
19 12
BseYI C'CCAG_C
Cuts at: 0 667 698 986 1688 1874 1981 2257 2350
Size: 667 31 288 702 186 107 276 93
Cuts at: 2350 2622 2880 3040 3151 4335
Size: 272 258 160 111 1184
Fragments arranged by size:
1184 702 667 288 276 272 258 186
160 111 107 93 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 820 1031 1444 2766 4335
Size: 76 744 211 413 1322 1569
Fragments arranged by size:
1569 1322 744 413 211 76
BsiEI CG_ry'CG
Cuts at: 0 2892 4335
Size: 2892 1443
BsiHKAI G_wGCw'C
Cuts at: 0 227 2050 2824 2978 3385 3754 4049 4335
Size: 227 1823 774 154 407 369 295 286
Fragments arranged by size:
1823 774 407 369 295 286 227 154
BsiWI C'GTAC_G
Cuts at: 0 995 4335
Size: 995 3340
BsmI GAATG_Cn'
Cuts at: 0 525 3798 4335
Size: 525 3273 537
Fragments arranged by size:
3273 537 525
BsmBI CGTCTCn'nnnn_
Cuts at: 0 384 1391 2443 2662 4335
Size: 384 1007 1052 219 1673
Fragments arranged by size:
1673 1052 1007 384 219
Bsp1286I G_dGCh'C
Cuts at: 0 227 497 1042 1257 1606 1679 2050 2144
Size: 227 270 545 215 349 73 371 94
Cuts at: 2144 2219 2257 2609 2704 2824 2978 3337 3385
Size: 75 38 352 95 120 154 359 48
Cuts at: 3385 3577 3754 4049 4335
Size: 192 177 295 286
Fragments arranged by size:
545 371 359 352 349 295 286 270
227 215 192 177 154 120 95 94
75 73 48 38
BspEI T'CCGG_A
Cuts at: 0 502 1614 4106 4335
Size: 502 1112 2492 229
Fragments arranged by size:
2492 1112 502 229
BspHI T'CATG_A
Cuts at: 0 4310 4335
Size: 4310 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 1466 1540 2423 4335
Size: 98 1368 74 883 1912
Fragments arranged by size:
1912 1368 883 98 74
BsrBI CCG'CTC
Cuts at: 0 749 1650 1656 2888 4335
Size: 749 901 6 1232 1447
Fragments arranged by size:
1447 1232 901 749 6
BsrDI GCAATG_nn'
Cuts at: 0 422 3939 4335
Size: 422 3517 396
Fragments arranged by size:
3517 422 396
BsrFI r'CCGG_y
Cuts at: 0 67 590 693 2032 2891 3973 4335
Size: 67 523 103 1339 859 1082 362
Fragments arranged by size:
1339 1082 859 523 362 103 67
BsrGI T'GTAC_A
Cuts at: 0 629 932 4335
Size: 629 303 3403
Fragments arranged by size:
3403 629 303
BstAPI GCAn_nnn'nTGC
Cuts at: 0 677 4335
Size: 677 3658
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2182 2868 3633 4335
Size: 2182 686 765 702
Fragments arranged by size:
2182 765 702 686
BstYI r'GATC_y
Cuts at: 0 641 674 831 1089 1305 1593 2127 3849
Size: 641 33 157 258 216 288 534 1722
Cuts at: 3849 4335
Size: 486
Fragments arranged by size:
1722 641 534 486 288 258 216 157
33
BtgI C'CryG_G
Cuts at: 0 12 555 942 2867 4229 4335
Size: 12 543 387 1925 1362 106
Fragments arranged by size:
1925 1362 543 387 106 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 33 3531 4335
Size: 33 3498 804
Fragments arranged by size:
3498 804 33
BtsI GCAGTG_nn'
Cuts at: 0 315 1176 1950 4230 4335
Size: 315 861 774 2280 105
Fragments arranged by size:
2280 861 774 315 105
ClaI AT'CG_AT
Cuts at: 0 2489 4335
Size: 2489 1846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1335 1370 4335
Size: 1335 35 2965
Fragments arranged by size:
2965 1335 35
DraIII CAC_nnn'GTG
Cuts at: 0 948 1371 1467 2685 4335
Size: 948 423 96 1218 1650
Fragments arranged by size:
1650 1218 948 423 96
EaeI y'GGCC_r
Cuts at: 0 217 752 1124 1179 2889 3122 3623 3635
Size: 217 535 372 55 1710 233 501 12
Cuts at: 3635 3686 3975 4335
Size: 51 289 360
Fragments arranged by size:
1710 535 501 372 360 289 233 217
55 51 12
EagI C'GGCC_G
Cuts at: 0 2889 4335
Size: 2889 1446
EarI CTCTTCn'nnn_
Cuts at: 0 1211 2832 4105 4335
Size: 1211 1621 1273 230
Fragments arranged by size:
1621 1273 1211 230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 763 1707 3083 3538 4335
Size: 763 944 1376 455 797
Fragments arranged by size:
1376 944 797 763 455
EcoICRI GAG'CTC
Cuts at: 0 225 2822 2976 4335
Size: 225 2597 154 1359
Fragments arranged by size:
2597 1359 225 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 622 727 1429 2140 2180 2516 2534
Size: 280 342 105 702 711 40 336 18
Cuts at: 2534 2962 3336 3348 3416 3635 3698 4335
Size: 428 374 12 68 219 63 637
Fragments arranged by size:
711 702 637 428 374 342 336 280
219 105 68 63 40 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 1197 1867 2243 4015 4335
Size: 103 1094 670 376 1772 320
Fragments arranged by size:
1772 1094 670 376 320 103
EcoO109I rG'GnC_Cy
Cuts at: 0 399 1038 1039 1245 1358 1481 1501 1522
Size: 399 639 1 206 113 123 20 21
Cuts at: 1522 2053 2253 2254 2475 3105 3113 3611 3996
Size: 531 200 1 221 630 8 498 385
Cuts at: 3996 4335
Size: 339
Fragments arranged by size:
639 630 531 498 399 385 339 221
206 200 123 113 21 20 8 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4119 4335
Size: 4119 216
FseI GG_CCGG'CC
Cuts at: 0 2895 4335
Size: 2895 1440
HaeII r_GCGC'y
Cuts at: 0 392 1224 2578 2918 3037 3223 4335
Size: 392 832 1354 340 119 186 1112
Fragments arranged by size:
1354 1112 832 392 340 186 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1323 1355 2661 2693 2728 2760 3949 3981
Size: 1323 32 1306 32 35 32 1189 32
Cuts at: 3981 4071 4103 4335
Size: 90 32 232
Fragments arranged by size:
1323 1306 1189 232 90 35 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 4293 4335
Size: 4293 42
HindIII A'AGCT_T
Cuts at: 0 187 4335
Size: 187 4148
KasI G'GCGC_C
Cuts at: 0 3033 3219 4335
Size: 3033 186 1116
Fragments arranged by size:
3033 1116 186
KpnI G_GTAC'C
Cuts at: 0 2933 4335
Size: 2933 1402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 4335
Size: 1253 3082
MscI TGG'CCA
Cuts at: 0 219 3124 3625 3637 3688 4335
Size: 219 2905 501 12 51 647
Fragments arranged by size:
2905 647 501 219 51 12
MslI CAynn'nnrTG
Cuts at: 0 52 115 208 343 1391 2996 3550 3631
Size: 52 63 93 135 1048 1605 554 81
Cuts at: 3631 4042 4335
Size: 411 293
Fragments arranged by size:
1605 1048 554 411 293 135 93 81
63 52
MspA1I CmG'CkG
Cuts at: 0 1281 1841 2361 2437 2613 3162 3377 3663
Size: 1281 560 520 76 176 549 215 286
Cuts at: 3663 3904 4335
Size: 241 431
Fragments arranged by size:
1281 560 549 520 431 286 241 215
176 76
NaeI GCC'GGC
Cuts at: 0 69 592 695 2034 2893 4335
Size: 69 523 103 1339 859 1442
Fragments arranged by size:
1442 1339 859 523 103 69
NarI GG'CG_CC
Cuts at: 0 3034 3220 4335
Size: 3034 186 1115
Fragments arranged by size:
3034 1115 186
NcoI C'CATG_G
Cuts at: 0 12 2867 4335
Size: 12 2855 1468
Fragments arranged by size:
2855 1468 12
NdeI CA'TA_TG
Cuts at: 0 259 4335
Size: 259 4076
NgoMIV G'CCGG_C
Cuts at: 0 67 590 693 2032 2891 4335
Size: 67 523 103 1339 859 1444
Fragments arranged by size:
1444 1339 859 523 103 67
NsiI A_TGCA'T
Cuts at: 0 368 4335
Size: 368 3967
NspI r_CATG'y
Cuts at: 0 120 304 366 4335
Size: 120 184 62 3969
Fragments arranged by size:
3969 184 120 62
PasI CC'CwG_GG
Cuts at: 0 2301 2803 4065 4335
Size: 2301 502 1262 270
Fragments arranged by size:
2301 1262 502 270
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 303 2847 3512 4335
Size: 18 285 2544 665 823
Fragments arranged by size:
2544 823 665 285 18
PfoI T'CCnGG_A
Cuts at: 0 637 1308 2007 2117 4016 4335
Size: 637 671 699 110 1899 319
Fragments arranged by size:
1899 699 671 637 319 110
PmlI CAC'GTG
Cuts at: 0 1368 1389 4335
Size: 1368 21 2946
Fragments arranged by size:
2946 1368 21
PpuMI rG'GwC_Cy
Cuts at: 0 1358 1481 1522 2475 3105 4335
Size: 1358 123 41 953 630 1230
Fragments arranged by size:
1358 1230 953 630 123 41
PspOMI G'GGCC_C
Cuts at: 0 1038 2253 4335
Size: 1038 1215 2082
Fragments arranged by size:
2082 1215 1038
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 466 498 4335
Size: 466 32 3837
Fragments arranged by size:
3837 466 32
PstI C_TGCA'G
Cuts at: 0 322 782 1851 2835 3257 3662 4335
Size: 322 460 1069 984 422 405 673
Fragments arranged by size:
1069 984 673 460 422 405 322
PvuII CAG'CTG
Cuts at: 0 1281 2361 3663 3904 4335
Size: 1281 1080 1302 241 431
Fragments arranged by size:
1302 1281 1080 431 241
SacI G_AGCT'C
Cuts at: 0 227 2824 2978 4335
Size: 227 2597 154 1357
Fragments arranged by size:
2597 1357 227 154
SanDI GG'GwC_CC
Cuts at: 0 1358 1481 1522 4335
Size: 1358 123 41 2813
Fragments arranged by size:
2813 1358 123 41
SapI GCTCTTCn'nnn_
Cuts at: 0 1211 4105 4335
Size: 1211 2894 230
Fragments arranged by size:
2894 1211 230
SbfI CC_TGCA'GG
Cuts at: 0 3257 4335
Size: 3257 1078
SexAI A'CCwGG_T
Cuts at: 0 146 2015 4335
Size: 146 1869 2320
Fragments arranged by size:
2320 1869 146
SfcI C'TryA_G
Cuts at: 0 100 318 778 1187 1847 2831 3253 3658
Size: 100 218 460 409 660 984 422 405
Cuts at: 3658 4335
Size: 677
Fragments arranged by size:
984 677 660 460 422 409 405 218
100
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3121 3257 4335
Size: 3121 136 1078
Fragments arranged by size:
3121 1078 136
SfoI GGC'GCC
Cuts at: 0 3035 3221 4335
Size: 3035 186 1114
Fragments arranged by size:
3035 1114 186
SmaI CCC'GGG
Cuts at: 0 3279 4335
Size: 3279 1056
SmlI C'TyrA_G
Cuts at: 0 121 1321 4335
Size: 121 1200 3014
Fragments arranged by size:
3014 1200 121
SphI G_CATG'C
Cuts at: 0 120 4335
Size: 120 4215
SrfI GCCC'GGGC
Cuts at: 0 3279 4335
Size: 3279 1056
StuI AGG'CCT
Cuts at: 0 1732 3052 3251 4335
Size: 1732 1320 199 1084
Fragments arranged by size:
1732 1320 1084 199
StyI C'CwwG_G
Cuts at: 0 12 1476 1626 2867 3425 3785 3812 4145
Size: 12 1464 150 1241 558 360 27 333
Cuts at: 4145 4335
Size: 190
Fragments arranged by size:
1464 1241 558 360 333 190 150 27
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1755 2835 4335
Size: 1755 1080 1500
Fragments arranged by size:
1755 1500 1080
TatI w'GTAC_w
Cuts at: 0 629 932 1349 4335
Size: 629 303 417 2986
Fragments arranged by size:
2986 629 417 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 235 267 1596 1628 2506 2538 2889 2921
Size: 235 32 1329 32 878 32 351 32
Cuts at: 2921 3638 3670 4225 4257 4335
Size: 717 32 555 32 78
Fragments arranged by size:
1329 878 717 555 351 235 78 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1485 4335
Size: 1485 2850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 983 1285 1917 2023 3810 4335
Size: 983 302 632 106 1787 525
Fragments arranged by size:
1787 983 632 525 302 106
XmaI C'CCGG_G
Cuts at: 0 3277 4335
Size: 3277 1058
XmnI GAAnn'nnTTC
Cuts at: 0 4099 4335
Size: 4099 236
ZraI GAC'GTC
Cuts at: 0 765 2442 4335
Size: 765 1677 1893
Fragments arranged by size:
1893 1677 765
Enzymes that do cut:
AarI AatII Acc65I AclI AcuI AfeI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI
HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PsrI
PstI PvuII SacI SanDI SapI SbfI SexAI SfcI
SfiI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AflII AflIII AgeI AhdI AloI AscI AseI
AsiSI AvrII BaeI BclI BglII BmtI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I DraI DrdI EcoRI EcoRV
FspI FspAI HpaI MfeI MluI NheI NotI NruI
PacI PciI PmeI PpiI PshAI PsiI PspXI PvuI
RsrII SacII SalI ScaI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI