Restriction map for
pF1KB9571
(Linear) (Six-base) MAPSORT of: KIBB9571.seq Check: 3862 from: 1 to: 954
KIBB9571.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 264 954
Size: 264 690
AccI GT'mk_AC
Cuts at: 0 10 954
Size: 10 944
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 954
Size: 422 532
AflII C'TTAA_G
Cuts at: 0 739 954
Size: 739 215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 383 845 954
Size: 383 462 109
Fragments arranged by size:
462 383 109
AlwNI CAG_nnn'CTG
Cuts at: 0 469 954
Size: 469 485
ApoI r'AATT_y
Cuts at: 0 782 954
Size: 782 172
AseI AT'TA_AT
Cuts at: 0 91 954
Size: 91 863
AvaI C'yCGr_G
Cuts at: 0 755 954
Size: 755 199
BamHI G'GATC_C
Cuts at: 0 903 954
Size: 903 51
BanI G'GyrC_C
Cuts at: 0 442 821 954
Size: 442 379 133
Fragments arranged by size:
442 379 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 799 833 954
Size: 799 34 121
Fragments arranged by size:
799 121 34
BclI T'GATC_A
Cuts at: 0 482 954
Size: 482 472
BglI GCCn_nnn'nGGC
Cuts at: 0 290 505 954
Size: 290 215 449
Fragments arranged by size:
449 290 215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 698 954
Size: 436 262 256
Fragments arranged by size:
436 262 256
BsaAI yAC'GTr
Cuts at: 0 711 954
Size: 711 243
BsaBI GATnn'nnATC
Cuts at: 0 250 310 487 954
Size: 250 60 177 467
Fragments arranged by size:
467 250 177 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 605 635 954
Size: 605 30 319
Fragments arranged by size:
605 319 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 759 954
Size: 759 195
BseYI C'CCAG_C
Cuts at: 0 299 492 954
Size: 299 193 462
Fragments arranged by size:
462 299 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 292 298 954
Size: 118 174 6 656
Fragments arranged by size:
656 174 118 6
BsiHKAI G_wGCw'C
Cuts at: 0 516 954
Size: 516 438
BsmI GAATG_Cn'
Cuts at: 0 720 954
Size: 720 234
Bsp1286I G_dGCh'C
Cuts at: 0 516 954
Size: 516 438
BspHI T'CATG_A
Cuts at: 0 623 954
Size: 623 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 63 90 264 815 954
Size: 63 27 174 551 139
Fragments arranged by size:
551 174 139 63 27
BsrDI GCAATG_nn'
Cuts at: 0 603 935 954
Size: 603 332 19
Fragments arranged by size:
603 332 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 397 954
Size: 397 557
BstYI r'GATC_y
Cuts at: 0 245 251 903 954
Size: 245 6 652 51
Fragments arranged by size:
652 245 51 6
BstZ17I GTA'TAC
Cuts at: 0 11 954
Size: 11 943
BtgI C'CryG_G
Cuts at: 0 833 954
Size: 833 121
BtsI GCAGTG_nn'
Cuts at: 0 335 561 954
Size: 335 226 393
Fragments arranged by size:
393 335 226
EaeI y'GGCC_r
Cuts at: 0 132 342 954
Size: 132 210 612
Fragments arranged by size:
612 210 132
EarI CTCTTCn'nnn_
Cuts at: 0 692 954
Size: 692 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 436 698 954
Size: 422 14 262 256
Fragments arranged by size:
422 262 256 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 473 954
Size: 473 481
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 605 637 954
Size: 605 32 317
Fragments arranged by size:
605 317 32
MscI TGG'CCA
Cuts at: 0 134 344 954
Size: 134 210 610
Fragments arranged by size:
610 210 134
MslI CAynn'nnrTG
Cuts at: 0 827 946 954
Size: 827 119 8
Fragments arranged by size:
827 119 8
NcoI C'CATG_G
Cuts at: 0 833 954
Size: 833 121
NdeI CA'TA_TG
Cuts at: 0 896 954
Size: 896 58
PshAI GACnn'nnGTC
Cuts at: 0 331 415 954
Size: 331 84 539
Fragments arranged by size:
539 331 84
SapI GCTCTTCn'nnn_
Cuts at: 0 692 954
Size: 692 262
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 290 954
Size: 290 664
SmlI C'TyrA_G
Cuts at: 0 739 954
Size: 739 215
StyI C'CwwG_G
Cuts at: 0 78 833 954
Size: 78 755 121
Fragments arranged by size:
755 121 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 254 286 371 403 834 866 926 954
Size: 254 32 85 32 431 32 60 28
Fragments arranged by size:
431 254 85 60 32 32 32 28
Tth111I GACn'n_nGTC
Cuts at: 0 170 954
Size: 170 784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 157 256 954
Size: 157 99 698
Fragments arranged by size:
698 157 99
Enzymes that do cut:
AarI AccI AcuI AflII AlfI AlwNI ApoI AseI
AvaI BamHI BanI BcgI BclI BglI BpmI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BstAPI BstYI BstZ17I BtgI BtsI
EaeI EarI Eco57MI EcoNI Hin4I MscI MslI NcoI
NdeI PshAI SapI SfiI SmlI StyI TstI Tth111I
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BanII
BbeI BbsI BbvCI BciVI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaHI
BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI XbaI XhoI
XmaI XmnI ZraI