(Linear) (Six-base) MAPSORT of: KIBB9573.seq  Check: 987  from: 1  to: 1125



KIBB9573.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     713    1125 
   Size:        713     412
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     592    1125 
   Size:        592     533
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     648     680    1125 
   Size:        648      32     445

  Fragments arranged by size: 

                 648     445      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     355    1125 
   Size:        355     770
 
ApoI r'AATT_y

Cuts at:      0     451     540     671    1125 
   Size:        451      89     131     454

  Fragments arranged by size: 

                 454     451     131      89
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     719    1125 
   Size:        719     406
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     272    1125 
   Size:        272     853
 
BfrBI ATG'CAT

Cuts at:      0     552    1125 
   Size:        552     573
 
BlpI GC'TnA_GC

Cuts at:      0     650    1125 
   Size:        650     475
 
Bme1580I G_kGCm'C

Cuts at:      0     420    1125 
   Size:        420     705
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     112     136     139     142     145     148     772 
   Size:        103       9      24       3       3       3       3     624

Cuts at:    772    1125 
   Size:        353

  Fragments arranged by size: 

                 624     353     103      24       9       3       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0      44    1125 
   Size:         44    1081
 
Bsp1286I G_dGCh'C

Cuts at:      0     420    1125 
   Size:        420     705
 
BsrDI GCAATG_nn'

Cuts at:      0     574    1125 
   Size:        574     551
 
BstYI r'GATC_y

Cuts at:      0     533    1125 
   Size:        533     592
 
EaeI y'GGCC_r

Cuts at:      0     998    1125 
   Size:        998     127
 
EarI CTCTTCn'nnn_

Cuts at:      0      98     216     523     716     749     962    1125 
   Size:         98     118     307     193      33     213     163

  Fragments arranged by size: 

                 307     213     193     163     118      98      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     713    1125 
   Size:        713     412
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     552    1125 
   Size:        552     573
 
HincII GTy'rAC

Cuts at:      0     197    1125 
   Size:        197     928
 
MfeI C'AATT_G

Cuts at:      0     236    1125 
   Size:        236     889
 
MscI TGG'CCA

Cuts at:      0    1000    1125 
   Size:       1000     125
 
NsiI A_TGCA'T

Cuts at:      0     554    1125 
   Size:        554     571
 
PacI TTA_AT'TAA

Cuts at:      0     938    1125 
   Size:        938     187
 
SfcI C'TryA_G

Cuts at:      0     959    1125 
   Size:        959     166
 
SpeI A'CTAG_T

Cuts at:      0     522    1125 
   Size:        522     603
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      58    1125 
   Size:         58    1067
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1008    1125 
   Size:       1008     117
 

 Enzymes that do cut:

     AcuI     AlfI     AloI    AlwNI     ApoI     BbsI    BciVI    BfrBI
     BlpI Bme1580I    BseRI    BseYI Bsp1286I    BsrDI    BstYI     EaeI
     EarI  Eco57MI     FalI   HincII     MfeI     MscI     NsiI     PacI
     SfcI     SpeI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI     BglI    BglII    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI