Restriction map for
pF1KB9574
(Linear) (Six-base) MAPSORT of: KIBB9574.seq Check: 2764 from: 1 to: 1140
KIBB9574.orf
With 182 enzymes: SgfI *
February 20, 2009 16:43 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 811 1140
Size: 580 231 329
Fragments arranged by size:
580 329 231
AflIII A'CryG_T
Cuts at: 0 1010 1140
Size: 1010 130
AleI CACnn'nnGTG
Cuts at: 0 976 1140
Size: 976 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 474 1140
Size: 474 666
AlwNI CAG_nnn'CTG
Cuts at: 0 225 1140
Size: 225 915
ApaI G_GGCC'C
Cuts at: 0 944 1140
Size: 944 196
ApaLI G'TGCA_C
Cuts at: 0 969 1140
Size: 969 171
AvaI C'yCGr_G
Cuts at: 0 9 1036 1140
Size: 9 1027 104
Fragments arranged by size:
1027 104 9
BanII G_rGCy'C
Cuts at: 0 124 276 746 803 944 965 1140
Size: 124 152 470 57 141 21 175
Fragments arranged by size:
470 175 152 141 124 57 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 343 799 1007 1017 1140
Size: 343 456 208 10 123
Fragments arranged by size:
456 343 208 123 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 590 624 1140
Size: 590 34 516
Fragments arranged by size:
590 516 34
BlpI GC'TnA_GC
Cuts at: 0 1114 1140
Size: 1114 26
Bme1580I G_kGCm'C
Cuts at: 0 944 973 1140
Size: 944 29 167
Fragments arranged by size:
944 167 29
BmgBI CAC'GTC
Cuts at: 0 1013 1140
Size: 1013 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 680 833 915 1140
Size: 680 153 82 225
Fragments arranged by size:
680 225 153 82
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1062 1119 1140
Size: 1062 57 21
Fragments arranged by size:
1062 57 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 150 303 383 979 1140
Size: 96 54 153 80 596 161
Fragments arranged by size:
596 161 153 96 80 54
Bpu10I CC'TnA_GC
Cuts at: 0 740 1140
Size: 740 400
BsaHI Gr'CG_yC
Cuts at: 0 34 1140
Size: 34 1106
BsaWI w'CCGG_w
Cuts at: 0 210 467 975 1140
Size: 210 257 508 165
Fragments arranged by size:
508 257 210 165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 910 940 952 975 982 1005
Size: 94 30 786 30 12 23 7 23
Cuts at: 1005 1140
Size: 135
Fragments arranged by size:
786 135 94 30 30 23 23 12
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 237 486 718 721 1140
Size: 237 249 232 3 419
Fragments arranged by size:
419 249 237 232 3
BseYI C'CCAG_C
Cuts at: 0 307 822 994 1054 1140
Size: 307 515 172 60 86
Fragments arranged by size:
515 307 172 86 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 250 901 984 1082 1140
Size: 132 118 651 83 98 58
Fragments arranged by size:
651 132 118 98 83 58
BsiEI CG_ry'CG
Cuts at: 0 68 1140
Size: 68 1072
BsiHKAI G_wGCw'C
Cuts at: 0 973 1140
Size: 973 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 168 249 1140
Size: 168 81 891
Fragments arranged by size:
891 168 81
Bsp1286I G_dGCh'C
Cuts at: 0 124 276 746 803 944 965 973 1140
Size: 124 152 470 57 141 21 8 167
Fragments arranged by size:
470 167 152 141 124 57 21 8
BspEI T'CCGG_A
Cuts at: 0 210 1140
Size: 210 930
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 563 612 1140
Size: 224 339 49 528
Fragments arranged by size:
528 339 224 49
BsrBI CCG'CTC
Cuts at: 0 860 1140
Size: 860 280
BsrDI GCAATG_nn'
Cuts at: 0 1098 1140
Size: 1098 42
BstEII G'GTnAC_C
Cuts at: 0 981 1140
Size: 981 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 957 1131 1140
Size: 957 174 9
Fragments arranged by size:
957 174 9
BstYI r'GATC_y
Cuts at: 0 614 1140
Size: 614 526
BtgI C'CryG_G
Cuts at: 0 185 944 1140
Size: 185 759 196
Fragments arranged by size:
759 196 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 245 1140
Size: 245 895
BtsI GCAGTG_nn'
Cuts at: 0 191 226 1001 1140
Size: 191 35 775 139
Fragments arranged by size:
775 191 139 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 160 1140
Size: 160 980
EaeI y'GGCC_r
Cuts at: 0 65 158 947 1140
Size: 65 93 789 193
Fragments arranged by size:
789 193 93 65
EagI C'GGCC_G
Cuts at: 0 65 1140
Size: 65 1075
EarI CTCTTCn'nnn_
Cuts at: 0 404 635 1140
Size: 404 231 505
Fragments arranged by size:
505 404 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 150 303 383 580 811 979 1140
Size: 96 54 153 80 197 231 168 161
Fragments arranged by size:
231 197 168 161 153 96 80 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 559 682 1140
Size: 559 123 458
Fragments arranged by size:
559 458 123
EcoO109I rG'GnC_Cy
Cuts at: 0 675 887 940 1140
Size: 675 212 53 200
Fragments arranged by size:
675 212 200 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 64 94 96 126 511 543
Size: 22 32 10 30 2 30 385 32
Cuts at: 543 1140
Size: 597
Fragments arranged by size:
597 385 32 32 30 30 22 10
2
HincII GTy'rAC
Cuts at: 0 132 1140
Size: 132 1008
MscI TGG'CCA
Cuts at: 0 949 1140
Size: 949 191
MslI CAynn'nnrTG
Cuts at: 0 976 1140
Size: 976 164
MspA1I CmG'CkG
Cuts at: 0 47 307 998 1058 1066 1140
Size: 47 260 691 60 8 74
Fragments arranged by size:
691 260 74 60 47 8
NcoI C'CATG_G
Cuts at: 0 944 1140
Size: 944 196
PasI CC'CwG_GG
Cuts at: 0 930 1140
Size: 930 210
PpuMI rG'GwC_Cy
Cuts at: 0 887 1140
Size: 887 253
PspOMI G'GGCC_C
Cuts at: 0 940 1140
Size: 940 200
PstI C_TGCA'G
Cuts at: 0 52 221 453 1140
Size: 52 169 232 687
Fragments arranged by size:
687 232 169 52
PvuII CAG'CTG
Cuts at: 0 998 1058 1066 1140
Size: 998 60 8 74
Fragments arranged by size:
998 74 60 8
SfcI C'TryA_G
Cuts at: 0 48 217 449 1140
Size: 48 169 232 691
Fragments arranged by size:
691 232 169 48
StuI AGG'CCT
Cuts at: 0 712 1140
Size: 712 428
StyI C'CwwG_G
Cuts at: 0 944 1140
Size: 944 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 811 1137 1140
Size: 556 255 326 3
Fragments arranged by size:
556 326 255 3
TatI w'GTAC_w
Cuts at: 0 988 1140
Size: 988 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 910 942 952 975 984 1007
Size: 205 32 673 32 10 23 9 23
Cuts at: 1007 1140
Size: 133
Fragments arranged by size:
673 205 133 32 32 23 23 10
9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 753 1140
Size: 753 387
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI
BanII BbsI BcgI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BstEII BstXI BstYI BtgI BtgZI BtsI DrdI EaeI
EagI EarI Eco57MI EcoNI EcoO109I Hin4I HincII MscI
MslI MspA1I NcoI PasI PpuMI PspOMI PstI PvuII
SfcI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BmtI BpuEI BsaI BsaAI BsaBI BsiWI BsmI
BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI