(Linear) (Six-base) MAPSORT of: KIBB9577.seq Check: 5501 from: 1 to: 3633 KIBB9577.orf With 182 enzymes: SgfI * March 3, 2008 11:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1735 2497 3204 3633 Size: 1735 762 707 429 Fragments arranged by size: 1735 762 707 429 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 3633 Size: 814 2819 AflII C'TTAA_G Cuts at: 0 1674 3633 Size: 1674 1959 AflIII A'CryG_T Cuts at: 0 1577 1944 2645 2801 3633 Size: 1577 367 701 156 832 Fragments arranged by size: 1577 832 701 367 156 AgeI A'CCGG_T Cuts at: 0 2915 3633 Size: 2915 718 AleI CACnn'nnGTG Cuts at: 0 520 1958 2644 2995 3633 Size: 520 1438 686 351 638 Fragments arranged by size: 1438 686 638 520 351 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1934 1966 2902 2934 3633 Size: 1934 32 936 32 699 Fragments arranged by size: 1934 936 699 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1195 3129 3546 3633 Size: 1195 1934 417 87 Fragments arranged by size: 1934 1195 417 87 ApoI r'AATT_y Cuts at: 0 1023 1855 1894 2186 3230 3633 Size: 1023 832 39 292 1044 403 Fragments arranged by size: 1044 1023 832 403 292 39 AseI AT'TA_AT Cuts at: 0 1043 1847 3633 Size: 1043 804 1786 Fragments arranged by size: 1786 1043 804 AvaI C'yCGr_G Cuts at: 0 82 350 2971 3633 Size: 82 268 2621 662 Fragments arranged by size: 2621 662 268 82 AvrII C'CTAG_G Cuts at: 0 55 3633 Size: 55 3578 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2821 2854 3283 3316 3633 Size: 2821 33 429 33 317 Fragments arranged by size: 2821 429 317 33 33 BamHI G'GATC_C Cuts at: 0 485 3633 Size: 485 3148 BanI G'GyrC_C Cuts at: 0 2148 3591 3633 Size: 2148 1443 42 Fragments arranged by size: 2148 1443 42 BanII G_rGCy'C Cuts at: 0 83 89 452 1283 3629 3633 Size: 83 6 363 831 2346 4 Fragments arranged by size: 2346 831 363 83 6 4 BbsI GAAGACnn'nnnn_ Cuts at: 0 314 2552 3633 Size: 314 2238 1081 Fragments arranged by size: 2238 1081 314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2962 2996 3633 Size: 2962 34 637 Fragments arranged by size: 2962 637 34 BglI GCCn_nnn'nGGC Cuts at: 0 1206 2949 3633 Size: 1206 1743 684 Fragments arranged by size: 1743 1206 684 BlpI GC'TnA_GC Cuts at: 0 4 2096 3633 Size: 4 2092 1537 Fragments arranged by size: 2092 1537 4 Bme1580I G_kGCm'C Cuts at: 0 988 2636 3633 Size: 988 1648 997 Fragments arranged by size: 1648 997 988 BmgBI CAC'GTC Cuts at: 0 663 1947 3633 Size: 663 1284 1686 Fragments arranged by size: 1686 1284 663 BmrI ACTGGGnnnn_n' Cuts at: 0 474 2221 2755 3580 3633 Size: 474 1747 534 825 53 Fragments arranged by size: 1747 825 534 474 53 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1751 3633 Size: 1751 1882 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 97 165 445 729 1106 1202 1723 Size: 57 40 68 280 284 377 96 521 Cuts at: 1723 3147 3633 Size: 1424 486 Fragments arranged by size: 1424 521 486 377 284 280 96 68 57 40 Bpu10I CC'TnA_GC Cuts at: 0 1196 2598 3633 Size: 1196 1402 1035 Fragments arranged by size: 1402 1196 1035 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1443 1639 1754 3633 Size: 1443 196 115 1879 Fragments arranged by size: 1879 1443 196 115 BsaI GGTCTCn'nnnn_ Cuts at: 0 2735 3455 3633 Size: 2735 720 178 Fragments arranged by size: 2735 720 178 BsaAI yAC'GTr Cuts at: 0 500 1809 2646 3633 Size: 500 1309 837 987 Fragments arranged by size: 1309 987 837 500 BsaBI GATnn'nnATC Cuts at: 0 2578 3633 Size: 2578 1055 BsaHI Gr'CG_yC Cuts at: 0 746 2516 3191 3633 Size: 746 1770 675 442 Fragments arranged by size: 1770 746 675 442 BsaWI w'CCGG_w Cuts at: 0 482 2570 2915 3206 3633 Size: 482 2088 345 291 427 Fragments arranged by size: 2088 482 427 345 291 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 418 448 452 463 482 493 2612 2642 Size: 418 30 4 11 19 11 2119 30 Cuts at: 2642 3145 3175 3633 Size: 503 30 458 Fragments arranged by size: 2119 503 458 418 30 30 30 19 11 11 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1363 1636 2125 2515 3295 3633 Size: 1363 273 489 390 780 338 Fragments arranged by size: 1363 780 489 390 338 273 BseYI C'CCAG_C Cuts at: 0 932 2662 3009 3014 3435 3628 3633 Size: 932 1730 347 5 421 193 5 Fragments arranged by size: 1730 932 421 347 193 5 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2422 3518 3633 Size: 2422 1096 115 Fragments arranged by size: 2422 1096 115 BsiEI CG_ry'CG Cuts at: 0 3113 3323 3633 Size: 3113 210 310 Fragments arranged by size: 3113 310 210 BsiHKAI G_wGCw'C Cuts at: 0 89 1283 3633 Size: 89 1194 2350 Fragments arranged by size: 2350 1194 89 BsiWI C'GTAC_G Cuts at: 0 3324 3633 Size: 3324 309 BsmI GAATG_Cn' Cuts at: 0 2689 3633 Size: 2689 944 BsmBI CGTCTCn'nnnn_ Cuts at: 0 737 2531 3633 Size: 737 1794 1102 Fragments arranged by size: 1794 1102 737 Bsp1286I G_dGCh'C Cuts at: 0 83 89 452 988 1283 2636 3629 3633 Size: 83 6 363 536 295 1353 993 4 Fragments arranged by size: 1353 993 536 363 295 83 6 4 BspEI T'CCGG_A Cuts at: 0 2570 3633 Size: 2570 1063 BspHI T'CATG_A Cuts at: 0 759 1821 3633 Size: 759 1062 1812 Fragments arranged by size: 1812 1062 759 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1735 2144 2483 2497 3204 3633 Size: 1735 409 339 14 707 429 Fragments arranged by size: 1735 707 429 409 339 14 BsrBI CCG'CTC Cuts at: 0 1664 3633 Size: 1664 1969 BsrFI r'CCGG_y Cuts at: 0 2139 2460 2915 3633 Size: 2139 321 455 718 Fragments arranged by size: 2139 718 455 321 BssHII G'CGCG_C Cuts at: 0 1366 3633 Size: 1366 2267 BssSI C'ACGA_G Cuts at: 0 2422 2440 2923 3633 Size: 2422 18 483 710 Fragments arranged by size: 2422 710 483 18 BstAPI GCAn_nnn'nTGC Cuts at: 0 3546 3633 Size: 3546 87 BstEII G'GTnAC_C Cuts at: 0 2997 3633 Size: 2997 636 BstXI CCAn_nnnn'nTGG Cuts at: 0 24 1382 3633 Size: 24 1358 2251 Fragments arranged by size: 2251 1358 24 BstYI r'GATC_y Cuts at: 0 485 1689 3633 Size: 485 1204 1944 Fragments arranged by size: 1944 1204 485 BtgI C'CryG_G Cuts at: 0 324 866 989 2468 2783 3054 3633 Size: 324 542 123 1479 315 271 579 Fragments arranged by size: 1479 579 542 324 315 271 123 BtsI GCAGTG_nn' Cuts at: 0 680 2052 3535 3633 Size: 680 1372 1483 98 Fragments arranged by size: 1483 1372 680 98 ClaI AT'CG_AT Cuts at: 0 3357 3633 Size: 3357 276 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2028 2063 3633 Size: 2028 35 1570 Fragments arranged by size: 2028 1570 35 DraI TTT'AAA Cuts at: 0 1763 3633 Size: 1763 1870 DrdI GACnn_nn'nnGTC Cuts at: 0 3190 3327 3633 Size: 3190 137 306 Fragments arranged by size: 3190 306 137 EaeI y'GGCC_r Cuts at: 0 1266 1718 2462 2721 3110 3633 Size: 1266 452 744 259 389 523 Fragments arranged by size: 1266 744 523 452 389 259 EagI C'GGCC_G Cuts at: 0 3110 3633 Size: 3110 523 EarI CTCTTCn'nnn_ Cuts at: 0 730 3633 Size: 730 2903 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 61 3633 Size: 61 3572 EcoICRI GAG'CTC Cuts at: 0 87 1281 3633 Size: 87 1194 2352 Fragments arranged by size: 2352 1194 87 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 97 165 445 729 814 1106 1202 Size: 57 40 68 280 284 85 292 96 Cuts at: 1202 1723 3147 3633 Size: 521 1424 486 Fragments arranged by size: 1424 521 486 292 284 280 96 85 68 57 40 EcoNI CCTnn'n_nnAGG Cuts at: 0 382 3032 3633 Size: 382 2650 601 Fragments arranged by size: 2650 601 382 EcoO109I rG'GnC_Cy Cuts at: 0 1707 2133 2928 3126 3633 Size: 1707 426 795 198 507 Fragments arranged by size: 1707 795 507 426 198 EcoRI G'AATT_C Cuts at: 0 1023 3230 3633 Size: 1023 2207 403 Fragments arranged by size: 2207 1023 403 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3054 3633 Size: 3054 579 HaeII r_GCGC'y Cuts at: 0 718 3633 Size: 718 2915 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1012 1044 1775 1807 2534 2566 3633 Size: 1012 32 731 32 727 32 1067 Fragments arranged by size: 1067 1012 731 727 32 32 32 HincII GTy'rAC Cuts at: 0 1412 3633 Size: 1412 2221 HindIII A'AGCT_T Cuts at: 0 1390 3419 3633 Size: 1390 2029 214 Fragments arranged by size: 2029 1390 214 HpaI GTT'AAC Cuts at: 0 1412 3633 Size: 1412 2221 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 198 1067 2362 2429 2690 3249 3404 3633 Size: 198 869 1295 67 261 559 155 229 Fragments arranged by size: 1295 869 559 261 229 198 155 67 MscI TGG'CCA Cuts at: 0 2723 3633 Size: 2723 910 MslI CAynn'nnrTG Cuts at: 0 520 776 1958 2644 2995 3633 Size: 520 256 1182 686 351 638 Fragments arranged by size: 1182 686 638 520 351 256 MspA1I CmG'CkG Cuts at: 0 75 917 1271 3633 Size: 75 842 354 2362 Fragments arranged by size: 2362 842 354 75 NaeI GCC'GGC Cuts at: 0 2462 3633 Size: 2462 1171 NcoI C'CATG_G Cuts at: 0 324 866 2468 2783 3054 3633 Size: 324 542 1602 315 271 579 Fragments arranged by size: 1602 579 542 324 315 271 NgoMIV G'CCGG_C Cuts at: 0 2460 3633 Size: 2460 1173 NspI r_CATG'y Cuts at: 0 1581 1789 2591 3633 Size: 1581 208 802 1042 Fragments arranged by size: 1581 1042 802 208 PasI CC'CwG_GG Cuts at: 0 1711 3633 Size: 1711 1922 PciI A'CATG_T Cuts at: 0 1577 3633 Size: 1577 2056 PflMI CCAn_nnn'nTGG Cuts at: 0 966 3633 Size: 966 2667 PmlI CAC'GTG Cuts at: 0 500 2646 3633 Size: 500 2146 987 Fragments arranged by size: 2146 987 500 PpuMI rG'GwC_Cy Cuts at: 0 1707 2133 3126 3633 Size: 1707 426 993 507 Fragments arranged by size: 1707 993 507 426 PshAI GACnn'nnGTC Cuts at: 0 2282 3633 Size: 2282 1351 PspXI vC'TCGA_Gb Cuts at: 0 2971 3633 Size: 2971 662 PstI C_TGCA'G Cuts at: 0 145 2163 2494 3542 3633 Size: 145 2018 331 1048 91 Fragments arranged by size: 2018 1048 331 145 91 PvuII CAG'CTG Cuts at: 0 75 917 3633 Size: 75 842 2716 Fragments arranged by size: 2716 842 75 SacI G_AGCT'C Cuts at: 0 89 1283 3633 Size: 89 1194 2350 Fragments arranged by size: 2350 1194 89 SbfI CC_TGCA'GG Cuts at: 0 2494 3633 Size: 2494 1139 SfcI C'TryA_G Cuts at: 0 141 1427 2159 2455 2490 3538 3633 Size: 141 1286 732 296 35 1048 95 Fragments arranged by size: 1286 1048 732 296 141 95 35 SmlI C'TyrA_G Cuts at: 0 1458 1618 1674 1769 2971 3633 Size: 1458 160 56 95 1202 662 Fragments arranged by size: 1458 1202 662 160 95 56 SspI AAT'ATT Cuts at: 0 1041 3633 Size: 1041 2592 StuI AGG'CCT Cuts at: 0 1731 3633 Size: 1731 1902 StyI C'CwwG_G Cuts at: 0 55 324 614 866 2468 2783 3054 3633 Size: 55 269 290 252 1602 315 271 579 Fragments arranged by size: 1602 579 315 290 271 269 252 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 456 3633 Size: 456 3177 TatI w'GTAC_w Cuts at: 0 1775 3633 Size: 1775 1858 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 212 244 418 450 452 484 2256 2288 Size: 212 32 174 32 2 32 1772 32 Cuts at: 2288 2315 2347 3145 3177 3633 Size: 27 32 798 32 456 Fragments arranged by size: 1772 798 456 212 174 32 32 32 32 32 32 27 2 Tth111I GACn'n_nGTC Cuts at: 0 553 664 1925 2518 3633 Size: 553 111 1261 593 1115 Fragments arranged by size: 1261 1115 593 553 111 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2098 2781 3633 Size: 2098 683 852 Fragments arranged by size: 2098 852 683 XhoI C'TCGA_G Cuts at: 0 2971 3633 Size: 2971 662 XmnI GAAnn'nnTTC Cuts at: 0 3037 3633 Size: 3037 596 Enzymes that do cut: AarI AcuI AflII AflIII AgeI AleI AloI AlwNI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PmlI PpuMI PshAI PspXI PstI PvuII SacI SbfI SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AhdI AlfI ApaI ApaLI AscI AsiSI BbeI BbvCI BciVI BclI BfrBI BglII BmtI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI DraIII EcoRV FseI FspI FspAI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PpiI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XbaI XmaI ZraI