(Linear) (Six-base) MAPSORT of: KIBB9580.seq  Check: 8805  from: 1  to: 543



KIBB9580.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AccI GT'mk_AC

Cuts at:      0      27     543 
   Size:         27     516
 
ApaLI G'TGCA_C

Cuts at:      0     115     543 
   Size:        115     428
 
BanII G_rGCy'C

Cuts at:      0      98     543 
   Size:         98     445
 
Bme1580I G_kGCm'C

Cuts at:      0     119     543 
   Size:        119     424
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     543 
   Size:        112     431
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     457     543 
   Size:        457      86
 
BsaAI yAC'GTr

Cuts at:      0     114     543 
   Size:        114     429
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     166     379     543 
   Size:        166     213     164

  Fragments arranged by size: 

                 213     166     164
 
BseYI C'CCAG_C

Cuts at:      0     175     341     543 
   Size:        175     166     202

  Fragments arranged by size: 

                 202     175     166
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     543 
   Size:        119     424
 
Bsp1286I G_dGCh'C

Cuts at:      0      98     119     543 
   Size:         98      21     424

  Fragments arranged by size: 

                 424      98      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     440     543 
   Size:        440     103
 
BsrBI CCG'CTC

Cuts at:      0     300     543 
   Size:        300     243
 
BtgI C'CryG_G

Cuts at:      0      98     160     543 
   Size:         98      62     383

  Fragments arranged by size: 

                 383      98      62
 
EarI CTCTTCn'nnn_

Cuts at:      0     446     543 
   Size:        446      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     337     543 
   Size:        337     206
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     543 
   Size:        112     431
 
EcoO109I rG'GnC_Cy

Cuts at:      0      80     126     543 
   Size:         80      46     417

  Fragments arranged by size: 

                 417      80      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     222     254     398     430     543 
   Size:        222      32     144      32     113

  Fragments arranged by size: 

                 222     144     113      32      32
 
MspA1I CmG'CkG

Cuts at:      0     312     543 
   Size:        312     231
 
NcoI C'CATG_G

Cuts at:      0      98     543 
   Size:         98     445
 
PmlI CAC'GTG

Cuts at:      0     114     543 
   Size:        114     429
 
PpuMI rG'GwC_Cy

Cuts at:      0      80     543 
   Size:         80     463
 
PstI C_TGCA'G

Cuts at:      0     437     543 
   Size:        437     106
 
ScaI AGT'ACT

Cuts at:      0     262     543 
   Size:        262     281
 
SexAI A'CCwGG_T

Cuts at:      0      76     543 
   Size:         76     467
 
SfcI C'TryA_G

Cuts at:      0     433     543 
   Size:        433     110
 
StyI C'CwwG_G

Cuts at:      0      98     543 
   Size:         98     445
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     134     543 
   Size:        134     409
 
TatI w'GTAC_w

Cuts at:      0     260     419     543 
   Size:        260     159     124

  Fragments arranged by size: 

                 260     159     124
 
Tth111I GACn'n_nGTC

Cuts at:      0      78     543 
   Size:         78     465
 
XmnI GAAnn'nnTTC

Cuts at:      0     486     543 
   Size:        486      57
 

 Enzymes that do cut:

     AccI    ApaLI    BanII Bme1580I     BpmI     BsaI    BsaAI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspMI    BsrBI     BtgI     EarI     EciI
  Eco57MI EcoO109I    Hin4I   MspA1I     NcoI     PmlI    PpuMI     PstI
     ScaI    SexAI     SfcI     StyI    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI