Restriction map for
pF1KB9584
(Linear) (Six-base) MAPSORT of: KIBB9584.seq Check: 3835 from: 1 to: 810
KIBB9584.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 437 810
Size: 361 76 373
Fragments arranged by size:
373 361 76
AvaI C'yCGr_G
Cuts at: 0 99 249 507 546 810
Size: 99 150 258 39 264
Fragments arranged by size:
264 258 150 99 39
BanI G'GyrC_C
Cuts at: 0 46 242 810
Size: 46 196 568
Fragments arranged by size:
568 196 46
BanII G_rGCy'C
Cuts at: 0 106 810
Size: 106 704
BglI GCCn_nnn'nGGC
Cuts at: 0 268 320 613 810
Size: 268 52 293 197
Fragments arranged by size:
293 268 197 52
Bme1580I G_kGCm'C
Cuts at: 0 245 810
Size: 245 565
BmgBI CAC'GTC
Cuts at: 0 621 810
Size: 621 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 282 810
Size: 282 528
BseYI C'CCAG_C
Cuts at: 0 131 741 793 810
Size: 131 610 52 17
Fragments arranged by size:
610 131 52 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 810
Size: 43 767
BsiHKAI G_wGCw'C
Cuts at: 0 164 810
Size: 164 646
BsmI GAATG_Cn'
Cuts at: 0 195 810
Size: 195 615
Bsp1286I G_dGCh'C
Cuts at: 0 106 164 245 810
Size: 106 58 81 565
Fragments arranged by size:
565 106 81 58
BsrBI CCG'CTC
Cuts at: 0 200 810
Size: 200 610
BsrDI GCAATG_nn'
Cuts at: 0 638 810
Size: 638 172
BsrFI r'CCGG_y
Cuts at: 0 330 810
Size: 330 480
BssHII G'CGCG_C
Cuts at: 0 254 810
Size: 254 556
BssSI C'ACGA_G
Cuts at: 0 195 810
Size: 195 615
BtgI C'CryG_G
Cuts at: 0 27 266 611 810
Size: 27 239 345 199
Fragments arranged by size:
345 239 199 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 229 810
Size: 229 581
EaeI y'GGCC_r
Cuts at: 0 17 269 308 332 810
Size: 17 252 39 24 478
Fragments arranged by size:
478 252 39 24 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 422 810
Size: 422 388
EcoO109I rG'GnC_Cy
Cuts at: 0 298 810
Size: 298 512
HaeII r_GCGC'y
Cuts at: 0 36 810
Size: 36 774
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 810
Size: 93 32 685
Fragments arranged by size:
685 93 32
MscI TGG'CCA
Cuts at: 0 19 310 810
Size: 19 291 500
Fragments arranged by size:
500 291 19
MslI CAynn'nnrTG
Cuts at: 0 85 810
Size: 85 725
MspA1I CmG'CkG
Cuts at: 0 268 345 482 575 587 593 628 810
Size: 268 77 137 93 12 6 35 182
Fragments arranged by size:
268 182 137 93 77 35 12 6
NaeI GCC'GGC
Cuts at: 0 332 810
Size: 332 478
NdeI CA'TA_TG
Cuts at: 0 11 810
Size: 11 799
NgoMIV G'CCGG_C
Cuts at: 0 330 810
Size: 330 480
NspI r_CATG'y
Cuts at: 0 214 342 405 603 810
Size: 214 128 63 198 207
Fragments arranged by size:
214 207 198 128 63
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 81 294 810
Size: 15 66 213 516
Fragments arranged by size:
516 213 66 15
PpuMI rG'GwC_Cy
Cuts at: 0 298 810
Size: 298 512
PstI C_TGCA'G
Cuts at: 0 350 426 810
Size: 350 76 384
Fragments arranged by size:
384 350 76
PvuII CAG'CTG
Cuts at: 0 345 810
Size: 345 465
SacII CC_GC'GG
Cuts at: 0 269 810
Size: 269 541
SanDI GG'GwC_CC
Cuts at: 0 298 810
Size: 298 512
SfcI C'TryA_G
Cuts at: 0 346 422 810
Size: 346 76 388
Fragments arranged by size:
388 346 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 268 810
Size: 268 542
SphI G_CATG'C
Cuts at: 0 214 342 810
Size: 214 128 468
Fragments arranged by size:
468 214 128
StyI C'CwwG_G
Cuts at: 0 689 810
Size: 689 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 810
Size: 763 32 15
Fragments arranged by size:
763 32 15
Enzymes that do cut:
AlfI AvaI BanI BanII BglI Bme1580I BmgBI BmrI
BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI
BssHII BssSI BtgI BtgZI EaeI EcoNI EcoO109I HaeII
Hin4I MscI MslI MspA1I NaeI NdeI NgoMIV NspI
PflMI PpuMI PstI PvuII SacII SanDI SfcI SfiI
SphI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrGI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NcoI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI