Restriction map for
pF1KB9587
(Linear) (Six-base) MAPSORT of: KIBB9587.seq Check: 1565 from: 1 to: 708
KIBB9587.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 708
Size: 288 420
AccI GT'mk_AC
Cuts at: 0 62 492 708
Size: 62 430 216
Fragments arranged by size:
430 216 62
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 708
Size: 37 671
AleI CACnn'nnGTG
Cuts at: 0 94 708
Size: 94 614
AlwNI CAG_nnn'CTG
Cuts at: 0 484 571 592 681 708
Size: 484 87 21 89 27
Fragments arranged by size:
484 89 87 27 21
ApaI G_GGCC'C
Cuts at: 0 478 708
Size: 478 230
BanI G'GyrC_C
Cuts at: 0 105 194 243 457 561 708
Size: 105 89 49 214 104 147
Fragments arranged by size:
214 147 105 104 89 49
BanII G_rGCy'C
Cuts at: 0 478 708
Size: 478 230
BbvCI CC'TCA_GC
Cuts at: 0 364 708
Size: 364 344
BglI GCCn_nnn'nGGC
Cuts at: 0 343 708
Size: 343 365
BlpI GC'TnA_GC
Cuts at: 0 4 708
Size: 4 704
Bme1580I G_kGCm'C
Cuts at: 0 478 564 673 708
Size: 478 86 109 35
Fragments arranged by size:
478 109 86 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 284 708
Size: 284 424
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 407 708
Size: 407 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 703 708
Size: 376 327 5
Fragments arranged by size:
376 327 5
Bpu10I CC'TnA_GC
Cuts at: 0 364 708
Size: 364 344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 529 708
Size: 136 393 179
Fragments arranged by size:
393 179 136
BsaHI Gr'CG_yC
Cuts at: 0 235 708
Size: 235 473
BsaWI w'CCGG_w
Cuts at: 0 621 708
Size: 621 87
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 49 52 55 708
Size: 46 3 3 3 653
Fragments arranged by size:
653 46 3 3 3
BseYI C'CCAG_C
Cuts at: 0 448 471 477 708
Size: 448 23 6 231
Fragments arranged by size:
448 231 23 6
BsiEI CG_ry'CG
Cuts at: 0 116 691 708
Size: 116 575 17
Fragments arranged by size:
575 116 17
BsiHKAI G_wGCw'C
Cuts at: 0 152 708
Size: 152 556
Bsp1286I G_dGCh'C
Cuts at: 0 152 478 564 673 708
Size: 152 326 86 109 35
Fragments arranged by size:
326 152 109 86 35
BspEI T'CCGG_A
Cuts at: 0 621 708
Size: 621 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 197 288 476 708
Size: 197 91 188 232
Fragments arranged by size:
232 197 188 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 708
Size: 201 507
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 708
Size: 354 354
BstYI r'GATC_y
Cuts at: 0 78 708
Size: 78 630
Bsu36I CC'TnA_GG
Cuts at: 0 593 708
Size: 593 115
BtgI C'CryG_G
Cuts at: 0 116 239 248 341 708
Size: 116 123 9 93 367
Fragments arranged by size:
367 123 116 93 9
DraIII CAC_nnn'GTG
Cuts at: 0 201 708
Size: 201 507
EaeI y'GGCC_r
Cuts at: 0 84 113 344 708
Size: 84 29 231 364
Fragments arranged by size:
364 231 84 29
EagI C'GGCC_G
Cuts at: 0 113 708
Size: 113 595
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 376 703 708
Size: 37 339 327 5
Fragments arranged by size:
339 327 37 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 385 520 708
Size: 355 30 135 188
Fragments arranged by size:
355 188 135 30
EcoO109I rG'GnC_Cy
Cuts at: 0 360 378 513 576 589 708
Size: 360 18 135 63 13 119
Fragments arranged by size:
360 135 119 63 18 13
HaeII r_GCGC'y
Cuts at: 0 448 708
Size: 448 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 708
Size: 29 679
HincII GTy'rAC
Cuts at: 0 63 708
Size: 63 645
MscI TGG'CCA
Cuts at: 0 86 346 708
Size: 86 260 362
Fragments arranged by size:
362 260 86
MslI CAynn'nnrTG
Cuts at: 0 94 538 658 708
Size: 94 444 120 50
Fragments arranged by size:
444 120 94 50
MspA1I CmG'CkG
Cuts at: 0 94 250 708
Size: 94 156 458
Fragments arranged by size:
458 156 94
NcoI C'CATG_G
Cuts at: 0 239 341 708
Size: 239 102 367
Fragments arranged by size:
367 239 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 402 484 708
Size: 402 82 224
Fragments arranged by size:
402 224 82
PfoI T'CCnGG_A
Cuts at: 0 353 708
Size: 353 355
PpuMI rG'GwC_Cy
Cuts at: 0 360 708
Size: 360 348
PspOMI G'GGCC_C
Cuts at: 0 474 708
Size: 474 234
PstI C_TGCA'G
Cuts at: 0 173 285 708
Size: 173 112 423
Fragments arranged by size:
423 173 112
SacII CC_GC'GG
Cuts at: 0 251 708
Size: 251 457
SalI G'TCGA_C
Cuts at: 0 61 708
Size: 61 647
SexAI A'CCwGG_T
Cuts at: 0 197 708
Size: 197 511
SfcI C'TryA_G
Cuts at: 0 169 281 708
Size: 169 112 427
Fragments arranged by size:
427 169 112
SmlI C'TyrA_G
Cuts at: 0 151 508 708
Size: 151 357 200
Fragments arranged by size:
357 200 151
StyI C'CwwG_G
Cuts at: 0 239 341 401 461 708
Size: 239 102 60 60 247
Fragments arranged by size:
247 239 102 60 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 708
Size: 445 263
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI BanI BanII
BbvCI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BstAPI BstXI BstYI Bsu36I BtgI DraIII
EaeI EagI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
MscI MslI MspA1I NcoI PflMI PfoI PpuMI PspOMI
PstI SacII SalI SexAI SfcI SmlI StyI TaqII
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI BsaI BsaAI BsaBI BsaXI
BsgI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI
ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI