Restriction map for
pF1KB9592
(Linear) (Six-base) MAPSORT of: KIBB9592.seq Check: 6906 from: 1 to: 1779
KIBB9592.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 0 1388 1548 1779
Size: 1388 160 231
Fragments arranged by size:
1388 231 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1779
Size: 982 797
AflIII A'CryG_T
Cuts at: 0 1551 1746 1779
Size: 1551 195 33
Fragments arranged by size:
1551 195 33
AgeI A'CCGG_T
Cuts at: 0 659 1779
Size: 659 1120
AhdI GACnn_n'nnGTC
Cuts at: 0 1580 1779
Size: 1580 199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 197 1779
Size: 197 1582
AlwNI CAG_nnn'CTG
Cuts at: 0 106 1320 1597 1779
Size: 106 1214 277 182
Fragments arranged by size:
1214 277 182 106
ApoI r'AATT_y
Cuts at: 0 326 431 535 705 724 775 811 1779
Size: 326 105 104 170 19 51 36 968
Fragments arranged by size:
968 326 170 105 104 51 36 19
BamHI G'GATC_C
Cuts at: 0 711 1779
Size: 711 1068
BanI G'GyrC_C
Cuts at: 0 898 1779
Size: 898 881
BbsI GAAGACnn'nnnn_
Cuts at: 0 1617 1779
Size: 1617 162
BciVI GTATCCnnnnn_n'
Cuts at: 0 1291 1779
Size: 1291 488
BclI T'GATC_A
Cuts at: 0 1355 1779
Size: 1355 424
BfrBI ATG'CAT
Cuts at: 0 770 1779
Size: 770 1009
BmgBI CAC'GTC
Cuts at: 0 655 1552 1779
Size: 655 897 227
Fragments arranged by size:
897 655 227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 237 1779
Size: 237 1542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1558 1574 1591 1779
Size: 1558 16 17 188
Fragments arranged by size:
1558 188 17 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 1567 1779
Size: 1567 212
BsaBI GATnn'nnATC
Cuts at: 0 1415 1779
Size: 1415 364
BsaWI w'CCGG_w
Cuts at: 0 659 1779
Size: 659 1120
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 324 1779
Size: 324 1455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1779
Size: 307 1472
BsmBI CGTCTCn'nnnn_
Cuts at: 0 645 1779
Size: 645 1134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 364 1779
Size: 364 1415
BsrFI r'CCGG_y
Cuts at: 0 659 1779
Size: 659 1120
BsrGI T'GTAC_A
Cuts at: 0 360 510 1779
Size: 360 150 1269
Fragments arranged by size:
1269 360 150
BstBI TT'CG_AA
Cuts at: 0 1472 1779
Size: 1472 307
BstXI CCAn_nnnn'nTGG
Cuts at: 0 909 1779
Size: 909 870
BstYI r'GATC_y
Cuts at: 0 711 1779
Size: 711 1068
BtgI C'CryG_G
Cuts at: 0 1431 1578 1779
Size: 1431 147 201
Fragments arranged by size:
1431 201 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1701 1779
Size: 1701 78
DraI TTT'AAA
Cuts at: 0 1172 1779
Size: 1172 607
EaeI y'GGCC_r
Cuts at: 0 1599 1779
Size: 1599 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1558 1574 1591 1779
Size: 982 576 16 17 188
Fragments arranged by size:
982 576 188 17 16
EcoRI G'AATT_C
Cuts at: 0 535 811 1779
Size: 535 276 968
Fragments arranged by size:
968 535 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 199 217 231 249 1116 1148
Size: 106 32 61 18 14 18 867 32
Cuts at: 1148 1566 1598 1779
Size: 418 32 181
Fragments arranged by size:
867 418 181 106 61 32 32 32
18 18 14
HincII GTy'rAC
Cuts at: 0 1549 1779
Size: 1549 230
MfeI C'AATT_G
Cuts at: 0 1498 1779
Size: 1498 281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 1779
Size: 509 1270
MscI TGG'CCA
Cuts at: 0 1601 1779
Size: 1601 178
MslI CAynn'nnrTG
Cuts at: 0 412 1779
Size: 412 1367
MspA1I CmG'CkG
Cuts at: 0 181 1779
Size: 181 1598
NcoI C'CATG_G
Cuts at: 0 1578 1779
Size: 1578 201
NdeI CA'TA_TG
Cuts at: 0 1512 1779
Size: 1512 267
NsiI A_TGCA'T
Cuts at: 0 772 1779
Size: 772 1007
NspI r_CATG'y
Cuts at: 0 691 1750 1779
Size: 691 1059 29
Fragments arranged by size:
1059 691 29
PciI A'CATG_T
Cuts at: 0 1746 1779
Size: 1746 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 1377 1569 1597 1779
Size: 1377 192 28 182
Fragments arranged by size:
1377 192 182 28
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1357 1389 1779
Size: 1357 32 390
Fragments arranged by size:
1357 390 32
PstI C_TGCA'G
Cuts at: 0 1234 1779
Size: 1234 545
PvuII CAG'CTG
Cuts at: 0 181 1779
Size: 181 1598
SalI G'TCGA_C
Cuts at: 0 1547 1779
Size: 1547 232
ScaI AGT'ACT
Cuts at: 0 481 1779
Size: 481 1298
SfcI C'TryA_G
Cuts at: 0 71 98 1230 1779
Size: 71 27 1132 549
Fragments arranged by size:
1132 549 71 27
SspI AAT'ATT
Cuts at: 0 1224 1779
Size: 1224 555
StyI C'CwwG_G
Cuts at: 0 1578 1779
Size: 1578 201
TatI w'GTAC_w
Cuts at: 0 360 479 510 630 1779
Size: 360 119 31 120 1149
Fragments arranged by size:
1149 360 120 119 31
Tth111I GACn'n_nGTC
Cuts at: 0 457 1779
Size: 457 1322
XmnI GAAnn'nnTTC
Cuts at: 0 443 1779
Size: 443 1336
Enzymes that do cut:
AccI AcuI AflIII AgeI AhdI AlfI AlwNI ApoI
BamHI BanI BbsI BciVI BclI BfrBI BmgBI BplI
BpmI BsaI BsaBI BsaWI BseRI BsgI BsmBI BspMI
BsrFI BsrGI BstBI BstXI BstYI BtgI BtgZI DraI
EaeI Eco57MI EcoRI Hin4I HincII MfeI MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI
PsrI PstI PvuII SalI ScaI SfcI SspI StyI
TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I
BmrI BmtI Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BsrBI
BsrDI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtsI
ClaI CspCI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TstI XbaI XcmI XhoI XmaI
ZraI