Restriction map for
pF1KB9603
(Linear) (Six-base) MAPSORT of: KIBB9603.seq Check: 7423 from: 1 to: 1035
KIBB9603.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 812 1035
Size: 765 47 223
Fragments arranged by size:
765 223 47
AflIII A'CryG_T
Cuts at: 0 392 1035
Size: 392 643
AlwNI CAG_nnn'CTG
Cuts at: 0 365 1035
Size: 365 670
ApaI G_GGCC'C
Cuts at: 0 445 453 541 577 866 1035
Size: 445 8 88 36 289 169
Fragments arranged by size:
445 289 169 88 36 8
AvaI C'yCGr_G
Cuts at: 0 67 120 196 445 534 717 973 1035
Size: 67 53 76 249 89 183 256 62
Fragments arranged by size:
256 249 183 89 76 67 62 53
AvrII C'CTAG_G
Cuts at: 0 843 1035
Size: 843 192
BamHI G'GATC_C
Cuts at: 0 977 1035
Size: 977 58
BanI G'GyrC_C
Cuts at: 0 327 416 609 850 880 1035
Size: 327 89 193 241 30 155
Fragments arranged by size:
327 241 193 155 89 30
BanII G_rGCy'C
Cuts at: 0 445 453 535 541 577 763 866 1035
Size: 445 8 82 6 36 186 103 169
Fragments arranged by size:
445 186 169 103 82 36 8 6
BbeI G_GCGC'C
Cuts at: 0 420 613 1035
Size: 420 193 422
Fragments arranged by size:
422 420 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 515 549 1035
Size: 515 34 486
Fragments arranged by size:
515 486 34
BglI GCCn_nnn'nGGC
Cuts at: 0 103 457 1035
Size: 103 354 578
Fragments arranged by size:
578 354 103
Bme1580I G_kGCm'C
Cuts at: 0 332 445 453 541 577 866 1035
Size: 332 113 8 88 36 289 169
Fragments arranged by size:
332 289 169 113 88 36 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 1035
Size: 888 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 127 1035
Size: 66 61 908
Fragments arranged by size:
908 66 61
BsaHI Gr'CG_yC
Cuts at: 0 417 610 1035
Size: 417 193 425
Fragments arranged by size:
425 417 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 65 95 1035
Size: 65 30 940
Fragments arranged by size:
940 65 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 891 1035
Size: 891 144
BsiEI CG_ry'CG
Cuts at: 0 349 680 689 873 1035
Size: 349 331 9 184 162
Fragments arranged by size:
349 331 184 162 9
BsiHKAI G_wGCw'C
Cuts at: 0 763 1035
Size: 763 272
BsmBI CGTCTCn'nnnn_
Cuts at: 0 472 1035
Size: 472 563
Bsp1286I G_dGCh'C
Cuts at: 0 332 445 453 535 541 577 763 866
Size: 332 113 8 82 6 36 186 103
Cuts at: 866 1035
Size: 169
Fragments arranged by size:
332 186 169 113 103 82 36 8
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 996 1035
Size: 996 39
BsrBI CCG'CTC
Cuts at: 0 521 1035
Size: 521 514
BsrFI r'CCGG_y
Cuts at: 0 266 413 606 1035
Size: 266 147 193 429
Fragments arranged by size:
429 266 193 147
BssHII G'CGCG_C
Cuts at: 0 700 1035
Size: 700 335
BstYI r'GATC_y
Cuts at: 0 977 1035
Size: 977 58
BtgI C'CryG_G
Cuts at: 0 101 176 569 680 1035
Size: 101 75 393 111 355
Fragments arranged by size:
393 355 111 101 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 147 156 1035
Size: 147 9 879
Fragments arranged by size:
879 147 9
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 949 1035
Size: 319 630 86
Fragments arranged by size:
630 319 86
EaeI y'GGCC_r
Cuts at: 0 35 346 622 677 690 870 893 1035
Size: 35 311 276 55 13 180 23 142
Fragments arranged by size:
311 276 180 142 55 35 23 13
EagI C'GGCC_G
Cuts at: 0 346 677 870 1035
Size: 346 331 193 165
Fragments arranged by size:
346 331 193 165
EarI CTCTTCn'nnn_
Cuts at: 0 16 418 751 1035
Size: 16 402 333 284
Fragments arranged by size:
402 333 284 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 280 593 905 1035
Size: 280 313 312 130
Fragments arranged by size:
313 312 280 130
EcoICRI GAG'CTC
Cuts at: 0 761 1035
Size: 761 274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 812 888 1035
Size: 765 47 76 147
Fragments arranged by size:
765 147 76 47
EcoO109I rG'GnC_Cy
Cuts at: 0 442 449 450 467 538 574 722 1035
Size: 442 7 1 17 71 36 148 313
Fragments arranged by size:
442 313 148 71 36 17 7 1
HaeII r_GCGC'y
Cuts at: 0 420 568 613 1035
Size: 420 148 45 422
Fragments arranged by size:
422 420 148 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 583 615 1035
Size: 65 32 486 32 420
Fragments arranged by size:
486 420 65 32 32
KasI G'GCGC_C
Cuts at: 0 416 609 1035
Size: 416 193 426
Fragments arranged by size:
426 416 193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1035
Size: 788 247
MslI CAynn'nnrTG
Cuts at: 0 175 1035
Size: 175 860
MspA1I CmG'CkG
Cuts at: 0 103 383 571 676 1035
Size: 103 280 188 105 359
Fragments arranged by size:
359 280 188 105 103
NaeI GCC'GGC
Cuts at: 0 608 1035
Size: 608 427
NarI GG'CG_CC
Cuts at: 0 417 610 1035
Size: 417 193 425
Fragments arranged by size:
425 417 193
NcoI C'CATG_G
Cuts at: 0 176 1035
Size: 176 859
NgoMIV G'CCGG_C
Cuts at: 0 606 1035
Size: 606 429
NspI r_CATG'y
Cuts at: 0 396 952 1035
Size: 396 556 83
Fragments arranged by size:
556 396 83
PasI CC'CwG_GG
Cuts at: 0 666 1035
Size: 666 369
PciI A'CATG_T
Cuts at: 0 392 1035
Size: 392 643
PfoI T'CCnGG_A
Cuts at: 0 185 587 1035
Size: 185 402 448
Fragments arranged by size:
448 402 185
PpuMI rG'GwC_Cy
Cuts at: 0 722 1035
Size: 722 313
PspOMI G'GGCC_C
Cuts at: 0 441 449 537 573 862 1035
Size: 441 8 88 36 289 173
Fragments arranged by size:
441 289 173 88 36 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 394 426 1035
Size: 394 32 609
Fragments arranged by size:
609 394 32
PstI C_TGCA'G
Cuts at: 0 560 1007 1035
Size: 560 447 28
Fragments arranged by size:
560 447 28
SacI G_AGCT'C
Cuts at: 0 763 1035
Size: 763 272
SacII CC_GC'GG
Cuts at: 0 104 572 1035
Size: 104 468 463
Fragments arranged by size:
468 463 104
SanDI GG'GwC_CC
Cuts at: 0 722 1035
Size: 722 313
SapI GCTCTTCn'nnn_
Cuts at: 0 751 1035
Size: 751 284
SbfI CC_TGCA'GG
Cuts at: 0 560 1007 1035
Size: 560 447 28
Fragments arranged by size:
560 447 28
SfcI C'TryA_G
Cuts at: 0 153 556 1003 1035
Size: 153 403 447 32
Fragments arranged by size:
447 403 153 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 457 1035
Size: 457 578
SfoI GGC'GCC
Cuts at: 0 418 611 1035
Size: 418 193 424
Fragments arranged by size:
424 418 193
SgrAI Cr'CCGG_yG
Cuts at: 0 606 1035
Size: 606 429
SmaI CCC'GGG
Cuts at: 0 447 719 1035
Size: 447 272 316
Fragments arranged by size:
447 316 272
StyI C'CwwG_G
Cuts at: 0 176 843 1035
Size: 176 667 192
Fragments arranged by size:
667 192 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 1035
Size: 868 167
Tth111I GACn'n_nGTC
Cuts at: 0 633 1035
Size: 633 402
XmaI C'CCGG_G
Cuts at: 0 445 717 1035
Size: 445 272 318
Fragments arranged by size:
445 318 272
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI AvrII BamHI BanI
BanII BbeI BcgI BglI Bme1580I BpmI BsaI BsaHI
BsaXI BseRI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstYI BtgI BtgZI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PpuMI PspOMI PsrI PstI SacI
SacII SanDI SapI SbfI SfcI SfiI SfoI SgrAI
SmaI StyI TaqII Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI BaeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BseYI BsgI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NdeI NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI PvuII RsrII SalI ScaI SexAI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI XbaI XcmI XhoI XmnI ZraI