Restriction map for
pF1KB9607
(Linear) (Six-base) MAPSORT of: KIBB9607.seq Check: 4154 from: 1 to: 591
KIBB9607.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 591
Size: 289 302
AflIII A'CryG_T
Cuts at: 0 293 591
Size: 293 298
AhdI GACnn_n'nnGTC
Cuts at: 0 207 591
Size: 207 384
AlwNI CAG_nnn'CTG
Cuts at: 0 33 97 591
Size: 33 64 494
Fragments arranged by size:
494 64 33
ApaI G_GGCC'C
Cuts at: 0 67 591
Size: 67 524
ApaLI G'TGCA_C
Cuts at: 0 496 591
Size: 496 95
BanI G'GyrC_C
Cuts at: 0 395 591
Size: 395 196
BanII G_rGCy'C
Cuts at: 0 67 240 591
Size: 67 173 351
Fragments arranged by size:
351 173 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 276 591
Size: 276 315
BbvCI CC'TCA_GC
Cuts at: 0 26 591
Size: 26 565
BglI GCCn_nnn'nGGC
Cuts at: 0 235 591
Size: 235 356
Bme1580I G_kGCm'C
Cuts at: 0 67 500 591
Size: 67 433 91
Fragments arranged by size:
433 91 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 591
Size: 224 367
Bpu10I CC'TnA_GC
Cuts at: 0 26 591
Size: 26 565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 591
Size: 261 330
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 591
Size: 46 545
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 128 220 591
Size: 99 29 92 371
Fragments arranged by size:
371 99 92 29
BseYI C'CCAG_C
Cuts at: 0 482 591
Size: 482 109
BsiHKAI G_wGCw'C
Cuts at: 0 277 500 591
Size: 277 223 91
Fragments arranged by size:
277 223 91
BsmI GAATG_Cn'
Cuts at: 0 367 591
Size: 367 224
Bsp1286I G_dGCh'C
Cuts at: 0 67 240 277 500 591
Size: 67 173 37 223 91
Fragments arranged by size:
223 173 91 67 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 591
Size: 167 424
BsrBI CCG'CTC
Cuts at: 0 86 444 591
Size: 86 358 147
Fragments arranged by size:
358 147 86
BssHII G'CGCG_C
Cuts at: 0 375 591
Size: 375 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 387 411 591
Size: 387 24 180
Fragments arranged by size:
387 180 24
BstYI r'GATC_y
Cuts at: 0 118 591
Size: 118 473
BtgI C'CryG_G
Cuts at: 0 56 432 591
Size: 56 376 159
Fragments arranged by size:
376 159 56
BtsI GCAGTG_nn'
Cuts at: 0 528 559 591
Size: 528 31 32
Fragments arranged by size:
528 32 31
DraIII CAC_nnn'GTG
Cuts at: 0 578 591
Size: 578 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 289 591
Size: 224 65 302
Fragments arranged by size:
302 224 65
EcoO109I rG'GnC_Cy
Cuts at: 0 63 64 591
Size: 63 1 527
Fragments arranged by size:
527 63 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 280 302 312 334 591
Size: 193 32 55 22 10 22 257
Fragments arranged by size:
257 193 55 32 22 22 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 591
Size: 55 536
MspA1I CmG'CkG
Cuts at: 0 263 591
Size: 263 328
NcoI C'CATG_G
Cuts at: 0 56 591
Size: 56 535
NspI r_CATG'y
Cuts at: 0 297 591
Size: 297 294
PciI A'CATG_T
Cuts at: 0 293 591
Size: 293 298
PflMI CCAn_nnn'nTGG
Cuts at: 0 97 591
Size: 97 494
PfoI T'CCnGG_A
Cuts at: 0 461 591
Size: 461 130
PspOMI G'GGCC_C
Cuts at: 0 63 591
Size: 63 528
PstI C_TGCA'G
Cuts at: 0 566 591
Size: 566 25
PvuII CAG'CTG
Cuts at: 0 263 591
Size: 263 328
SfcI C'TryA_G
Cuts at: 0 258 562 591
Size: 258 304 29
Fragments arranged by size:
304 258 29
SmlI C'TyrA_G
Cuts at: 0 276 591
Size: 276 315
StyI C'CwwG_G
Cuts at: 0 56 142 591
Size: 56 86 449
Fragments arranged by size:
449 86 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 591
Size: 337 254
TatI w'GTAC_w
Cuts at: 0 290 591
Size: 290 301
XmnI GAAnn'nnTTC
Cuts at: 0 367 591
Size: 367 224
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApaI ApaLI BanI BanII
BbsI BbvCI BglI Bme1580I BpmI Bpu10I BpuEI BsaI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BssHII
BstXI BstYI BtgI BtsI DraIII Eco57MI EcoO109I Hin4I
MmeI MspA1I NcoI NspI PciI PflMI PfoI PspOMI
PstI PvuII SfcI SmlI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI