Restriction map for
pF1KB9611
(Linear) (Six-base) MAPSORT of: KIBB9611.seq Check: 2997 from: 1 to: 1767
KIBB9611.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1766 1767
Size: 1766 1
AatII G_ACGT'C
Cuts at: 0 143 1767
Size: 143 1624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 1667 1767
Size: 1351 316 100
Fragments arranged by size:
1351 316 100
AhdI GACnn_n'nnGTC
Cuts at: 0 1374 1767
Size: 1374 393
AleI CACnn'nnGTG
Cuts at: 0 1708 1767
Size: 1708 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 475 1767
Size: 475 1292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1767
Size: 1045 32 690
Fragments arranged by size:
1045 690 32
AlwNI CAG_nnn'CTG
Cuts at: 0 857 1459 1748 1767
Size: 857 602 289 19
Fragments arranged by size:
857 602 289 19
ApaLI G'TGCA_C
Cuts at: 0 520 1767
Size: 520 1247
ApoI r'AATT_y
Cuts at: 0 1504 1767
Size: 1504 263
AvaI C'yCGr_G
Cuts at: 0 591 688 1767
Size: 591 97 1079
Fragments arranged by size:
1079 591 97
BamHI G'GATC_C
Cuts at: 0 824 1767
Size: 824 943
BanI G'GyrC_C
Cuts at: 0 1113 1767
Size: 1113 654
BanII G_rGCy'C
Cuts at: 0 695 1048 1767
Size: 695 353 719
Fragments arranged by size:
719 695 353
BbeI G_GCGC'C
Cuts at: 0 1117 1767
Size: 1117 650
BbsI GAAGACnn'nnnn_
Cuts at: 0 621 1197 1767
Size: 621 576 570
Fragments arranged by size:
621 576 570
BbvCI CC'TCA_GC
Cuts at: 0 1005 1767
Size: 1005 762
BglI GCCn_nnn'nGGC
Cuts at: 0 190 1519 1767
Size: 190 1329 248
Fragments arranged by size:
1329 248 190
BglII A'GATC_T
Cuts at: 0 926 1478 1767
Size: 926 552 289
Fragments arranged by size:
926 552 289
BlpI GC'TnA_GC
Cuts at: 0 1146 1151 1767
Size: 1146 5 616
Fragments arranged by size:
1146 616 5
Bme1580I G_kGCm'C
Cuts at: 0 524 864 1435 1715 1767
Size: 524 340 571 280 52
Fragments arranged by size:
571 524 340 280 52
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 765 1767
Size: 765 1002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 439 625 628 685 735 1177 1767
Size: 313 126 186 3 57 50 442 590
Fragments arranged by size:
590 442 313 186 126 57 50 3
Bpu10I CC'TnA_GC
Cuts at: 0 1005 1767
Size: 1005 762
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 601 1065 1534 1767
Size: 13 588 464 469 233
Fragments arranged by size:
588 469 464 233 13
BsaBI GATnn'nnATC
Cuts at: 0 1483 1767
Size: 1483 284
BsaHI Gr'CG_yC
Cuts at: 0 140 470 1114 1298 1767
Size: 140 330 644 184 469
Fragments arranged by size:
644 469 330 184 140
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 1045 1075 1396 1426 1767
Size: 61 30 954 30 321 30 341
Fragments arranged by size:
954 341 321 61 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 309 355 685 754 1767
Size: 309 46 330 69 1013
Fragments arranged by size:
1013 330 309 69 46
BseYI C'CCAG_C
Cuts at: 0 979 1436 1767
Size: 979 457 331
Fragments arranged by size:
979 457 331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1767
Size: 262 1505
BsiEI CG_ry'CG
Cuts at: 0 162 1013 1767
Size: 162 851 754
Fragments arranged by size:
851 754 162
BsiHKAI G_wGCw'C
Cuts at: 0 524 695 1767
Size: 524 171 1072
Fragments arranged by size:
1072 524 171
BsmBI CGTCTCn'nnnn_
Cuts at: 0 523 753 1767
Size: 523 230 1014
Fragments arranged by size:
1014 523 230
Bsp1286I G_dGCh'C
Cuts at: 0 524 695 864 1048 1435 1715 1767
Size: 524 171 169 184 387 280 52
Fragments arranged by size:
524 387 280 184 171 169 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 284 983 1766 1767
Size: 284 699 783 1
Fragments arranged by size:
783 699 284 1
BsrBI CCG'CTC
Cuts at: 0 621 1767
Size: 621 1146
BsrDI GCAATG_nn'
Cuts at: 0 1311 1767
Size: 1311 456
BsrFI r'CCGG_y
Cuts at: 0 485 1767
Size: 485 1282
BstAPI GCAn_nnn'nTGC
Cuts at: 0 125 1589 1767
Size: 125 1464 178
Fragments arranged by size:
1464 178 125
BstEII G'GTnAC_C
Cuts at: 0 846 1767
Size: 846 921
BstXI CCAn_nnnn'nTGG
Cuts at: 0 447 1767
Size: 447 1320
BstYI r'GATC_y
Cuts at: 0 824 926 1478 1767
Size: 824 102 552 289
Fragments arranged by size:
824 552 289 102
BtgI C'CryG_G
Cuts at: 0 228 670 1140 1730 1767
Size: 228 442 470 590 37
Fragments arranged by size:
590 470 442 228 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 312 1767
Size: 312 1455
BtsI GCAGTG_nn'
Cuts at: 0 123 574 1319 1767
Size: 123 451 745 448
Fragments arranged by size:
745 451 448 123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1719 1754 1767
Size: 1719 35 13
Fragments arranged by size:
1719 35 13
DraIII CAC_nnn'GTG
Cuts at: 0 393 1767
Size: 393 1374
DrdI GACnn_nn'nnGTC
Cuts at: 0 27 1767
Size: 27 1740
EaeI y'GGCC_r
Cuts at: 0 191 865 1137 1767
Size: 191 674 272 630
Fragments arranged by size:
674 630 272 191
EarI CTCTTCn'nnn_
Cuts at: 0 70 1767
Size: 70 1697
EcoICRI GAG'CTC
Cuts at: 0 693 1767
Size: 693 1074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 439 625 628 685 735 1177 1351
Size: 313 126 186 3 57 50 442 174
Cuts at: 1351 1667 1767
Size: 316 100
Fragments arranged by size:
442 316 313 186 174 126 100 57
50 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 565 739 1767
Size: 565 174 1028
Fragments arranged by size:
1028 565 174
EcoO109I rG'GnC_Cy
Cuts at: 0 759 804 1643 1767
Size: 759 45 839 124
Fragments arranged by size:
839 759 124 45
EcoRI G'AATT_C
Cuts at: 0 1504 1767
Size: 1504 263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 699 1767
Size: 699 1068
HaeII r_GCGC'y
Cuts at: 0 1004 1117 1767
Size: 1004 113 650
Fragments arranged by size:
1004 650 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 574 606 1087 1119 1360 1392 1767
Size: 574 32 481 32 241 32 375
Fragments arranged by size:
574 481 375 241 32 32 32
HincII GTy'rAC
Cuts at: 0 261 1767
Size: 261 1506
HindIII A'AGCT_T
Cuts at: 0 787 1767
Size: 787 980
KasI G'GCGC_C
Cuts at: 0 1113 1767
Size: 1113 654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 464 752 1767
Size: 341 123 288 1015
Fragments arranged by size:
1015 341 288 123
MscI TGG'CCA
Cuts at: 0 1139 1767
Size: 1139 628
MslI CAynn'nnrTG
Cuts at: 0 1708 1767
Size: 1708 59
MspA1I CmG'CkG
Cuts at: 0 1150 1459 1767
Size: 1150 309 308
Fragments arranged by size:
1150 309 308
NaeI GCC'GGC
Cuts at: 0 487 1767
Size: 487 1280
NarI GG'CG_CC
Cuts at: 0 1114 1767
Size: 1114 653
NcoI C'CATG_G
Cuts at: 0 228 1140 1767
Size: 228 912 627
Fragments arranged by size:
912 627 228
NgoMIV G'CCGG_C
Cuts at: 0 485 1767
Size: 485 1282
NspI r_CATG'y
Cuts at: 0 132 378 513 1587 1767
Size: 132 246 135 1074 180
Fragments arranged by size:
1074 246 180 135 132
PasI CC'CwG_GG
Cuts at: 0 1255 1767
Size: 1255 512
PflMI CCAn_nnn'nTGG
Cuts at: 0 1268 1550 1599 1767
Size: 1268 282 49 168
Fragments arranged by size:
1268 282 168 49
PfoI T'CCnGG_A
Cuts at: 0 266 416 1767
Size: 266 150 1351
Fragments arranged by size:
1351 266 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 1045 1077 1767
Size: 205 32 808 32 690
Fragments arranged by size:
808 690 205 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1643 1767
Size: 1643 124
PstI C_TGCA'G
Cuts at: 0 383 740 833 1767
Size: 383 357 93 934
Fragments arranged by size:
934 383 357 93
PvuI CG_AT'CG
Cuts at: 0 1013 1767
Size: 1013 754
PvuII CAG'CTG
Cuts at: 0 1150 1767
Size: 1150 617
SacI G_AGCT'C
Cuts at: 0 695 1767
Size: 695 1072
SanDI GG'GwC_CC
Cuts at: 0 1643 1767
Size: 1643 124
SbfI CC_TGCA'GG
Cuts at: 0 740 1767
Size: 740 1027
SexAI A'CCwGG_T
Cuts at: 0 932 1767
Size: 932 835
SfcI C'TryA_G
Cuts at: 0 379 736 829 1767
Size: 379 357 93 938
Fragments arranged by size:
938 379 357 93
SfoI GGC'GCC
Cuts at: 0 1115 1767
Size: 1115 652
SmlI C'TyrA_G
Cuts at: 0 591 1767
Size: 591 1176
StuI AGG'CCT
Cuts at: 0 76 1767
Size: 76 1691
StyI C'CwwG_G
Cuts at: 0 228 566 950 965 1064 1106 1140 1379
Size: 228 338 384 15 99 42 34 239
Cuts at: 1379 1441 1767
Size: 62 326
Fragments arranged by size:
384 338 326 239 228 99 62 42
34 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 625 639 1065 1767
Size: 625 14 426 702
Fragments arranged by size:
702 625 426 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 662 694 1396 1428 1767
Size: 61 32 569 32 702 32 339
Fragments arranged by size:
702 569 339 61 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 256 1767
Size: 256 1511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 1767
Size: 792 975
XhoI C'TCGA_G
Cuts at: 0 591 1767
Size: 591 1176
XmnI GAAnn'nnTTC
Cuts at: 0 430 994 1767
Size: 430 564 773
Fragments arranged by size:
773 564 430
ZraI GAC'GTC
Cuts at: 0 141 1767
Size: 141 1626
Enzymes that do cut:
AarI AatII AcuI AhdI AleI AlfI AloI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PstI PvuI PvuII SacI
SanDI SbfI SexAI SfcI SfoI SmlI StuI StyI
TaqII TstI Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI ApaI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BfrBI BmgBI BmrI BmtI BpuEI BsaAI BsaWI BsiWI
BsmI BspEI BspHI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I ClaI DraI EagI EciI EcoRV FseI FspI
FspAI HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI RsrII SacII SalI SapI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI XbaI XmaI