Restriction map for
pF1KB9619
(Linear) (Six-base) MAPSORT of: KIBB9619.seq Check: 5991 from: 1 to: 1041
KIBB9619.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 948 1041
Size: 948 93
AccI GT'mk_AC
Cuts at: 0 515 1041
Size: 515 526
AflIII A'CryG_T
Cuts at: 0 467 1041
Size: 467 574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 841 1041
Size: 841 200
AlwNI CAG_nnn'CTG
Cuts at: 0 574 1014 1041
Size: 574 440 27
Fragments arranged by size:
574 440 27
ApaI G_GGCC'C
Cuts at: 0 69 494 506 1041
Size: 69 425 12 535
Fragments arranged by size:
535 425 69 12
ApaLI G'TGCA_C
Cuts at: 0 3 1041
Size: 3 1038
AvaI C'yCGr_G
Cuts at: 0 315 505 582 1041
Size: 315 190 77 459
Fragments arranged by size:
459 315 190 77
BanI G'GyrC_C
Cuts at: 0 589 703 1041
Size: 589 114 338
Fragments arranged by size:
589 338 114
BanII G_rGCy'C
Cuts at: 0 69 165 220 494 506 619 1041
Size: 69 96 55 274 12 113 422
Fragments arranged by size:
422 274 113 96 69 55 12
BbeI G_GCGC'C
Cuts at: 0 593 707 1041
Size: 593 114 334
Fragments arranged by size:
593 334 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 797 907 1041
Size: 797 110 134
Fragments arranged by size:
797 134 110
BbvCI CC'TCA_GC
Cuts at: 0 553 1041
Size: 553 488
BciVI GTATCCnnnnn_n'
Cuts at: 0 49 1041
Size: 49 992
BclI T'GATC_A
Cuts at: 0 293 1041
Size: 293 748
BglI GCCn_nnn'nGGC
Cuts at: 0 182 1041
Size: 182 859
Bme1580I G_kGCm'C
Cuts at: 0 7 69 494 506 1041
Size: 7 62 425 12 535
Fragments arranged by size:
535 425 62 12 7
BmgBI CAC'GTC
Cuts at: 0 993 1041
Size: 993 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 907 1041
Size: 889 18 134
Fragments arranged by size:
889 134 18
Bpu10I CC'TnA_GC
Cuts at: 0 553 1041
Size: 553 488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 892 1041
Size: 223 669 149
Fragments arranged by size:
669 223 149
BsaAI yAC'GTr
Cuts at: 0 453 1041
Size: 453 588
BsaHI Gr'CG_yC
Cuts at: 0 304 380 512 590 704 758 1041
Size: 304 76 132 78 114 54 283
Fragments arranged by size:
304 283 132 114 78 76 54
BsaWI w'CCGG_w
Cuts at: 0 140 1041
Size: 140 901
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 937 1041
Size: 403 534 104
Fragments arranged by size:
534 403 104
BseYI C'CCAG_C
Cuts at: 0 499 567 687 965 1041
Size: 499 68 120 278 76
Fragments arranged by size:
499 278 120 76 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 421 1041
Size: 380 41 620
Fragments arranged by size:
620 380 41
BsiEI CG_ry'CG
Cuts at: 0 63 1041
Size: 63 978
BsiHKAI G_wGCw'C
Cuts at: 0 7 619 1041
Size: 7 612 422
Fragments arranged by size:
612 422 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 239 1041
Size: 239 802
Bsp1286I G_dGCh'C
Cuts at: 0 7 69 165 220 494 506 619 1041
Size: 7 62 96 55 274 12 113 422
Fragments arranged by size:
422 274 113 96 62 55 12 7
BspHI T'CATG_A
Cuts at: 0 983 1041
Size: 983 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 948 1041
Size: 948 93
BsrFI r'CCGG_y
Cuts at: 0 226 520 667 768 833 940 1041
Size: 226 294 147 101 65 107 101
Fragments arranged by size:
294 226 147 107 101 101 65
BssHII G'CGCG_C
Cuts at: 0 745 880 1041
Size: 745 135 161
Fragments arranged by size:
745 161 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 735 1041
Size: 735 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 786 1041
Size: 786 255
BtgI C'CryG_G
Cuts at: 0 336 699 1041
Size: 336 363 342
Fragments arranged by size:
363 342 336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 861 1041
Size: 861 180
DraIII CAC_nnn'GTG
Cuts at: 0 851 1041
Size: 851 190
DrdI GACnn_nn'nnGTC
Cuts at: 0 415 1041
Size: 415 626
EaeI y'GGCC_r
Cuts at: 0 60 128 672 842 1041
Size: 60 68 544 170 199
Fragments arranged by size:
544 199 170 68 60
EagI C'GGCC_G
Cuts at: 0 60 1041
Size: 60 981
EarI CTCTTCn'nnn_
Cuts at: 0 255 1041
Size: 255 786
EcoICRI GAG'CTC
Cuts at: 0 617 1041
Size: 617 424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 907 1041
Size: 889 18 134
Fragments arranged by size:
889 134 18
EcoO109I rG'GnC_Cy
Cuts at: 0 66 490 491 1041
Size: 66 424 1 550
Fragments arranged by size:
550 424 66 1
HaeII r_GCGC'y
Cuts at: 0 157 482 593 707 1041
Size: 157 325 111 114 334
Fragments arranged by size:
334 325 157 114 111
HincII GTy'rAC
Cuts at: 0 123 1041
Size: 123 918
KasI G'GCGC_C
Cuts at: 0 589 703 1041
Size: 589 114 338
Fragments arranged by size:
589 338 114
MscI TGG'CCA
Cuts at: 0 674 1041
Size: 674 367
MslI CAynn'nnrTG
Cuts at: 0 10 784 808 1041
Size: 10 774 24 233
Fragments arranged by size:
774 233 24 10
MspA1I CmG'CkG
Cuts at: 0 338 350 687 701 1011 1041
Size: 338 12 337 14 310 30
Fragments arranged by size:
338 337 310 30 14 12
NaeI GCC'GGC
Cuts at: 0 522 942 1041
Size: 522 420 99
Fragments arranged by size:
522 420 99
NarI GG'CG_CC
Cuts at: 0 590 704 1041
Size: 590 114 337
Fragments arranged by size:
590 337 114
NgoMIV G'CCGG_C
Cuts at: 0 520 940 1041
Size: 520 420 101
Fragments arranged by size:
520 420 101
PasI CC'CwG_GG
Cuts at: 0 474 1041
Size: 474 567
PfoI T'CCnGG_A
Cuts at: 0 950 1041
Size: 950 91
PmlI CAC'GTG
Cuts at: 0 453 1041
Size: 453 588
PshAI GACnn'nnGTC
Cuts at: 0 991 1041
Size: 991 50
PspOMI G'GGCC_C
Cuts at: 0 65 490 502 1041
Size: 65 425 12 539
Fragments arranged by size:
539 425 65 12
PvuII CAG'CTG
Cuts at: 0 687 1011 1041
Size: 687 324 30
Fragments arranged by size:
687 324 30
SacI G_AGCT'C
Cuts at: 0 619 1041
Size: 619 422
SacII CC_GC'GG
Cuts at: 0 339 702 1041
Size: 339 363 339
Fragments arranged by size:
363 339 339
ScaI AGT'ACT
Cuts at: 0 325 1041
Size: 325 716
SfoI GGC'GCC
Cuts at: 0 591 705 1041
Size: 591 114 336
Fragments arranged by size:
591 336 114
SgrAI Cr'CCGG_yG
Cuts at: 0 226 667 768 1041
Size: 226 441 101 273
Fragments arranged by size:
441 273 226 101
SmaI CCC'GGG
Cuts at: 0 317 507 1041
Size: 317 190 534
Fragments arranged by size:
534 317 190
SmlI C'TyrA_G
Cuts at: 0 238 907 1041
Size: 238 669 134
Fragments arranged by size:
669 238 134
TatI w'GTAC_w
Cuts at: 0 323 1041
Size: 323 718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 1041
Size: 471 570
XmaI C'CCGG_G
Cuts at: 0 315 505 1041
Size: 315 190 536
Fragments arranged by size:
536 315 190
Enzymes that do cut:
AarI AccI AflIII AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrFI BssHII BstAPI BstXI BtgI BtgZI DraIII
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII
HincII KasI MscI MslI MspA1I NaeI NarI NgoMIV
PasI PfoI PmlI PshAI PspOMI PvuII SacI SacII
ScaI SfoI SgrAI SmaI SmlI TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BfrBI BglII BlpI BmrI BmtI BplI
BsaI BsaBI BsaXI BsiWI BsmI BspEI BsrBI BsrDI
BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PpiI PpuMI PsiI
PspXI PsrI PstI PvuI RsrII SalI SanDI SapI
SbfI SexAI SfcI SfiI SgfI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XhoI XmnI ZraI