Restriction map for
pF1KB9629
(Linear) (Six-base) MAPSORT of: KIBB9629.seq Check: 1623 from: 1 to: 1011
KIBB9629.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1011
Size: 838 173
AflII C'TTAA_G
Cuts at: 0 348 777 1011
Size: 348 429 234
Fragments arranged by size:
429 348 234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 890 1011
Size: 890 121
AlwNI CAG_nnn'CTG
Cuts at: 0 387 1011
Size: 387 624
ApaI G_GGCC'C
Cuts at: 0 153 1011
Size: 153 858
ApoI r'AATT_y
Cuts at: 0 443 1011
Size: 443 568
AvaI C'yCGr_G
Cuts at: 0 657 1011
Size: 657 354
BanI G'GyrC_C
Cuts at: 0 907 1011
Size: 907 104
BanII G_rGCy'C
Cuts at: 0 153 647 1011
Size: 153 494 364
Fragments arranged by size:
494 364 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 194 1011
Size: 194 817
BfrBI ATG'CAT
Cuts at: 0 867 1011
Size: 867 144
BglII A'GATC_T
Cuts at: 0 468 1011
Size: 468 543
Bme1580I G_kGCm'C
Cuts at: 0 153 435 1011
Size: 153 282 576
Fragments arranged by size:
576 282 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1011
Size: 176 835
Bpu10I CC'TnA_GC
Cuts at: 0 641 1011
Size: 641 370
BsaI GGTCTCn'nnnn_
Cuts at: 0 456 1011
Size: 456 555
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 1011
Size: 196 815
BseYI C'CCAG_C
Cuts at: 0 509 1011
Size: 509 502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 572 896 1011
Size: 72 500 324 115
Fragments arranged by size:
500 324 115 72
BsiHKAI G_wGCw'C
Cuts at: 0 647 1011
Size: 647 364
BsmI GAATG_Cn'
Cuts at: 0 76 1011
Size: 76 935
Bsp1286I G_dGCh'C
Cuts at: 0 153 435 647 1011
Size: 153 282 212 364
Fragments arranged by size:
364 282 212 153
BssHII G'CGCG_C
Cuts at: 0 301 1011
Size: 301 710
BstEII G'GTnAC_C
Cuts at: 0 602 1011
Size: 602 409
BstYI r'GATC_y
Cuts at: 0 468 1011
Size: 468 543
BtsI GCAGTG_nn'
Cuts at: 0 717 884 1011
Size: 717 167 127
Fragments arranged by size:
717 167 127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 509 544 1011
Size: 509 35 467
Fragments arranged by size:
509 467 35
EaeI y'GGCC_r
Cuts at: 0 413 1011
Size: 413 598
EarI CTCTTCn'nnn_
Cuts at: 0 173 191 1011
Size: 173 18 820
Fragments arranged by size:
820 173 18
EcoICRI GAG'CTC
Cuts at: 0 645 1011
Size: 645 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 838 1011
Size: 176 662 173
Fragments arranged by size:
662 176 173
EcoNI CCTnn'n_nnAGG
Cuts at: 0 901 1011
Size: 901 110
EcoO109I rG'GnC_Cy
Cuts at: 0 149 150 772 892 1011
Size: 149 1 622 120 119
Fragments arranged by size:
622 149 120 119 1
FspI TGC'GCA
Cuts at: 0 961 1011
Size: 961 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 453 485 1011
Size: 453 32 526
Fragments arranged by size:
526 453 32
HincII GTy'rAC
Cuts at: 0 5 1011
Size: 5 1006
HindIII A'AGCT_T
Cuts at: 0 105 1011
Size: 105 906
HpaI GTT'AAC
Cuts at: 0 5 1011
Size: 5 1006
MscI TGG'CCA
Cuts at: 0 415 1011
Size: 415 596
MslI CAynn'nnrTG
Cuts at: 0 872 1011
Size: 872 139
NsiI A_TGCA'T
Cuts at: 0 869 1011
Size: 869 142
NspI r_CATG'y
Cuts at: 0 867 1011
Size: 867 144
PasI CC'CwG_GG
Cuts at: 0 887 1011
Size: 887 124
PpuMI rG'GwC_Cy
Cuts at: 0 772 892 1011
Size: 772 120 119
Fragments arranged by size:
772 120 119
PspOMI G'GGCC_C
Cuts at: 0 149 1011
Size: 149 862
SacI G_AGCT'C
Cuts at: 0 647 1011
Size: 647 364
SanDI GG'GwC_CC
Cuts at: 0 892 1011
Size: 892 119
ScaI AGT'ACT
Cuts at: 0 738 1011
Size: 738 273
SmaI CCC'GGG
Cuts at: 0 659 1011
Size: 659 352
SmlI C'TyrA_G
Cuts at: 0 348 777 1011
Size: 348 429 234
Fragments arranged by size:
429 348 234
SphI G_CATG'C
Cuts at: 0 867 1011
Size: 867 144
StyI C'CwwG_G
Cuts at: 0 833 897 1011
Size: 833 64 114
Fragments arranged by size:
833 114 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 900 1011
Size: 900 111
TatI w'GTAC_w
Cuts at: 0 736 1011
Size: 736 275
XbaI T'CTAG_A
Cuts at: 0 64 1011
Size: 64 947
XmaI C'CCGG_G
Cuts at: 0 657 1011
Size: 657 354
XmnI GAAnn'nnTTC
Cuts at: 0 60 1011
Size: 60 951
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BfrBI BglII Bme1580I BpmI Bpu10I BsaI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BssHII BstEII
BstYI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI Hin4I HincII HindIII HpaI MscI MslI
NsiI NspI PasI PpuMI PspOMI SacI SanDI ScaI
SmaI SmlI SphI StyI TaqII TatI XbaI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI
BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI
HaeII KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XcmI XhoI ZraI