Restriction map for
pF1KB9631
(Linear) (Six-base) MAPSORT of: KIBB9631.seq Check: 6350 from: 1 to: 816
KIBB9631.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AccI GT'mk_AC
Cuts at: 0 339 816
Size: 339 477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 816
Size: 364 452
AflIII A'CryG_T
Cuts at: 0 44 816
Size: 44 772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 797 816
Size: 797 19
AvaI C'yCGr_G
Cuts at: 0 272 290 427 579 816
Size: 272 18 137 152 237
Fragments arranged by size:
272 237 152 137 18
BanI G'GyrC_C
Cuts at: 0 263 295 586 816
Size: 263 32 291 230
Fragments arranged by size:
291 263 230 32
BanII G_rGCy'C
Cuts at: 0 580 816
Size: 580 236
BbeI G_GCGC'C
Cuts at: 0 267 299 590 816
Size: 267 32 291 226
Fragments arranged by size:
291 267 226 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 337 816
Size: 337 479
BbvCI CC'TCA_GC
Cuts at: 0 170 816
Size: 170 646
BglI GCCn_nnn'nGGC
Cuts at: 0 82 231 304 584 727 816
Size: 82 149 73 280 143 89
Fragments arranged by size:
280 149 143 89 82 73
BlpI GC'TnA_GC
Cuts at: 0 574 816
Size: 574 242
Bme1580I G_kGCm'C
Cuts at: 0 489 816
Size: 489 327
BmgBI CAC'GTC
Cuts at: 0 45 816
Size: 45 771
Bpu10I CC'TnA_GC
Cuts at: 0 170 601 816
Size: 170 431 215
Fragments arranged by size:
431 215 170
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 448 490 816
Size: 10 438 42 326
Fragments arranged by size:
438 326 42 10
BsaAI yAC'GTr
Cuts at: 0 638 816
Size: 638 178
BsaHI Gr'CG_yC
Cuts at: 0 264 296 434 587 816
Size: 264 32 138 153 229
Fragments arranged by size:
264 229 153 138 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 776 806 816
Size: 776 30 10
Fragments arranged by size:
776 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 139 142 660 816
Size: 136 3 3 518 156
Fragments arranged by size:
518 156 136 3 3
BseYI C'CCAG_C
Cuts at: 0 237 816
Size: 237 579
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 690 816
Size: 337 353 126
Fragments arranged by size:
353 337 126
BsiEI CG_ry'CG
Cuts at: 0 308 731 816
Size: 308 423 85
Fragments arranged by size:
423 308 85
BsiHKAI G_wGCw'C
Cuts at: 0 416 816
Size: 416 400
BsmBI CGTCTCn'nnnn_
Cuts at: 0 291 304 816
Size: 291 13 512
Fragments arranged by size:
512 291 13
Bsp1286I G_dGCh'C
Cuts at: 0 416 489 580 816
Size: 416 73 91 236
Fragments arranged by size:
416 236 91 73
BsrFI r'CCGG_y
Cuts at: 0 221 816
Size: 221 595
BtsI GCAGTG_nn'
Cuts at: 0 516 572 816
Size: 516 56 244
Fragments arranged by size:
516 244 56
DrdI GACnn_nn'nnGTC
Cuts at: 0 96 816
Size: 96 720
EaeI y'GGCC_r
Cuts at: 0 305 728 816
Size: 305 423 88
Fragments arranged by size:
423 305 88
EagI C'GGCC_G
Cuts at: 0 305 728 816
Size: 305 423 88
Fragments arranged by size:
423 305 88
EarI CTCTTCn'nnn_
Cuts at: 0 32 113 559 816
Size: 32 81 446 257
Fragments arranged by size:
446 257 81 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 526 816
Size: 526 290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 816
Size: 364 452
EcoO109I rG'GnC_Cy
Cuts at: 0 537 816
Size: 537 279
HaeII r_GCGC'y
Cuts at: 0 110 267 299 553 590 596 816
Size: 110 157 32 254 37 6 220
Fragments arranged by size:
254 220 157 110 37 32 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 816
Size: 607 32 177
Fragments arranged by size:
607 177 32
KasI G'GCGC_C
Cuts at: 0 263 295 586 816
Size: 263 32 291 230
Fragments arranged by size:
291 263 230 32
MslI CAynn'nnrTG
Cuts at: 0 779 816
Size: 779 37
MspA1I CmG'CkG
Cuts at: 0 96 611 704 816
Size: 96 515 93 112
Fragments arranged by size:
515 112 96 93
NaeI GCC'GGC
Cuts at: 0 223 816
Size: 223 593
NarI GG'CG_CC
Cuts at: 0 264 296 587 816
Size: 264 32 291 229
Fragments arranged by size:
291 264 229 32
NgoMIV G'CCGG_C
Cuts at: 0 221 816
Size: 221 595
NspI r_CATG'y
Cuts at: 0 375 816
Size: 375 441
PmlI CAC'GTG
Cuts at: 0 638 816
Size: 638 178
PvuII CAG'CTG
Cuts at: 0 704 816
Size: 704 112
SapI GCTCTTCn'nnn_
Cuts at: 0 559 816
Size: 559 257
SfcI C'TryA_G
Cuts at: 0 699 816
Size: 699 117
SfoI GGC'GCC
Cuts at: 0 265 297 588 816
Size: 265 32 291 228
Fragments arranged by size:
291 265 228 32
SmaI CCC'GGG
Cuts at: 0 274 581 816
Size: 274 307 235
Fragments arranged by size:
307 274 235
SphI G_CATG'C
Cuts at: 0 375 816
Size: 375 441
StyI C'CwwG_G
Cuts at: 0 20 77 816
Size: 20 57 739
Fragments arranged by size:
739 57 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 776 808 816
Size: 776 32 8
Fragments arranged by size:
776 32 8
XmaI C'CCGG_G
Cuts at: 0 272 579 816
Size: 272 307 237
Fragments arranged by size:
307 272 237
Enzymes that do cut:
AccI AcuI AflIII AlfI AvaI BanI BanII BbeI
BbsI BbvCI BglI BlpI Bme1580I BmgBI Bpu10I BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BsrFI BtsI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I HaeII Hin4I KasI MslI MspA1I
NaeI NarI NgoMIV NspI PmlI PvuII SapI SfcI
SfoI SmaI SphI StyI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglII BmrI BmtI BplI BpmI BpuEI BsaBI BsaWI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmnI ZraI