Restriction map for
pF1KB9633
(Linear) (Six-base) MAPSORT of: KIBB9633.seq Check: 3499 from: 1 to: 1386
KIBB9633.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AccI GT'mk_AC
Cuts at: 0 282 1386
Size: 282 1104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 1386
Size: 1358 28
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 941 1386
Size: 941 445
AlwNI CAG_nnn'CTG
Cuts at: 0 1381 1386
Size: 1381 5
ApaI G_GGCC'C
Cuts at: 0 892 1386
Size: 892 494
ApoI r'AATT_y
Cuts at: 0 193 747 954 1030 1386
Size: 193 554 207 76 356
Fragments arranged by size:
554 356 207 193 76
AvaI C'yCGr_G
Cuts at: 0 813 1386
Size: 813 573
BanII G_rGCy'C
Cuts at: 0 607 892 1386
Size: 607 285 494
Fragments arranged by size:
607 494 285
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 872 1386
Size: 116 756 514
Fragments arranged by size:
756 514 116
BglII A'GATC_T
Cuts at: 0 265 1386
Size: 265 1121
BlpI GC'TnA_GC
Cuts at: 0 350 1386
Size: 350 1036
Bme1580I G_kGCm'C
Cuts at: 0 892 1110 1386
Size: 892 218 276
Fragments arranged by size:
892 276 218
BmrI ACTGGGnnnn_n'
Cuts at: 0 331 1386
Size: 331 1055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1350 1386
Size: 1350 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 887 1259 1386
Size: 786 101 372 127
Fragments arranged by size:
786 372 127 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 898 1324 1386
Size: 389 509 426 62
Fragments arranged by size:
509 426 389 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1178 1386
Size: 1178 208
BsaHI Gr'CG_yC
Cuts at: 0 40 1386
Size: 40 1346
BsaWI w'CCGG_w
Cuts at: 0 980 1386
Size: 980 406
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 55 784 814 962 992 1386
Size: 25 30 729 30 148 30 394
Fragments arranged by size:
729 394 148 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 1386
Size: 142 1244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1386
Size: 631 755
BsiHKAI G_wGCw'C
Cuts at: 0 497 607 1190 1386
Size: 497 110 583 196
Fragments arranged by size:
583 497 196 110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 47 1386
Size: 47 1339
Bsp1286I G_dGCh'C
Cuts at: 0 497 607 892 1110 1190 1386
Size: 497 110 285 218 80 196
Fragments arranged by size:
497 285 218 196 110 80
BspEI T'CCGG_A
Cuts at: 0 980 1386
Size: 980 406
BsrDI GCAATG_nn'
Cuts at: 0 66 1386
Size: 66 1320
BssSI C'ACGA_G
Cuts at: 0 269 1386
Size: 269 1117
BstBI TT'CG_AA
Cuts at: 0 1088 1386
Size: 1088 298
BstYI r'GATC_y
Cuts at: 0 265 1386
Size: 265 1121
DraI TTT'AAA
Cuts at: 0 344 1386
Size: 344 1042
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 255 1386
Size: 255 1131
EcoICRI GAG'CTC
Cuts at: 0 605 1386
Size: 605 781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 887 1259 1358 1386
Size: 786 101 372 99 28
Fragments arranged by size:
786 372 101 99 28
EcoO109I rG'GnC_Cy
Cuts at: 0 879 888 889 1386
Size: 879 9 1 497
Fragments arranged by size:
879 497 9 1
EcoRI G'AATT_C
Cuts at: 0 193 747 954 1386
Size: 193 554 207 432
Fragments arranged by size:
554 432 207 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 694 726 1057 1089 1386
Size: 25 32 637 32 331 32 297
Fragments arranged by size:
637 331 297 32 32 32 25
HincII GTy'rAC
Cuts at: 0 283 792 1386
Size: 283 509 594
Fragments arranged by size:
594 509 283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 803 1386
Size: 160 643 583
Fragments arranged by size:
643 583 160
MslI CAynn'nnrTG
Cuts at: 0 923 1386
Size: 923 463
MspA1I CmG'CkG
Cuts at: 0 6 436 1386
Size: 6 430 950
Fragments arranged by size:
950 430 6
NdeI CA'TA_TG
Cuts at: 0 399 1386
Size: 399 987
NspI r_CATG'y
Cuts at: 0 179 922 928 1386
Size: 179 743 6 458
Fragments arranged by size:
743 458 179 6
PpuMI rG'GwC_Cy
Cuts at: 0 879 1386
Size: 879 507
PspOMI G'GGCC_C
Cuts at: 0 888 1386
Size: 888 498
PstI C_TGCA'G
Cuts at: 0 625 1326 1386
Size: 625 701 60
Fragments arranged by size:
701 625 60
PvuII CAG'CTG
Cuts at: 0 6 436 1386
Size: 6 430 950
Fragments arranged by size:
950 430 6
SacI G_AGCT'C
Cuts at: 0 607 1386
Size: 607 779
SalI G'TCGA_C
Cuts at: 0 281 1386
Size: 281 1105
SexAI A'CCwGG_T
Cuts at: 0 293 1386
Size: 293 1093
SfcI C'TryA_G
Cuts at: 0 431 621 1322 1386
Size: 431 190 701 64
Fragments arranged by size:
701 431 190 64
SmlI C'TyrA_G
Cuts at: 0 404 913 1339 1386
Size: 404 509 426 47
Fragments arranged by size:
509 426 404 47
SphI G_CATG'C
Cuts at: 0 928 1386
Size: 928 458
StyI C'CwwG_G
Cuts at: 0 884 1386
Size: 884 502
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 719 1386
Size: 719 667
TatI w'GTAC_w
Cuts at: 0 440 1386
Size: 440 946
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 962 994 1386
Size: 962 32 392
Fragments arranged by size:
962 392 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 1386
Size: 60 1326
XmnI GAAnn'nnTTC
Cuts at: 0 197 751 1228 1386
Size: 197 554 477 158
Fragments arranged by size:
554 477 197 158
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI ApoI AvaI BanII
BbsI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BsrDI BssSI BstBI BstYI DraI EciI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII MmeI MslI
MspA1I NdeI NspI PpuMI PspOMI PstI PvuII SacI
SalI SexAI SfcI SmlI SphI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BmgBI BmtI Bpu10I BsaAI BsaBI BseYI
BsiEI BsiWI BsmI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
ZraI