Restriction map for
pF1KB9634
(Linear) (Six-base) MAPSORT of: KIBB9634.seq Check: 7268 from: 1 to: 975
KIBB9634.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
Acc65I G'GTAC_C
Cuts at: 0 876 975
Size: 876 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 558 640 975
Size: 141 417 82 335
Fragments arranged by size:
417 335 141 82
AflIII A'CryG_T
Cuts at: 0 847 975
Size: 847 128
AhdI GACnn_n'nnGTC
Cuts at: 0 947 975
Size: 947 28
AlwNI CAG_nnn'CTG
Cuts at: 0 404 975
Size: 404 571
ApaI G_GGCC'C
Cuts at: 0 548 777 975
Size: 548 229 198
Fragments arranged by size:
548 229 198
AvaI C'yCGr_G
Cuts at: 0 118 726 975
Size: 118 608 249
Fragments arranged by size:
608 249 118
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 731 764 975
Size: 731 33 211
Fragments arranged by size:
731 211 33
BanI G'GyrC_C
Cuts at: 0 699 876 975
Size: 699 177 99
Fragments arranged by size:
699 177 99
BanII G_rGCy'C
Cuts at: 0 548 616 727 777 975
Size: 548 68 111 50 198
Fragments arranged by size:
548 198 111 68 50
BbvCI CC'TCA_GC
Cuts at: 0 237 273 975
Size: 237 36 702
Fragments arranged by size:
702 237 36
BciVI GTATCCnnnnn_n'
Cuts at: 0 19 725 732 975
Size: 19 706 7 243
Fragments arranged by size:
706 243 19 7
Bme1580I G_kGCm'C
Cuts at: 0 548 777 975
Size: 548 229 198
Fragments arranged by size:
548 229 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 349 975
Size: 349 626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 542 975
Size: 360 182 433
Fragments arranged by size:
433 360 182
Bpu10I CC'TnA_GC
Cuts at: 0 237 273 346 975
Size: 237 36 73 629
Fragments arranged by size:
629 237 73 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 975
Size: 739 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 955 975
Size: 955 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 234 237 246 956 975
Size: 225 9 3 9 710 19
Fragments arranged by size:
710 225 19 9 9 3
BseYI C'CCAG_C
Cuts at: 0 151 430 446 487 592 598 651 975
Size: 151 279 16 41 105 6 53 324
Fragments arranged by size:
324 279 151 105 53 41 16 6
Bsp1286I G_dGCh'C
Cuts at: 0 548 616 727 777 975
Size: 548 68 111 50 198
Fragments arranged by size:
548 198 111 68 50
BsrBI CCG'CTC
Cuts at: 0 350 770 975
Size: 350 420 205
Fragments arranged by size:
420 350 205
BsrFI r'CCGG_y
Cuts at: 0 586 975
Size: 586 389
BstAPI GCAn_nnn'nTGC
Cuts at: 0 404 975
Size: 404 571
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 835 975
Size: 108 727 140
Fragments arranged by size:
727 140 108
BtgI C'CryG_G
Cuts at: 0 101 975
Size: 101 874
BtsI GCAGTG_nn'
Cuts at: 0 140 975
Size: 140 835
EaeI y'GGCC_r
Cuts at: 0 110 474 975
Size: 110 364 501
Fragments arranged by size:
501 364 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 360 542 558 640 975
Size: 141 219 182 16 82 335
Fragments arranged by size:
335 219 182 141 82 16
EcoO109I rG'GnC_Cy
Cuts at: 0 46 366 545 773 774 897 941 975
Size: 46 320 179 228 1 123 44 34
Fragments arranged by size:
320 228 179 123 46 44 34 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 885 975
Size: 885 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 933 965 975
Size: 355 32 546 32 10
Fragments arranged by size:
546 355 32 32 10
KpnI G_GTAC'C
Cuts at: 0 880 975
Size: 880 95
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 975
Size: 887 88
MscI TGG'CCA
Cuts at: 0 112 975
Size: 112 863
MslI CAynn'nnrTG
Cuts at: 0 166 836 975
Size: 166 670 139
Fragments arranged by size:
670 166 139
MspA1I CmG'CkG
Cuts at: 0 133 210 528 651 797 975
Size: 133 77 318 123 146 178
Fragments arranged by size:
318 178 146 133 123 77
NcoI C'CATG_G
Cuts at: 0 101 975
Size: 101 874
NspI r_CATG'y
Cuts at: 0 851 975
Size: 851 124
PasI CC'CwG_GG
Cuts at: 0 458 957 975
Size: 458 499 18
Fragments arranged by size:
499 458 18
PciI A'CATG_T
Cuts at: 0 847 975
Size: 847 128
PflMI CCAn_nnn'nTGG
Cuts at: 0 651 975
Size: 651 324
PfoI T'CCnGG_A
Cuts at: 0 42 975
Size: 42 933
PpuMI rG'GwC_Cy
Cuts at: 0 46 941 975
Size: 46 895 34
Fragments arranged by size:
895 46 34
PspOMI G'GGCC_C
Cuts at: 0 544 773 975
Size: 544 229 202
Fragments arranged by size:
544 229 202
PvuII CAG'CTG
Cuts at: 0 210 528 651 975
Size: 210 318 123 324
Fragments arranged by size:
324 318 210 123
SanDI GG'GwC_CC
Cuts at: 0 46 975
Size: 46 929
SmlI C'TyrA_G
Cuts at: 0 718 975
Size: 718 257
StuI AGG'CCT
Cuts at: 0 506 975
Size: 506 469
StyI C'CwwG_G
Cuts at: 0 101 758 975
Size: 101 657 217
Fragments arranged by size:
657 217 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 658 717 975
Size: 658 59 258
Fragments arranged by size:
658 258 59
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 19 105 600 648 975
Size: 19 86 495 48 327
Fragments arranged by size:
495 327 86 48 19
XmnI GAAnn'nnTTC
Cuts at: 0 625 975
Size: 625 350
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AlwNI ApaI AvaI BaeI
BanI BanII BbvCI BciVI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BseRI BseYI Bsp1286I BsrBI BsrFI BstAPI
BstXI BtgI BtsI EaeI Eco57MI EcoO109I FalI Hin4I
KpnI MmeI MscI MslI MspA1I NcoI NspI PasI
PciI PflMI PfoI PpuMI PspOMI PvuII SanDI SmlI
StuI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BbsI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmaI ZraI