Restriction map for
pF1KB9641
(Linear) (Six-base) MAPSORT of: KIBB9641.seq Check: 7808 from: 1 to: 966
KIBB9641.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 966
Size: 277 689
AfeI AGC'GCT
Cuts at: 0 214 688 966
Size: 214 474 278
Fragments arranged by size:
474 278 214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 50 966
Size: 50 916
AlwNI CAG_nnn'CTG
Cuts at: 0 180 966
Size: 180 786
ApaI G_GGCC'C
Cuts at: 0 150 376 966
Size: 150 226 590
Fragments arranged by size:
590 226 150
AvaI C'yCGr_G
Cuts at: 0 75 655 966
Size: 75 580 311
Fragments arranged by size:
580 311 75
BanI G'GyrC_C
Cuts at: 0 65 661 966
Size: 65 596 305
Fragments arranged by size:
596 305 65
BanII G_rGCy'C
Cuts at: 0 82 150 376 544 584 709 966
Size: 82 68 226 168 40 125 257
Fragments arranged by size:
257 226 168 125 82 68 40
Bme1580I G_kGCm'C
Cuts at: 0 68 150 376 664 779 966
Size: 68 82 226 288 115 187
Fragments arranged by size:
288 226 187 115 82 68
BmgBI CAC'GTC
Cuts at: 0 228 966
Size: 228 738
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 681 966
Size: 201 480 285
Fragments arranged by size:
480 285 201
Bpu10I CC'TnA_GC
Cuts at: 0 81 966
Size: 81 885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 966
Size: 460 506
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 966
Size: 907 59
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 652 841 925 966
Size: 649 3 189 84 41
Fragments arranged by size:
649 189 84 41 3
BseYI C'CCAG_C
Cuts at: 0 42 369 376 966
Size: 42 327 7 590
Fragments arranged by size:
590 327 42 7
BsiEI CG_ry'CG
Cuts at: 0 495 647 966
Size: 495 152 319
Fragments arranged by size:
495 319 152
BsiHKAI G_wGCw'C
Cuts at: 0 359 966
Size: 359 607
BsmBI CGTCTCn'nnnn_
Cuts at: 0 163 966
Size: 163 803
Bsp1286I G_dGCh'C
Cuts at: 0 68 82 150 359 376 544 584 664
Size: 68 14 68 209 17 168 40 80
Cuts at: 664 709 779 966
Size: 45 70 187
Fragments arranged by size:
209 187 168 80 70 68 68 45
40 17 14
BsrDI GCAATG_nn'
Cuts at: 0 560 966
Size: 560 406
BsrFI r'CCGG_y
Cuts at: 0 642 966
Size: 642 324
BssSI C'ACGA_G
Cuts at: 0 166 966
Size: 166 800
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 315 966
Size: 195 120 651
Fragments arranged by size:
651 195 120
BstYI r'GATC_y
Cuts at: 0 471 966
Size: 471 495
BtgI C'CryG_G
Cuts at: 0 320 966
Size: 320 646
BtsI GCAGTG_nn'
Cuts at: 0 132 966
Size: 132 834
EaeI y'GGCC_r
Cuts at: 0 317 492 605 644 966
Size: 317 175 113 39 322
Fragments arranged by size:
322 317 175 113 39
EagI C'GGCC_G
Cuts at: 0 492 644 966
Size: 492 152 322
Fragments arranged by size:
492 322 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 499 966
Size: 19 480 467
Fragments arranged by size:
480 467 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 966
Size: 277 689
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 966
Size: 397 569
EcoO109I rG'GnC_Cy
Cuts at: 0 146 147 373 529 966
Size: 146 1 226 156 437
Fragments arranged by size:
437 226 156 146 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 708 966
Size: 267 441 258
Fragments arranged by size:
441 267 258
FspI TGC'GCA
Cuts at: 0 505 966
Size: 505 461
HaeII r_GCGC'y
Cuts at: 0 43 216 427 690 966
Size: 43 173 211 263 276
Fragments arranged by size:
276 263 211 173 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 966
Size: 691 32 243
Fragments arranged by size:
691 243 32
HincII GTy'rAC
Cuts at: 0 522 966
Size: 522 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 966
Size: 734 232
MscI TGG'CCA
Cuts at: 0 607 966
Size: 607 359
MslI CAynn'nnrTG
Cuts at: 0 313 772 966
Size: 313 459 194
Fragments arranged by size:
459 313 194
MspA1I CmG'CkG
Cuts at: 0 17 369 922 966
Size: 17 352 553 44
Fragments arranged by size:
553 352 44 17
NaeI GCC'GGC
Cuts at: 0 644 966
Size: 644 322
NgoMIV G'CCGG_C
Cuts at: 0 642 966
Size: 642 324
NotI GC'GGCC_GC
Cuts at: 0 492 966
Size: 492 474
PasI CC'CwG_GG
Cuts at: 0 60 151 966
Size: 60 91 815
Fragments arranged by size:
815 91 60
PspOMI G'GGCC_C
Cuts at: 0 146 372 966
Size: 146 226 594
Fragments arranged by size:
594 226 146
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 445 477 966
Size: 445 32 489
Fragments arranged by size:
489 445 32
PstI C_TGCA'G
Cuts at: 0 164 941 966
Size: 164 777 25
Fragments arranged by size:
777 164 25
PvuII CAG'CTG
Cuts at: 0 369 966
Size: 369 597
RsrII CG'GwC_CG
Cuts at: 0 6 966
Size: 6 960
ScaI AGT'ACT
Cuts at: 0 463 820 966
Size: 463 357 146
Fragments arranged by size:
463 357 146
SfcI C'TryA_G
Cuts at: 0 160 937 966
Size: 160 777 29
Fragments arranged by size:
777 160 29
SmlI C'TyrA_G
Cuts at: 0 475 966
Size: 475 491
StyI C'CwwG_G
Cuts at: 0 242 966
Size: 242 724
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 23 966
Size: 23 943
TatI w'GTAC_w
Cuts at: 0 461 818 966
Size: 461 357 148
Fragments arranged by size:
461 357 148
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI AvaI BanI BanII
Bme1580I BmgBI BplI Bpu10I BpuEI BsaI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BssSI BstXI
BstYI BtgI BtsI EaeI EagI EciI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NaeI NgoMIV NotI PasI PspOMI PsrI
PstI PvuII RsrII ScaI SfcI SmlI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BpmI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EcoICRI EcoRI EcoRV FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PvuI
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI