Restriction map for
pF1KB9642
(Linear) (Six-base) MAPSORT of: KIBB9642.seq Check: 3510 from: 1 to: 1779
KIBB9642.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1234 1779
Size: 1234 545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1207 1779
Size: 991 216 572
Fragments arranged by size:
991 572 216
AfeI AGC'GCT
Cuts at: 0 565 1779
Size: 565 1214
AleI CACnn'nnGTG
Cuts at: 0 1636 1779
Size: 1636 143
AlwNI CAG_nnn'CTG
Cuts at: 0 480 586 1779
Size: 480 106 1193
Fragments arranged by size:
1193 480 106
ApaI G_GGCC'C
Cuts at: 0 669 1779
Size: 669 1110
BamHI G'GATC_C
Cuts at: 0 1559 1779
Size: 1559 220
BanI G'GyrC_C
Cuts at: 0 798 1719 1779
Size: 798 921 60
Fragments arranged by size:
921 798 60
BanII G_rGCy'C
Cuts at: 0 191 224 669 755 796 1779
Size: 191 33 445 86 41 983
Fragments arranged by size:
983 445 191 86 41 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 372 1779
Size: 372 1407
BbvCI CC'TCA_GC
Cuts at: 0 271 574 823 1779
Size: 271 303 249 956
Fragments arranged by size:
956 303 271 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 334 368 1779
Size: 334 34 1411
Fragments arranged by size:
1411 334 34
BglI GCCn_nnn'nGGC
Cuts at: 0 39 1215 1779
Size: 39 1176 564
Fragments arranged by size:
1176 564 39
BlpI GC'TnA_GC
Cuts at: 0 886 1779
Size: 886 893
Bme1580I G_kGCm'C
Cuts at: 0 669 803 1220 1779
Size: 669 134 417 559
Fragments arranged by size:
669 559 417 134
BmtI G_CTAG'C
Cuts at: 0 538 1779
Size: 538 1241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1431 1779
Size: 1431 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 172 185 208 241 457 502 580
Size: 92 80 13 23 33 216 45 78
Cuts at: 580 718 742 889 1022 1196 1328 1426 1779
Size: 138 24 147 133 174 132 98 353
Fragments arranged by size:
353 216 174 147 138 133 132 98
92 80 78 45 33 24 23 13
Bpu10I CC'TnA_GC
Cuts at: 0 247 271 574 823 1779
Size: 247 24 303 249 956
Fragments arranged by size:
956 303 249 247 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 673 1077 1779
Size: 316 357 404 702
Fragments arranged by size:
702 404 357 316
BsaI GGTCTCn'nnnn_
Cuts at: 0 1310 1779
Size: 1310 469
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 97 127 1779
Size: 97 30 1652
Fragments arranged by size:
1652 97 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 52 1087 1291 1779
Size: 49 3 1035 204 488
Fragments arranged by size:
1035 488 204 49 3
BseYI C'CCAG_C
Cuts at: 0 116 661 678 873 1320 1362 1372 1468
Size: 116 545 17 195 447 42 10 96
Cuts at: 1468 1779
Size: 311
Fragments arranged by size:
545 447 311 195 116 96 42 17
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1441 1779
Size: 93 1348 338
Fragments arranged by size:
1348 338 93
BsiHKAI G_wGCw'C
Cuts at: 0 134 191 550 796 1779
Size: 134 57 359 246 983
Fragments arranged by size:
983 359 246 134 57
BsmI GAATG_Cn'
Cuts at: 0 239 1779
Size: 239 1540
BsmBI CGTCTCn'nnnn_
Cuts at: 0 610 1779
Size: 610 1169
Bsp1286I G_dGCh'C
Cuts at: 0 134 191 224 550 669 755 796 803
Size: 134 57 33 326 119 86 41 7
Cuts at: 803 1220 1779
Size: 417 559
Fragments arranged by size:
559 417 326 134 119 86 57 41
33 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1234 1779
Size: 1234 545
BsrBI CCG'CTC
Cuts at: 0 48 80 1415 1779
Size: 48 32 1335 364
Fragments arranged by size:
1335 364 48 32
BsrDI GCAATG_nn'
Cuts at: 0 941 1268 1467 1779
Size: 941 327 199 312
Fragments arranged by size:
941 327 312 199
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 1779
Size: 234 1545
BstEII G'GTnAC_C
Cuts at: 0 1050 1753 1779
Size: 1050 703 26
Fragments arranged by size:
1050 703 26
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1605 1779
Size: 1605 174
BstYI r'GATC_y
Cuts at: 0 701 843 1409 1559 1779
Size: 701 142 566 150 220
Fragments arranged by size:
701 566 220 150 142
Bsu36I CC'TnA_GG
Cuts at: 0 337 625 832 1483 1779
Size: 337 288 207 651 296
Fragments arranged by size:
651 337 296 288 207
BtgI C'CryG_G
Cuts at: 0 37 312 960 1598 1779
Size: 37 275 648 638 181
Fragments arranged by size:
648 638 275 181 37
BtsI GCAGTG_nn'
Cuts at: 0 1763 1779
Size: 1763 16
DrdI GACnn_nn'nnGTC
Cuts at: 0 1048 1779
Size: 1048 731
EaeI y'GGCC_r
Cuts at: 0 138 712 770 1595 1779
Size: 138 574 58 825 184
Fragments arranged by size:
825 574 184 138 58
EarI CTCTTCn'nnn_
Cuts at: 0 442 1174 1779
Size: 442 732 605
Fragments arranged by size:
732 605 442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 406 1105 1779
Size: 406 699 674
Fragments arranged by size:
699 674 406
EcoICRI GAG'CTC
Cuts at: 0 189 794 1779
Size: 189 605 985
Fragments arranged by size:
985 605 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 172 185 208 241 457 502 580
Size: 92 80 13 23 33 216 45 78
Cuts at: 580 718 742 889 991 1022 1196 1207 1328
Size: 138 24 147 102 31 174 11 121
Cuts at: 1328 1426 1779
Size: 98 353
Fragments arranged by size:
353 216 174 147 138 121 102 98
92 80 78 45 33 31 24 23
13 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 1341 1779
Size: 256 1085 438
Fragments arranged by size:
1085 438 256
EcoO109I rG'GnC_Cy
Cuts at: 0 305 370 521 665 666 837 1255 1386
Size: 305 65 151 144 1 171 418 131
Cuts at: 1386 1439 1487 1567 1779
Size: 53 48 80 212
Fragments arranged by size:
418 305 212 171 151 144 131 80
65 53 48 1
HaeII r_GCGC'y
Cuts at: 0 567 1779
Size: 567 1212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1332 1364 1370 1402 1729 1761
Size: 97 32 1203 32 6 32 327 32
Cuts at: 1761 1779
Size: 18
Fragments arranged by size:
1203 327 97 32 32 32 32 18
6
MscI TGG'CCA
Cuts at: 0 140 714 772 1597 1779
Size: 140 574 58 825 182
Fragments arranged by size:
825 574 182 140 58
MslI CAynn'nnrTG
Cuts at: 0 1507 1549 1636 1779
Size: 1507 42 87 143
Fragments arranged by size:
1507 143 87 42
MspA1I CmG'CkG
Cuts at: 0 89 661 1779
Size: 89 572 1118
Fragments arranged by size:
1118 572 89
NcoI C'CATG_G
Cuts at: 0 37 312 960 1598 1779
Size: 37 275 648 638 181
Fragments arranged by size:
648 638 275 181 37
NheI G'CTAG_C
Cuts at: 0 534 1779
Size: 534 1245
NspI r_CATG'y
Cuts at: 0 932 1140 1291 1779
Size: 932 208 151 488
Fragments arranged by size:
932 488 208 151
PasI CC'CwG_GG
Cuts at: 0 736 1564 1779
Size: 736 828 215
Fragments arranged by size:
828 736 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 264 480 984 1779
Size: 264 216 504 795
Fragments arranged by size:
795 504 264 216
PfoI T'CCnGG_A
Cuts at: 0 191 257 839 905 983 1336 1779
Size: 191 66 582 66 78 353 443
Fragments arranged by size:
582 443 353 191 78 66 66
PpuMI rG'GwC_Cy
Cuts at: 0 837 1386 1487 1779
Size: 837 549 101 292
Fragments arranged by size:
837 549 292 101
PspOMI G'GGCC_C
Cuts at: 0 665 1779
Size: 665 1114
PstI C_TGCA'G
Cuts at: 0 347 1779
Size: 347 1432
PvuII CAG'CTG
Cuts at: 0 89 661 1779
Size: 89 572 1118
Fragments arranged by size:
1118 572 89
SacI G_AGCT'C
Cuts at: 0 191 796 1779
Size: 191 605 983
Fragments arranged by size:
983 605 191
SapI GCTCTTCn'nnn_
Cuts at: 0 1174 1779
Size: 1174 605
SexAI A'CCwGG_T
Cuts at: 0 500 1779
Size: 500 1279
SfcI C'TryA_G
Cuts at: 0 343 1779
Size: 343 1436
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 39 1779
Size: 39 1740
SmlI C'TyrA_G
Cuts at: 0 295 688 1056 1779
Size: 295 393 368 723
Fragments arranged by size:
723 393 368 295
SpeI A'CTAG_T
Cuts at: 0 615 1779
Size: 615 1164
SphI G_CATG'C
Cuts at: 0 932 1291 1779
Size: 932 359 488
Fragments arranged by size:
932 488 359
StuI AGG'CCT
Cuts at: 0 389 1347 1779
Size: 389 958 432
Fragments arranged by size:
958 432 389
StyI C'CwwG_G
Cuts at: 0 37 312 960 1598 1779
Size: 37 275 648 638 181
Fragments arranged by size:
648 638 275 181 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1394 1426 1779
Size: 1394 32 353
Fragments arranged by size:
1394 353 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 26 958 1129 1779
Size: 26 932 171 650
Fragments arranged by size:
932 650 171 26
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI BamHI BanI
BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I MscI MslI MspA1I NcoI NheI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI
SexAI SfcI SfiI SmlI SpeI SphI StuI StyI
TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BciVI BclI BfrBI BglII BmgBI
BmrI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI
BspHI BsrFI BsrGI BssHII BssSI BstBI BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI