(Linear) (Six-base) MAPSORT of: KIBB9643.seq  Check: 4773  from: 1  to: 1221



KIBB9643.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     244    1044    1221 
   Size:        167      77     800     177

  Fragments arranged by size: 

                 800     177     167      77
 
AflIII A'CryG_T

Cuts at:      0     375    1221 
   Size:        375     846
 
AhdI GACnn_n'nnGTC

Cuts at:      0     455    1221 
   Size:        455     766
 
AlwNI CAG_nnn'CTG

Cuts at:      0     190     522    1221 
   Size:        190     332     699

  Fragments arranged by size: 

                 699     332     190
 
ApoI r'AATT_y

Cuts at:      0     129     550     596    1161    1195    1221 
   Size:        129     421      46     565      34      26

  Fragments arranged by size: 

                 565     421     129      46      34      26
 
AseI AT'TA_AT

Cuts at:      0     197    1221 
   Size:        197    1024
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     372     866    1221 
   Size:        372     494     355

  Fragments arranged by size: 

                 494     372     355
 
BbvCI CC'TCA_GC

Cuts at:      0     707    1221 
   Size:        707     514
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     477    1221 
   Size:        477     744
 
BglII A'GATC_T

Cuts at:      0      18     257    1221 
   Size:         18     239     964

  Fragments arranged by size: 

                 964     239      18
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1122    1221 
   Size:       1122      99
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     164    1221 
   Size:        164    1057
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     954    1221 
   Size:        954     267
 
Bpu10I CC'TnA_GC

Cuts at:      0     407     707    1221 
   Size:        407     300     514

  Fragments arranged by size: 

                 514     407     300
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     884    1221 
   Size:        884     337
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     135     442     607    1221 
   Size:        135     307     165     614

  Fragments arranged by size: 

                 614     307     165     135
 
BsaBI GATnn'nnATC

Cuts at:      0     256    1221 
   Size:        256     965
 
BsaHI Gr'CG_yC

Cuts at:      0     456    1221 
   Size:        456     765
 
BsiHKAI G_wGCw'C

Cuts at:      0     368    1221 
   Size:        368     853
 
Bsp1286I G_dGCh'C

Cuts at:      0     368    1221 
   Size:        368     853
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     198    1221 
   Size:        198    1023
 
BsrDI GCAATG_nn'

Cuts at:      0     701    1221 
   Size:        701     520
 
BssSI C'ACGA_G

Cuts at:      0     356    1221 
   Size:        356     865
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      95     753    1221 
   Size:         95     658     468

  Fragments arranged by size: 

                 658     468      95
 
BstYI r'GATC_y

Cuts at:      0      18     257     344    1221 
   Size:         18     239      87     877

  Fragments arranged by size: 

                 877     239      87      18
 
BtgI C'CryG_G

Cuts at:      0      58    1221 
   Size:         58    1163
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      47      82    1221 
   Size:         47      35    1139

  Fragments arranged by size: 

                1139      47      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     439    1221 
   Size:        439     782
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     244     954    1044    1221 
   Size:        167      77     710      90     177

  Fragments arranged by size: 

                 710     177     167      90      77
 
EcoRI G'AATT_C

Cuts at:      0     129    1221 
   Size:        129    1092
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1066    1221 
   Size:       1066     155
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     441     473     758     790    1221 
   Size:        441      32     285      32     431

  Fragments arranged by size: 

                 441     431     285      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     156    1221 
   Size:        156    1065
 
MspA1I CmG'CkG

Cuts at:      0     333    1221 
   Size:        333     888
 
NdeI CA'TA_TG

Cuts at:      0     642    1221 
   Size:        642     579
 
NspI r_CATG'y

Cuts at:      0      29     170     379    1221 
   Size:         29     141     209     842

  Fragments arranged by size: 

                 842     209     141      29
 
PciI A'CATG_T

Cuts at:      0     375    1221 
   Size:        375     846
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     854    1221 
   Size:        854     367
 
PsiI TTA'TAA

Cuts at:      0     944    1221 
   Size:        944     277
 
PstI C_TGCA'G

Cuts at:      0     209    1221 
   Size:        209    1012
 
PvuII CAG'CTG

Cuts at:      0     333    1221 
   Size:        333     888
 
SbfI CC_TGCA'GG

Cuts at:      0     209    1221 
   Size:        209    1012
 
SfcI C'TryA_G

Cuts at:      0     205     514    1221 
   Size:        205     309     707

  Fragments arranged by size: 

                 707     309     205
 
SmlI C'TyrA_G

Cuts at:      0     899    1221 
   Size:        899     322
 
SpeI A'CTAG_T

Cuts at:      0     496     870    1221 
   Size:        496     374     351

  Fragments arranged by size: 

                 496     374     351
 
StyI C'CwwG_G

Cuts at:      0     488    1221 
   Size:        488     733
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI    AlwNI     ApoI     AseI     BbsI    BbvCI
    BciVI    BglII     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI  BsiHKAI Bsp1286I    BspMI    BsrDI    BssSI   BstAPI
    BstYI     BtgI    CspCI     EarI  Eco57MI    EcoRI     FalI    Hin4I
     MslI   MspA1I     NdeI     NspI     PciI    PflMI     PsiI     PstI
    PvuII     SbfI     SfcI     SmlI     SpeI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI    BsaAI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI