(Linear) (Six-base) MAPSORT of: KIBB9646.seq  Check: 2128  from: 1  to: 612



KIBB9646.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     612 
   Size:        326     286
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      26     484     612 
   Size:         26     458     128

  Fragments arranged by size: 

                 458     128      26
 
AlwNI CAG_nnn'CTG

Cuts at:      0     599     612 
   Size:        599      13
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     393     427     612 
   Size:        393      34     185

  Fragments arranged by size: 

                 393     185      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     277     612 
   Size:        277     335
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     504     612 
   Size:        504     108
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     215     612 
   Size:        215     397
 
BsaWI w'CCGG_w

Cuts at:      0      83     612 
   Size:         83     529
 
BseYI C'CCAG_C

Cuts at:      0     279     612 
   Size:        279     333
 
BsiHKAI G_wGCw'C

Cuts at:      0     533     612 
   Size:        533      79
 
BsmI GAATG_Cn'

Cuts at:      0     196     257     612 
   Size:        196      61     355

  Fragments arranged by size: 

                 355     196      61
 
Bsp1286I G_dGCh'C

Cuts at:      0     533     612 
   Size:        533      79
 
BspEI T'CCGG_A

Cuts at:      0      83     612 
   Size:         83     529
 
BsrBI CCG'CTC

Cuts at:      0      79     612 
   Size:         79     533
 
BsrGI T'GTAC_A

Cuts at:      0     488     612 
   Size:        488     124
 
BstYI r'GATC_y

Cuts at:      0     441     612 
   Size:        441     171
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      56     612 
   Size:         56     556
 
EaeI y'GGCC_r

Cuts at:      0     319     612 
   Size:        319     293
 
EarI CTCTTCn'nnn_

Cuts at:      0      99     612 
   Size:         99     513
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     612 
   Size:        326     286
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     612 
   Size:        376     236
 
MscI TGG'CCA

Cuts at:      0     321     612 
   Size:        321     291
 
MspA1I CmG'CkG

Cuts at:      0      58     279     612 
   Size:         58     221     333

  Fragments arranged by size: 

                 333     221      58
 
NdeI CA'TA_TG

Cuts at:      0       9     612 
   Size:          9     603
 
NruI TCG'CGA

Cuts at:      0     215     612 
   Size:        215     397
 
PfoI T'CCnGG_A

Cuts at:      0     167     329     612 
   Size:        167     162     283

  Fragments arranged by size: 

                 283     167     162
 
PsiI TTA'TAA

Cuts at:      0     380     612 
   Size:        380     232
 
PstI C_TGCA'G

Cuts at:      0     463     612 
   Size:        463     149
 
PvuII CAG'CTG

Cuts at:      0      58     279     612 
   Size:         58     221     333

  Fragments arranged by size: 

                 333     221      58
 
SapI GCTCTTCn'nnn_

Cuts at:      0      99     612 
   Size:         99     513
 
SfcI C'TryA_G

Cuts at:      0     459     591     612 
   Size:        459     132      21

  Fragments arranged by size: 

                 459     132      21
 
SmlI C'TyrA_G

Cuts at:      0     519     612 
   Size:        519      93
 
SspI AAT'ATT

Cuts at:      0      72     612 
   Size:         72     540
 
TatI w'GTAC_w

Cuts at:      0     488     612 
   Size:        488     124
 
Tth111I GACn'n_nGTC

Cuts at:      0     392     612 
   Size:        392     220
 
XbaI T'CTAG_A

Cuts at:      0     237     612 
   Size:        237     375
 
XmnI GAAnn'nnTTC

Cuts at:      0      93     196     612 
   Size:         93     103     416

  Fragments arranged by size: 

                 416     103      93
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     BcgI    BciVI    BpuEI     BsaI    BsaWI
    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI    BsrBI    BsrGI    BstYI
    BtgZI     EaeI     EarI  Eco57MI     MmeI     MscI   MspA1I     NdeI
     NruI     PfoI     PsiI     PstI    PvuII     SapI     SfcI     SmlI
     SspI     TatI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI
    BspHI    BspMI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI     XcmI     XhoI     XmaI     ZraI