Restriction map for
pF1KB9647
(Linear) (Six-base) MAPSORT of: KIBB9647.seq Check: 1828 from: 1 to: 1938
KIBB9647.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 0 1131 1467 1887 1938
Size: 1131 336 420 51
Fragments arranged by size:
1131 420 336 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 836 1088 1676 1938
Size: 668 168 252 588 262
Fragments arranged by size:
668 588 262 252 168
AleI CACnn'nnGTG
Cuts at: 0 665 833 1938
Size: 665 168 1105
Fragments arranged by size:
1105 665 168
ApaLI G'TGCA_C
Cuts at: 0 16 1938
Size: 16 1922
ApoI r'AATT_y
Cuts at: 0 117 221 237 386 580 722 890 1310
Size: 117 104 16 149 194 142 168 420
Cuts at: 1310 1478 1562 1910 1938
Size: 168 84 348 28
Fragments arranged by size:
420 348 194 168 168 149 142 117
104 84 28 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1865 1938
Size: 857 1008 73
Fragments arranged by size:
1008 857 73
Bme1580I G_kGCm'C
Cuts at: 0 20 1556 1938
Size: 20 1536 382
Fragments arranged by size:
1536 382 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 752 920 1172 1256 1508 1760 1844
Size: 335 417 168 252 84 252 252 84
Cuts at: 1844 1938
Size: 94
Fragments arranged by size:
417 335 252 252 252 168 94 84
84
BseYI C'CCAG_C
Cuts at: 0 1549 1938
Size: 1549 389
BsiHKAI G_wGCw'C
Cuts at: 0 20 1938
Size: 20 1918
Bsp1286I G_dGCh'C
Cuts at: 0 20 1556 1938
Size: 20 1536 382
Fragments arranged by size:
1536 382 20
BsrGI T'GTAC_A
Cuts at: 0 1572 1929 1938
Size: 1572 357 9
Fragments arranged by size:
1572 357 9
BstZ17I GTA'TAC
Cuts at: 0 1132 1468 1938
Size: 1132 336 470
Fragments arranged by size:
1132 470 336
DraI TTT'AAA
Cuts at: 0 116 1026 1194 1938
Size: 116 910 168 744
Fragments arranged by size:
910 744 168 116
EarI CTCTTCn'nnn_
Cuts at: 0 644 812 980 1064 1652 1916 1938
Size: 644 168 168 84 588 264 22
Fragments arranged by size:
644 588 264 168 168 84 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 668 752 836 920 1088 1172 1256
Size: 335 333 84 84 84 168 84 84
Cuts at: 1256 1508 1676 1760 1844 1938
Size: 252 168 84 84 94
Fragments arranged by size:
335 333 252 168 168 94 84 84
84 84 84 84 84
EcoRI G'AATT_C
Cuts at: 0 221 237 386 722 890 1310 1910 1938
Size: 221 16 149 336 168 420 600 28
Fragments arranged by size:
600 420 336 221 168 149 28 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1442 1474 1938
Size: 1442 32 464
Fragments arranged by size:
1442 464 32
HindIII A'AGCT_T
Cuts at: 0 683 851 1019 1103 1187 1271 1439 1523
Size: 683 168 168 84 84 84 168 84
Cuts at: 1523 1775 1859 1938
Size: 252 84 79
Fragments arranged by size:
683 252 168 168 168 84 84 84
84 84 79
MslI CAynn'nnrTG
Cuts at: 0 665 833 1938
Size: 665 168 1105
Fragments arranged by size:
1105 665 168
NspI r_CATG'y
Cuts at: 0 195 1853 1938
Size: 195 1658 85
Fragments arranged by size:
1658 195 85
PsiI TTA'TAA
Cuts at: 0 32 689 857 1938
Size: 32 657 168 1081
Fragments arranged by size:
1081 657 168 32
SphI G_CATG'C
Cuts at: 0 195 1938
Size: 195 1743
SspI AAT'ATT
Cuts at: 0 74 676 1938
Size: 74 602 1262
Fragments arranged by size:
1262 602 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1271 1938
Size: 1271 667
TatI w'GTAC_w
Cuts at: 0 1572 1929 1938
Size: 1572 357 9
Fragments arranged by size:
1572 357 9
XmnI GAAnn'nnTTC
Cuts at: 0 157 1938
Size: 157 1781
Enzymes that do cut:
AccI AcuI AleI ApaLI ApoI BbsI Bme1580I BpmI
BseYI BsiHKAI Bsp1286I BsrGI BstZ17I DraI EarI Eco57MI
EcoRI Hin4I HindIII MslI NspI PsiI SphI SspI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI
StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI