Restriction map for
pF1KB9649
(Linear) (Six-base) MAPSORT of: KIBB9649.seq Check: 4704 from: 1 to: 1323
KIBB9649.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1323
Size: 289 1034
AfeI AGC'GCT
Cuts at: 0 256 1323
Size: 256 1067
AflIII A'CryG_T
Cuts at: 0 161 1323
Size: 161 1162
AlwNI CAG_nnn'CTG
Cuts at: 0 765 798 1140 1323
Size: 765 33 342 183
Fragments arranged by size:
765 342 183 33
ApoI r'AATT_y
Cuts at: 0 73 1101 1323
Size: 73 1028 222
Fragments arranged by size:
1028 222 73
AvaI C'yCGr_G
Cuts at: 0 44 621 862 906 1323
Size: 44 577 241 44 417
Fragments arranged by size:
577 417 241 44 44
BanI G'GyrC_C
Cuts at: 0 466 529 910 1323
Size: 466 63 381 413
Fragments arranged by size:
466 413 381 63
BanII G_rGCy'C
Cuts at: 0 412 487 1225 1323
Size: 412 75 738 98
Fragments arranged by size:
738 412 98 75
BbeI G_GCGC'C
Cuts at: 0 533 914 1323
Size: 533 381 409
Fragments arranged by size:
533 409 381
BglI GCCn_nnn'nGGC
Cuts at: 0 520 777 1323
Size: 520 257 546
Fragments arranged by size:
546 520 257
BglII A'GATC_T
Cuts at: 0 233 1323
Size: 233 1090
BlpI GC'TnA_GC
Cuts at: 0 841 1261 1323
Size: 841 420 62
Fragments arranged by size:
841 420 62
Bme1580I G_kGCm'C
Cuts at: 0 827 1323
Size: 827 496
BmrI ACTGGGnnnn_n'
Cuts at: 0 552 1323
Size: 552 771
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 892 1323
Size: 892 431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 1309 1323
Size: 471 838 14
Fragments arranged by size:
838 471 14
Bpu10I CC'TnA_GC
Cuts at: 0 1093 1323
Size: 1093 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 493 1323
Size: 304 189 830
Fragments arranged by size:
830 304 189
BsaAI yAC'GTr
Cuts at: 0 594 1323
Size: 594 729
BsaHI Gr'CG_yC
Cuts at: 0 133 530 911 1070 1323
Size: 133 397 381 159 253
Fragments arranged by size:
397 381 253 159 133
BsaWI w'CCGG_w
Cuts at: 0 213 1323
Size: 213 1110
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 748 1014 1323
Size: 79 669 266 309
Fragments arranged by size:
669 309 266 79
BseYI C'CCAG_C
Cuts at: 0 246 394 597 1140 1323
Size: 246 148 203 543 183
Fragments arranged by size:
543 246 203 183 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1233 1323
Size: 25 1208 90
Fragments arranged by size:
1208 90 25
BsiEI CG_ry'CG
Cuts at: 0 154 428 524 1160 1323
Size: 154 274 96 636 163
Fragments arranged by size:
636 274 163 154 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 795 1323
Size: 795 528
Bsp1286I G_dGCh'C
Cuts at: 0 412 487 827 1225 1323
Size: 412 75 340 398 98
Fragments arranged by size:
412 398 340 98 75
BspEI T'CCGG_A
Cuts at: 0 213 1323
Size: 213 1110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 1323
Size: 47 1276
BsrBI CCG'CTC
Cuts at: 0 309 617 975 1323
Size: 309 308 358 348
Fragments arranged by size:
358 348 309 308
BsrFI r'CCGG_y
Cuts at: 0 353 427 895 1323
Size: 353 74 468 428
Fragments arranged by size:
468 428 353 74
BssHII G'CGCG_C
Cuts at: 0 1115 1323
Size: 1115 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 497 765 1323
Size: 497 268 558
Fragments arranged by size:
558 497 268
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 1323
Size: 246 1077
BstYI r'GATC_y
Cuts at: 0 233 284 1323
Size: 233 51 1039
Fragments arranged by size:
1039 233 51
BtgI C'CryG_G
Cuts at: 0 518 524 1323
Size: 518 6 799
Fragments arranged by size:
799 518 6
EaeI y'GGCC_r
Cuts at: 0 151 329 386 425 521 1157 1323
Size: 151 178 57 39 96 636 166
Fragments arranged by size:
636 178 166 151 96 57 39
EagI C'GGCC_G
Cuts at: 0 151 425 521 1157 1323
Size: 151 274 96 636 166
Fragments arranged by size:
636 274 166 151 96
EarI CTCTTCn'nnn_
Cuts at: 0 412 1323
Size: 412 911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 319 467 868 1323
Size: 28 291 148 401 455
Fragments arranged by size:
455 401 291 148 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 471 1309 1323
Size: 289 182 838 14
Fragments arranged by size:
838 289 182 14
EcoO109I rG'GnC_Cy
Cuts at: 0 513 1323
Size: 513 810
EcoRI G'AATT_C
Cuts at: 0 73 1323
Size: 73 1250
HaeII r_GCGC'y
Cuts at: 0 55 258 401 533 914 1127 1323
Size: 55 203 143 132 381 213 196
Fragments arranged by size:
381 213 203 196 143 132 55
KasI G'GCGC_C
Cuts at: 0 529 910 1323
Size: 529 381 413
Fragments arranged by size:
529 413 381
MluI A'CGCG_T
Cuts at: 0 161 1323
Size: 161 1162
MspA1I CmG'CkG
Cuts at: 0 520 526 708 795 1040 1052 1061 1140
Size: 520 6 182 87 245 12 9 79
Cuts at: 1140 1323
Size: 183
Fragments arranged by size:
520 245 183 182 87 79 12 9
6
NaeI GCC'GGC
Cuts at: 0 429 897 1323
Size: 429 468 426
Fragments arranged by size:
468 429 426
NarI GG'CG_CC
Cuts at: 0 530 911 1323
Size: 530 381 412
Fragments arranged by size:
530 412 381
NgoMIV G'CCGG_C
Cuts at: 0 427 895 1323
Size: 427 468 428
Fragments arranged by size:
468 428 427
NotI GC'GGCC_GC
Cuts at: 0 521 1323
Size: 521 802
NspI r_CATG'y
Cuts at: 0 222 1323
Size: 222 1101
PfoI T'CCnGG_A
Cuts at: 0 296 1323
Size: 296 1027
PstI C_TGCA'G
Cuts at: 0 707 713 770 1323
Size: 707 6 57 553
Fragments arranged by size:
707 553 57 6
PvuII CAG'CTG
Cuts at: 0 708 795 1140 1323
Size: 708 87 345 183
Fragments arranged by size:
708 345 183 87
SacII CC_GC'GG
Cuts at: 0 521 527 1323
Size: 521 6 796
Fragments arranged by size:
796 521 6
SapI GCTCTTCn'nnn_
Cuts at: 0 412 1323
Size: 412 911
SexAI A'CCwGG_T
Cuts at: 0 1307 1323
Size: 1307 16
SfcI C'TryA_G
Cuts at: 0 703 709 766 936 1323
Size: 703 6 57 170 387
Fragments arranged by size:
703 387 170 57 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 520 1323
Size: 520 803
SfoI GGC'GCC
Cuts at: 0 531 912 1323
Size: 531 381 411
Fragments arranged by size:
531 411 381
SmaI CCC'GGG
Cuts at: 0 46 1323
Size: 46 1277
SmlI C'TyrA_G
Cuts at: 0 319 508 1323
Size: 319 189 815
Fragments arranged by size:
815 319 189
StyI C'CwwG_G
Cuts at: 0 479 533 1323
Size: 479 54 790
Fragments arranged by size:
790 479 54
Tth111I GACn'n_nGTC
Cuts at: 0 132 1323
Size: 132 1191
XmaI C'CCGG_G
Cuts at: 0 44 1323
Size: 44 1279
XmnI GAAnn'nnTTC
Cuts at: 0 163 1323
Size: 163 1160
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApoI AvaI BanI BanII
BbeI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstXI BstYI BtgI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI HaeII KasI MluI MspA1I NaeI
NarI NgoMIV NotI NspI PfoI PstI PvuII SacII
SapI SexAI SfcI SfiI SfoI SmaI SmlI StyI
Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BmgBI BmtI BsaI BsaBI BsaXI BsiHKAI BsiWI
BsmI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MmeI MscI MslI
NcoI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XcmI XhoI
ZraI