(Linear) (Six-base) MAPSORT of: KIBB9660.seq  Check: 5005  from: 1  to: 936



KIBB9660.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     426     678     697     762     936 
   Size:        426     252      19      65     174

  Fragments arranged by size: 

                 426     252     174      65      19
 
AflIII A'CryG_T

Cuts at:      0     166     936 
   Size:        166     770
 
AleI CACnn'nnGTG

Cuts at:      0     461     936 
   Size:        461     475
 
ApoI r'AATT_y

Cuts at:      0       6      26      70     936 
   Size:          6      20      44     866

  Fragments arranged by size: 

                 866      44      20       6
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     690     723     936 
   Size:        690      33     213

  Fragments arranged by size: 

                 690     213      33
 
BfrBI ATG'CAT

Cuts at:      0      56     936 
   Size:         56     880
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     278     936 
   Size:        278     658
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     422     506     590     842     926     936 
   Size:        338      84      84      84     252      84      10

  Fragments arranged by size: 

                 338     252      84      84      84      84      10
 
BsmI GAATG_Cn'

Cuts at:      0      58     151     936 
   Size:         58      93     785

  Fragments arranged by size: 

                 785      93      58
 
BsrDI GCAATG_nn'

Cuts at:      0     433     685     936 
   Size:        433     252     251

  Fragments arranged by size: 

                 433     252     251
 
BsrGI T'GTAC_A

Cuts at:      0     163     253     936 
   Size:        163      90     683

  Fragments arranged by size: 

                 683     163      90
 
BssSI C'ACGA_G

Cuts at:      0     721     936 
   Size:        721     215
 
Bsu36I CC'TnA_GG

Cuts at:      0     357     936 
   Size:        357     579
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     422     426     506     590     678     697     762 
   Size:        338      84       4      80      84      88      19      65

Cuts at:    762     842     926     936 
   Size:         80      84      10

  Fragments arranged by size: 

                 338      88      84      84      84      80      80      65
                  19      10       4
 
HindIII A'AGCT_T

Cuts at:      0     605     936 
   Size:        605     331
 
MfeI C'AATT_G

Cuts at:      0     537     762     936 
   Size:        537     225     174

  Fragments arranged by size: 

                 537     225     174
 
MslI CAynn'nnrTG

Cuts at:      0      51     135     165     223     461     551     653     936 
   Size:         51      84      30      58     238      90     102     283

  Fragments arranged by size: 

                 283     238     102      90      84      58      51      30
            
 
NdeI CA'TA_TG

Cuts at:      0     132     936 
   Size:        132     804
 
NsiI A_TGCA'T

Cuts at:      0      58     936 
   Size:         58     878
 
NspI r_CATG'y

Cuts at:      0     170     936 
   Size:        170     766
 
PciI A'CATG_T

Cuts at:      0     166     936 
   Size:        166     770
 
SspI AAT'ATT

Cuts at:      0     279     936 
   Size:        279     657
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     125     936 
   Size:        125     811
 
TatI w'GTAC_w

Cuts at:      0     163     253     936 
   Size:        163      90     683

  Fragments arranged by size: 

                 683     163      90
 
XmnI GAAnn'nnTTC

Cuts at:      0     439     523     691     936 
   Size:        439      84     168     245

  Fragments arranged by size: 

                 439     245     168      84
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     BaeI    BfrBI     BmrI     BpmI
     BsmI    BsrDI    BsrGI    BssSI   Bsu36I  Eco57MI  HindIII     MfeI
     MslI     NdeI     NsiI     NspI     PciI     SspI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI