Restriction map for
pF1KB9665
(Linear) (Six-base) MAPSORT of: KIBB9665.seq Check: 5854 from: 1 to: 1269
KIBB9665.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 254 674 1011 1269
Size: 2 252 420 337 258
Fragments arranged by size:
420 337 258 252 2
AflIII A'CryG_T
Cuts at: 0 1033 1269
Size: 1033 236
AleI CACnn'nnGTG
Cuts at: 0 649 986 1269
Size: 649 337 283
Fragments arranged by size:
649 337 283
ApaLI G'TGCA_C
Cuts at: 0 753 1269
Size: 753 516
ApoI r'AATT_y
Cuts at: 0 110 304 388 724 1269
Size: 110 194 84 336 545
Fragments arranged by size:
545 336 194 110 84
AseI AT'TA_AT
Cuts at: 0 292 1269
Size: 292 977
BamHI G'GATC_C
Cuts at: 0 136 1269
Size: 136 1133
BanII G_rGCy'C
Cuts at: 0 30 198 450 1269
Size: 30 168 252 819
Fragments arranged by size:
819 252 168 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 1166 1269
Size: 1166 103
BbvCI CC'TCA_GC
Cuts at: 0 1070 1269
Size: 1070 199
BclI T'GATC_A
Cuts at: 0 251 1269
Size: 251 1018
BfrBI ATG'CAT
Cuts at: 0 498 582 1012 1269
Size: 498 84 430 257
Fragments arranged by size:
498 430 257 84
BglII A'GATC_T
Cuts at: 0 32 284 368 536 782 1269
Size: 32 252 84 168 246 487
Fragments arranged by size:
487 252 246 168 84 32
BlpI GC'TnA_GC
Cuts at: 0 870 1269
Size: 870 399
Bme1580I G_kGCm'C
Cuts at: 0 757 1269
Size: 757 512
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 827 911 1269
Size: 70 757 84 358
Fragments arranged by size:
757 358 84 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 250 334 502 586 754 1269
Size: 166 84 84 168 84 168 515
Fragments arranged by size:
515 168 168 166 84 84 84
Bpu10I CC'TnA_GC
Cuts at: 0 1070 1269
Size: 1070 199
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1087 1269
Size: 1087 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 867 1119 1269
Size: 783 84 252 150
Fragments arranged by size:
783 252 150 84
BsiHKAI G_wGCw'C
Cuts at: 0 30 198 450 757 871 1269
Size: 30 168 252 307 114 398
Fragments arranged by size:
398 307 252 168 114 30
BsmI GAATG_Cn'
Cuts at: 0 1260 1269
Size: 1260 9
Bsp1286I G_dGCh'C
Cuts at: 0 30 198 450 757 871 1269
Size: 30 168 252 307 114 398
Fragments arranged by size:
398 307 252 168 114 30
BspHI T'CATG_A
Cuts at: 0 1137 1269
Size: 1137 132
BsrDI GCAATG_nn'
Cuts at: 0 665 1269
Size: 665 604
BsrGI T'GTAC_A
Cuts at: 0 1201 1269
Size: 1201 68
BstAPI GCAn_nnn'nTGC
Cuts at: 0 761 845 1269
Size: 761 84 424
Fragments arranged by size:
761 424 84
BstYI r'GATC_y
Cuts at: 0 32 136 284 368 536 782 1269
Size: 32 104 148 84 168 246 487
Fragments arranged by size:
487 246 168 148 104 84 32
BtsI GCAGTG_nn'
Cuts at: 0 846 1269
Size: 846 423
DraIII CAC_nnn'GTG
Cuts at: 0 761 1269
Size: 761 508
EcoICRI GAG'CTC
Cuts at: 0 28 196 448 1269
Size: 28 168 252 821
Fragments arranged by size:
821 252 168 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 166 250 254 334 502 586 674
Size: 2 164 84 4 80 168 84 88
Cuts at: 674 754 1011 1269
Size: 80 257 258
Fragments arranged by size:
258 257 168 164 88 84 84 80
80 4 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 272 1269
Size: 272 997
EcoRI G'AATT_C
Cuts at: 0 110 304 388 724 1269
Size: 110 194 84 336 545
Fragments arranged by size:
545 336 194 110 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 1269
Size: 17 32 1220
Fragments arranged by size:
1220 32 17
HindIII A'AGCT_T
Cuts at: 0 181 518 939 1269
Size: 181 337 421 330
Fragments arranged by size:
421 337 330 181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1269
Size: 475 794
MslI CAynn'nnrTG
Cuts at: 0 77 397 581 649 834 986 1269
Size: 77 320 184 68 185 152 283
Fragments arranged by size:
320 283 185 184 152 77 68
NdeI CA'TA_TG
Cuts at: 0 578 1269
Size: 578 691
NsiI A_TGCA'T
Cuts at: 0 500 584 1014 1269
Size: 500 84 430 255
Fragments arranged by size:
500 430 255 84
NspI r_CATG'y
Cuts at: 0 502 586 1016 1037 1269
Size: 502 84 430 21 232
Fragments arranged by size:
502 430 232 84 21
PciI A'CATG_T
Cuts at: 0 1033 1269
Size: 1033 236
SacI G_AGCT'C
Cuts at: 0 30 198 450 1269
Size: 30 168 252 819
Fragments arranged by size:
819 252 168 30
TatI w'GTAC_w
Cuts at: 0 510 641 1036 1201 1269
Size: 510 131 395 165 68
Fragments arranged by size:
510 395 165 131 68
XmnI GAAnn'nnTTC
Cuts at: 0 15 687 856 1024 1269
Size: 15 672 169 168 245
Fragments arranged by size:
672 245 169 168 15
Enzymes that do cut:
AcuI AflIII AleI ApaLI ApoI AseI BamHI BanII
BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrDI BsrGI BstAPI BstYI BtsI DraIII EcoICRI Eco57MI
EcoNI EcoRI Hin4I HindIII MmeI MslI NdeI NsiI
NspI PciI SacI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AsiSI AvaI
AvrII BaeI BanI BbeI BcgI BciVI BglI BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI