Restriction map for
pF1KB9668
(Linear) (Six-base) MAPSORT of: KIBB9668.seq Check: 7970 from: 1 to: 1494
KIBB9668.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 18 1494
Size: 18 1476
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 663 747 814 1083 1494
Size: 580 83 84 67 269 411
Fragments arranged by size:
580 411 269 84 83 67
AfeI AGC'GCT
Cuts at: 0 1108 1494
Size: 1108 386
AflII C'TTAA_G
Cuts at: 0 1371 1494
Size: 1371 123
AflIII A'CryG_T
Cuts at: 0 401 1494
Size: 401 1093
AleI CACnn'nnGTG
Cuts at: 0 613 974 1494
Size: 613 361 520
Fragments arranged by size:
613 520 361
AlwNI CAG_nnn'CTG
Cuts at: 0 951 1302 1494
Size: 951 351 192
Fragments arranged by size:
951 351 192
ApaI G_GGCC'C
Cuts at: 0 226 257 1494
Size: 226 31 1237
Fragments arranged by size:
1237 226 31
ApaLI G'TGCA_C
Cuts at: 0 379 715 1051 1494
Size: 379 336 336 443
Fragments arranged by size:
443 379 336 336
AvaI C'yCGr_G
Cuts at: 0 409 1140 1494
Size: 409 731 354
Fragments arranged by size:
731 409 354
BanI G'GyrC_C
Cuts at: 0 1144 1494
Size: 1144 350
BanII G_rGCy'C
Cuts at: 0 226 257 355 439 775 943 1027 1370
Size: 226 31 98 84 336 168 84 343
Cuts at: 1370 1494
Size: 124
Fragments arranged by size:
343 336 226 168 124 98 84 84
31
BbeI G_GCGC'C
Cuts at: 0 1148 1494
Size: 1148 346
BbsI GAAGACnn'nnnn_
Cuts at: 0 73 1494
Size: 73 1421
BbvCI CC'TCA_GC
Cuts at: 0 445 1494
Size: 445 1049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 408 442 576 610 828 862 1332 1366
Size: 408 34 134 34 218 34 470 34
Cuts at: 1366 1494
Size: 128
Fragments arranged by size:
470 408 218 134 128 34 34 34
34
Bme1580I G_kGCm'C
Cuts at: 0 226 257 383 409 661 719 1055 1494
Size: 226 31 126 26 252 58 336 439
Fragments arranged by size:
439 336 252 226 126 58 31 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 190 1077 1494
Size: 190 887 417
Fragments arranged by size:
887 417 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 453 721 1494
Size: 125 328 268 773
Fragments arranged by size:
773 328 268 125
Bpu10I CC'TnA_GC
Cuts at: 0 445 1494
Size: 445 1049
BsaAI yAC'GTr
Cuts at: 0 404 1494
Size: 404 1090
BsaHI Gr'CG_yC
Cuts at: 0 1145 1494
Size: 1145 349
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 1494
Size: 319 1175
BseYI C'CCAG_C
Cuts at: 0 110 219 250 1494
Size: 110 109 31 1244
Fragments arranged by size:
1244 110 109 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1288 1494
Size: 637 651 206
Fragments arranged by size:
651 637 206
BsiEI CG_ry'CG
Cuts at: 0 600 733 1494
Size: 600 133 761
Fragments arranged by size:
761 600 133
BsiHKAI G_wGCw'C
Cuts at: 0 383 439 707 719 775 791 943 1043
Size: 383 56 268 12 56 16 152 100
Cuts at: 1043 1055 1370 1463 1494
Size: 12 315 93 31
Fragments arranged by size:
383 315 268 152 100 93 56 56
31 16 12 12
BsmI GAATG_Cn'
Cuts at: 0 1258 1494
Size: 1258 236
Bsp1286I G_dGCh'C
Cuts at: 0 226 257 355 383 409 439 661 707
Size: 226 31 98 28 26 30 222 46
Cuts at: 707 719 775 791 943 1027 1043 1055 1370
Size: 12 56 16 152 84 16 12 315
Cuts at: 1370 1463 1494
Size: 93 31
Fragments arranged by size:
315 226 222 152 98 93 84 56
46 31 31 30 28 26 16 16
12 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 1494
Size: 18 1476
BsrBI CCG'CTC
Cuts at: 0 981 1494
Size: 981 513
BsrDI GCAATG_nn'
Cuts at: 0 66 1494
Size: 66 1428
BsrFI r'CCGG_y
Cuts at: 0 317 707 875 1043 1127 1211 1379 1494
Size: 317 390 168 168 84 84 168 115
Fragments arranged by size:
390 317 168 168 168 115 84 84
BssHII G'CGCG_C
Cuts at: 0 515 725 1494
Size: 515 210 769
Fragments arranged by size:
769 515 210
BssSI C'ACGA_G
Cuts at: 0 75 1494
Size: 75 1419
BstAPI GCAn_nnn'nTGC
Cuts at: 0 497 951 1494
Size: 497 454 543
Fragments arranged by size:
543 497 454
BstXI CCAn_nnnn'nTGG
Cuts at: 0 17 699 1494
Size: 17 682 795
Fragments arranged by size:
795 682 17
BstYI r'GATC_y
Cuts at: 0 797 1494
Size: 797 697
BtgI C'CryG_G
Cuts at: 0 131 1185 1269 1494
Size: 131 1054 84 225
Fragments arranged by size:
1054 225 131 84
BtsI GCAGTG_nn'
Cuts at: 0 1335 1494
Size: 1335 159
EaeI y'GGCC_r
Cuts at: 0 159 597 730 782 814 1005 1494
Size: 159 438 133 52 32 191 489
Fragments arranged by size:
489 438 191 159 133 52 32
EagI C'GGCC_G
Cuts at: 0 597 730 1494
Size: 597 133 764
Fragments arranged by size:
764 597 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 205 1051 1494
Size: 205 846 443
Fragments arranged by size:
846 443 205
EcoICRI GAG'CTC
Cuts at: 0 437 773 941 1368 1494
Size: 437 336 168 427 126
Fragments arranged by size:
437 427 336 168 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 453 580 663 721 747 814 1083
Size: 125 328 127 83 58 26 67 269
Cuts at: 1083 1494
Size: 411
Fragments arranged by size:
411 328 269 127 125 83 67 58
26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 1494
Size: 88 1406
EcoO109I rG'GnC_Cy
Cuts at: 0 51 81 116 205 223 1403 1494
Size: 51 30 35 89 18 1180 91
Fragments arranged by size:
1180 91 89 51 35 30 18
FspI TGC'GCA
Cuts at: 0 1248 1357 1494
Size: 1248 109 137
Fragments arranged by size:
1248 137 109
HaeII r_GCGC'y
Cuts at: 0 1110 1148 1302 1494
Size: 1110 38 154 192
Fragments arranged by size:
1110 192 154 38
KasI G'GCGC_C
Cuts at: 0 1144 1494
Size: 1144 350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 800 1136 1343 1494
Size: 464 336 336 207 151
Fragments arranged by size:
464 336 336 207 151
MslI CAynn'nnrTG
Cuts at: 0 613 974 1494
Size: 613 361 520
Fragments arranged by size:
613 520 361
MspA1I CmG'CkG
Cuts at: 0 165 470 554 572 683 1187 1271 1494
Size: 165 305 84 18 111 504 84 223
Fragments arranged by size:
504 305 223 165 111 84 84 18
NarI GG'CG_CC
Cuts at: 0 1145 1494
Size: 1145 349
NotI GC'GGCC_GC
Cuts at: 0 730 1494
Size: 730 764
PfoI T'CCnGG_A
Cuts at: 0 698 1494
Size: 698 796
PmlI CAC'GTG
Cuts at: 0 404 1494
Size: 404 1090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1306 1338 1494
Size: 1306 32 156
Fragments arranged by size:
1306 156 32
PpuMI rG'GwC_Cy
Cuts at: 0 51 1494
Size: 51 1443
PspOMI G'GGCC_C
Cuts at: 0 222 253 1494
Size: 222 31 1241
Fragments arranged by size:
1241 222 31
PstI C_TGCA'G
Cuts at: 0 29 273 368 956 1342 1494
Size: 29 244 95 588 386 152
Fragments arranged by size:
588 386 244 152 95 29
PvuII CAG'CTG
Cuts at: 0 683 1494
Size: 683 811
SacI G_AGCT'C
Cuts at: 0 439 775 943 1370 1494
Size: 439 336 168 427 124
Fragments arranged by size:
439 427 336 168 124
SacII CC_GC'GG
Cuts at: 0 1188 1272 1494
Size: 1188 84 222
Fragments arranged by size:
1188 222 84
SbfI CC_TGCA'GG
Cuts at: 0 29 1494
Size: 29 1465
SfcI C'TryA_G
Cuts at: 0 25 269 364 952 1338 1494
Size: 25 244 95 588 386 156
Fragments arranged by size:
588 386 244 156 95 25
SfoI GGC'GCC
Cuts at: 0 1146 1494
Size: 1146 348
SgrAI Cr'CCGG_yG
Cuts at: 0 707 875 1043 1127 1379 1494
Size: 707 168 168 84 252 115
Fragments arranged by size:
707 252 168 168 115 84
SmlI C'TyrA_G
Cuts at: 0 1371 1494
Size: 1371 123
StuI AGG'CCT
Cuts at: 0 424 508 676 760 844 1096 1180 1264
Size: 424 84 168 84 84 252 84 84
Cuts at: 1264 1348 1494
Size: 84 146
Fragments arranged by size:
424 252 168 146 84 84 84 84
84 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 123 155 1494
Size: 123 32 1339
Fragments arranged by size:
1339 123 32
XmnI GAAnn'nnTTC
Cuts at: 0 565 901 1432 1494
Size: 565 336 531 62
Fragments arranged by size:
565 531 336 62
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII KasI MmeI MslI MspA1I NarI NotI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII
SbfI SfcI SfoI SgrAI SmlI StuI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI
BspHI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EcoRI EcoRV FalI
FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI
ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI