Restriction map for
pF1KB9672
(Linear) (Six-base) MAPSORT of: KIBB9672.seq Check: 4831 from: 1 to: 1272
KIBB9672.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 562 616 623 1266 1272
Size: 562 54 7 643 6
Fragments arranged by size:
643 562 54 7 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 815 860 1090 1272
Size: 313 502 45 230 182
Fragments arranged by size:
502 313 230 182 45
AflIII A'CryG_T
Cuts at: 0 204 1272
Size: 204 1068
AleI CACnn'nnGTG
Cuts at: 0 1157 1272
Size: 1157 115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 571 1199 1272
Size: 571 628 73
Fragments arranged by size:
628 571 73
AlwNI CAG_nnn'CTG
Cuts at: 0 876 1272
Size: 876 396
ApaLI G'TGCA_C
Cuts at: 0 354 1272
Size: 354 918
ApoI r'AATT_y
Cuts at: 0 87 465 494 647 1272
Size: 87 378 29 153 625
Fragments arranged by size:
625 378 153 87 29
AseI AT'TA_AT
Cuts at: 0 116 1272
Size: 116 1156
AvaI C'yCGr_G
Cuts at: 0 920 1272
Size: 920 352
BanI G'GyrC_C
Cuts at: 0 899 1272
Size: 899 373
BbsI GAAGACnn'nnnn_
Cuts at: 0 425 1087 1272
Size: 425 662 185
Fragments arranged by size:
662 425 185
BciVI GTATCCnnnnn_n'
Cuts at: 0 664 1272
Size: 664 608
BclI T'GATC_A
Cuts at: 0 764 1272
Size: 764 508
BfrBI ATG'CAT
Cuts at: 0 966 1272
Size: 966 306
Bme1580I G_kGCm'C
Cuts at: 0 140 358 910 1272
Size: 140 218 552 362
Fragments arranged by size:
552 362 218 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 898 1272
Size: 290 608 374
Fragments arranged by size:
608 374 290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 1272
Size: 38 30 1204
Fragments arranged by size:
1204 38 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1272
Size: 251 1021
BsiHKAI G_wGCw'C
Cuts at: 0 276 358 1272
Size: 276 82 914
Fragments arranged by size:
914 276 82
BsmI GAATG_Cn'
Cuts at: 0 756 1272
Size: 756 516
Bsp1286I G_dGCh'C
Cuts at: 0 140 276 358 910 1272
Size: 140 136 82 552 362
Fragments arranged by size:
552 362 140 136 82
BsrDI GCAATG_nn'
Cuts at: 0 555 1272
Size: 555 717
BtgI C'CryG_G
Cuts at: 0 1212 1272
Size: 1212 60
BtsI GCAGTG_nn'
Cuts at: 0 1230 1272
Size: 1230 42
DraI TTT'AAA
Cuts at: 0 636 1272
Size: 636 636
DraIII CAC_nnn'GTG
Cuts at: 0 144 345 1272
Size: 144 201 927
Fragments arranged by size:
927 201 144
EaeI y'GGCC_r
Cuts at: 0 1138 1272
Size: 1138 134
EarI CTCTTCn'nnn_
Cuts at: 0 533 726 1272
Size: 533 193 546
Fragments arranged by size:
546 533 193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 313 815 860 898 1090 1272
Size: 290 23 502 45 38 192 182
Fragments arranged by size:
502 290 192 182 45 38 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 569 1272
Size: 569 703
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 38 70 858 890 1272
Size: 38 32 788 32 382
Fragments arranged by size:
788 382 38 32 32
HindIII A'AGCT_T
Cuts at: 0 362 1272
Size: 362 910
MslI CAynn'nnrTG
Cuts at: 0 169 1146 1157 1272
Size: 169 977 11 115
Fragments arranged by size:
977 169 115 11
MspA1I CmG'CkG
Cuts at: 0 682 1272
Size: 682 590
NcoI C'CATG_G
Cuts at: 0 1212 1272
Size: 1212 60
NsiI A_TGCA'T
Cuts at: 0 968 1272
Size: 968 304
NspI r_CATG'y
Cuts at: 0 208 738 1272
Size: 208 530 534
Fragments arranged by size:
534 530 208
PciI A'CATG_T
Cuts at: 0 204 1272
Size: 204 1068
PshAI GACnn'nnGTC
Cuts at: 0 425 1272
Size: 425 847
PspXI vC'TCGA_Gb
Cuts at: 0 920 1272
Size: 920 352
PvuII CAG'CTG
Cuts at: 0 682 1272
Size: 682 590
SfcI C'TryA_G
Cuts at: 0 563 617 773 1272
Size: 563 54 156 499
Fragments arranged by size:
563 499 156 54
SmlI C'TyrA_G
Cuts at: 0 920 1272
Size: 920 352
StuI AGG'CCT
Cuts at: 0 1245 1272
Size: 1245 27
StyI C'CwwG_G
Cuts at: 0 1212 1272
Size: 1212 60
TatI w'GTAC_w
Cuts at: 0 676 1066 1272
Size: 676 390 206
Fragments arranged by size:
676 390 206
XhoI C'TCGA_G
Cuts at: 0 920 1272
Size: 920 352
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI
AseI AvaI BanI BbsI BciVI BclI BfrBI Bme1580I
BpmI BsaXI BsgI BsiHKAI BsmI Bsp1286I BsrDI BtgI
BtsI DraI DraIII EaeI EarI Eco57MI FalI Hin4I
HindIII MslI MspA1I NcoI NsiI NspI PciI PshAI
PspXI PvuII SfcI SmlI StuI StyI TatI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI AscI AsiSI AvrII BaeI BamHI BanII
BbeI BbvCI BcgI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XcmI XmaI XmnI ZraI