(Linear) (Six-base) MAPSORT of: KIBB9673.seq  Check: 4879  from: 1  to: 792



KIBB9673.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     792 
   Size:         88     704
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     243     792 
   Size:        243     549
 
ApoI r'AATT_y

Cuts at:      0     164     792 
   Size:        164     628
 
AseI AT'TA_AT

Cuts at:      0     641     652     792 
   Size:        641      11     140

  Fragments arranged by size: 

                 641     140      11
 
AvrII C'CTAG_G

Cuts at:      0     337     792 
   Size:        337     455
 
BbvCI CC'TCA_GC

Cuts at:      0     618     792 
   Size:        618     174
 
BclI T'GATC_A

Cuts at:      0     237     317     792 
   Size:        237      80     475

  Fragments arranged by size: 

                 475     237      80
 
BfrBI ATG'CAT

Cuts at:      0     727     792 
   Size:        727      65
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     792 
   Size:        572     220
 
Bpu10I CC'TnA_GC

Cuts at:      0     618     792 
   Size:        618     174
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       6      36     240     270     792 
   Size:          6      30     204      30     522

  Fragments arranged by size: 

                 522     204      30      30       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     245     792 
   Size:        245     547
 
BtgI C'CryG_G

Cuts at:      0     436     792 
   Size:        436     356
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     537     792 
   Size:        537     255
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     572     792 
   Size:         88     484     220

  Fragments arranged by size: 

                 484     220      88
 
EcoO109I rG'GnC_Cy

Cuts at:      0     334     792 
   Size:        334     458
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       6      38     206     238     792 
   Size:          6      32     168      32     554

  Fragments arranged by size: 

                 554     168      32      32       6
 
HindIII A'AGCT_T

Cuts at:      0     599     792 
   Size:        599     193
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     792 
   Size:         98     694
 
MslI CAynn'nnrTG

Cuts at:      0     685     792 
   Size:        685     107
 
MspA1I CmG'CkG

Cuts at:      0     438     592     792 
   Size:        438     154     200

  Fragments arranged by size: 

                 438     200     154
 
NsiI A_TGCA'T

Cuts at:      0     729     792 
   Size:        729      63
 
NspI r_CATG'y

Cuts at:      0      36     792 
   Size:         36     756
 
PpuMI rG'GwC_Cy

Cuts at:      0     334     792 
   Size:        334     458
 
PsiI TTA'TAA

Cuts at:      0     106     792 
   Size:        106     686
 
PvuII CAG'CTG

Cuts at:      0     592     792 
   Size:        592     200
 
SacII CC_GC'GG

Cuts at:      0     439     792 
   Size:        439     353
 
ScaI AGT'ACT

Cuts at:      0     474     792 
   Size:        474     318
 
SfcI C'TryA_G

Cuts at:      0     378     792 
   Size:        378     414
 
SphI G_CATG'C

Cuts at:      0      36     792 
   Size:         36     756
 
StyI C'CwwG_G

Cuts at:      0     337     792 
   Size:        337     455
 
TatI w'GTAC_w

Cuts at:      0     472     564     792 
   Size:        472      92     228

  Fragments arranged by size: 

                 472     228      92
 
Tth111I GACn'n_nGTC

Cuts at:      0     507     792 
   Size:        507     285
 
XbaI T'CTAG_A

Cuts at:      0     150     792 
   Size:        150     642
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     AseI    AvrII    BbvCI     BclI    BfrBI
     BpmI   Bpu10I    BsaXI    BseRI     BtgI     DrdI  Eco57MI EcoO109I
    Hin4I  HindIII     MmeI     MslI   MspA1I     NsiI     NspI    PpuMI
     PsiI    PvuII    SacII     ScaI     SfcI     SphI     StyI     TatI
  Tth111I     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TstI     XcmI     XhoI     XmaI
     XmnI     ZraI