(Linear) (Six-base) MAPSORT of: KIBB9677.seq  Check: 1479  from: 1  to: 312



KIBB9677.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     312 
   Size:        142     170
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106     109     121     124     312 
   Size:        106       3      12       3     188

  Fragments arranged by size: 

                 188     106      12       3       3
 
BsrDI GCAATG_nn'

Cuts at:      0     204     312 
   Size:        204     108
 
Bsu36I CC'TnA_GG

Cuts at:      0      74     312 
   Size:         74     238
 
DraI TTT'AAA

Cuts at:      0     168     312 
   Size:        168     144
 
EarI CTCTTCn'nnn_

Cuts at:      0      56      83     312 
   Size:         56      27     229

  Fragments arranged by size: 

                 229      56      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     312 
   Size:        142     170
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     138     170     312 
   Size:        138      32     142

  Fragments arranged by size: 

                 142     138      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     312 
   Size:        253      59
 
PfoI T'CCnGG_A

Cuts at:      0     158     312 
   Size:        158     154
 
SapI GCTCTTCn'nnn_

Cuts at:      0      56     312 
   Size:         56     256
 

 Enzymes that do cut:

     BpmI    BseRI    BsrDI   Bsu36I     DraI     EarI  Eco57MI    Hin4I
     MmeI     PfoI     SapI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI