(Linear) (Six-base) MAPSORT of: KIBB9681.seq  Check: 7895  from: 1  to: 420



KIBB9681.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AflIII A'CryG_T

Cuts at:      0     155     173     420 
   Size:        155      18     247

  Fragments arranged by size: 

                 247     155      18
 
ApoI r'AATT_y

Cuts at:      0     322     420 
   Size:        322      98
 
BseYI C'CCAG_C

Cuts at:      0      73     420 
   Size:         73     347
 
BspHI T'CATG_A

Cuts at:      0     258     420 
   Size:        258     162
 
BstEII G'GTnAC_C

Cuts at:      0      31     420 
   Size:         31     389
 
BtgI C'CryG_G

Cuts at:      0     230     420 
   Size:        230     190
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     309     420 
   Size:        309     111
 
HindIII A'AGCT_T

Cuts at:      0       9     420 
   Size:          9     411
 
MspA1I CmG'CkG

Cuts at:      0      73     232     420 
   Size:         73     159     188

  Fragments arranged by size: 

                 188     159      73
 
NdeI CA'TA_TG

Cuts at:      0     239     420 
   Size:        239     181
 
NspI r_CATG'y

Cuts at:      0     159     177     420 
   Size:        159      18     243

  Fragments arranged by size: 

                 243     159      18
 
PciI A'CATG_T

Cuts at:      0     155     173     420 
   Size:        155      18     247

  Fragments arranged by size: 

                 247     155      18
 
PshAI GACnn'nnGTC

Cuts at:      0     101     420 
   Size:        101     319
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     160     192     420 
   Size:        160      32     228

  Fragments arranged by size: 

                 228     160      32
 
PvuII CAG'CTG

Cuts at:      0      73     420 
   Size:         73     347
 
SacII CC_GC'GG

Cuts at:      0     233     420 
   Size:        233     187
 
TatI w'GTAC_w

Cuts at:      0     176     420 
   Size:        176     244
 
XmnI GAAnn'nnTTC

Cuts at:      0     115     420 
   Size:        115     305
 

 Enzymes that do cut:

   AflIII     ApoI    BseYI    BspHI   BstEII     BtgI     FalI  HindIII
   MspA1I     NdeI     NspI     PciI    PshAI     PsrI    PvuII    SacII
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI