Restriction map for
pF1KB9682
(Linear) (Six-base) MAPSORT of: KIBB9682.seq Check: 801 from: 1 to: 447
KIBB9682.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 298 447
Size: 76 222 149
Fragments arranged by size:
222 149 76
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 447
Size: 319 32 96
Fragments arranged by size:
319 96 32
ApaI G_GGCC'C
Cuts at: 0 43 447
Size: 43 404
AscI GG'CGCG_CC
Cuts at: 0 151 447
Size: 151 296
AvaI C'yCGr_G
Cuts at: 0 352 447
Size: 352 95
BanI G'GyrC_C
Cuts at: 0 142 237 447
Size: 142 95 210
Fragments arranged by size:
210 142 95
BanII G_rGCy'C
Cuts at: 0 43 381 447
Size: 43 338 66
Fragments arranged by size:
338 66 43
BbeI G_GCGC'C
Cuts at: 0 146 447
Size: 146 301
BglI GCCn_nnn'nGGC
Cuts at: 0 418 447
Size: 418 29
BlpI GC'TnA_GC
Cuts at: 0 385 447
Size: 385 62
Bme1580I G_kGCm'C
Cuts at: 0 43 242 447
Size: 43 199 205
Fragments arranged by size:
205 199 43
BmgBI CAC'GTC
Cuts at: 0 291 447
Size: 291 156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 447
Size: 345 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 447
Size: 268 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 447
Size: 211 236
BsaBI GATnn'nnATC
Cuts at: 0 435 447
Size: 435 12
BsaHI Gr'CG_yC
Cuts at: 0 143 447
Size: 143 304
BseYI C'CCAG_C
Cuts at: 0 43 447
Size: 43 404
BsiEI CG_ry'CG
Cuts at: 0 422 447
Size: 422 25
Bsp1286I G_dGCh'C
Cuts at: 0 43 242 381 447
Size: 43 199 139 66
Fragments arranged by size:
199 139 66 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 171 447
Size: 171 276
BsrBI CCG'CTC
Cuts at: 0 77 384 447
Size: 77 307 63
Fragments arranged by size:
307 77 63
BsrFI r'CCGG_y
Cuts at: 0 61 369 447
Size: 61 308 78
Fragments arranged by size:
308 78 61
BssHII G'CGCG_C
Cuts at: 0 50 151 447
Size: 50 101 296
Fragments arranged by size:
296 101 50
BstYI r'GATC_y
Cuts at: 0 430 436 447
Size: 430 6 11
Fragments arranged by size:
430 11 6
BtgI C'CryG_G
Cuts at: 0 35 447
Size: 35 412
EaeI y'GGCC_r
Cuts at: 0 119 419 447
Size: 119 300 28
Fragments arranged by size:
300 119 28
EagI C'GGCC_G
Cuts at: 0 419 447
Size: 419 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 268 298 447
Size: 76 192 30 149
Fragments arranged by size:
192 149 76 30
EcoO109I rG'GnC_Cy
Cuts at: 0 40 309 347 447
Size: 40 269 38 100
Fragments arranged by size:
269 100 40 38
HaeII r_GCGC'y
Cuts at: 0 146 413 447
Size: 146 267 34
Fragments arranged by size:
267 146 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447
Size: 415 32
KasI G'GCGC_C
Cuts at: 0 142 447
Size: 142 305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 447
Size: 299 148
MscI TGG'CCA
Cuts at: 0 121 447
Size: 121 326
MspA1I CmG'CkG
Cuts at: 0 37 47 447
Size: 37 10 400
Fragments arranged by size:
400 37 10
NaeI GCC'GGC
Cuts at: 0 63 447
Size: 63 384
NarI GG'CG_CC
Cuts at: 0 143 447
Size: 143 304
NgoMIV G'CCGG_C
Cuts at: 0 61 447
Size: 61 386
PpuMI rG'GwC_Cy
Cuts at: 0 309 347 447
Size: 309 38 100
Fragments arranged by size:
309 100 38
PspOMI G'GGCC_C
Cuts at: 0 39 447
Size: 39 408
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 199 231 447
Size: 199 32 216
Fragments arranged by size:
216 199 32
PvuII CAG'CTG
Cuts at: 0 47 447
Size: 47 400
RsrII CG'GwC_CG
Cuts at: 0 372 447
Size: 372 75
SacII CC_GC'GG
Cuts at: 0 38 447
Size: 38 409
SanDI GG'GwC_CC
Cuts at: 0 347 447
Size: 347 100
SfoI GGC'GCC
Cuts at: 0 144 447
Size: 144 303
SmaI CCC'GGG
Cuts at: 0 354 447
Size: 354 93
SmlI C'TyrA_G
Cuts at: 0 226 447
Size: 226 221
XmaI C'CCGG_G
Cuts at: 0 352 447
Size: 352 95
Enzymes that do cut:
AcuI AloI ApaI AscI AvaI BanI BanII BbeI
BglI BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaBI
BsaHI BseYI BsiEI Bsp1286I BspMI BsrBI BsrFI BssHII
BstYI BtgI EaeI EagI Eco57MI EcoO109I HaeII Hin4I
KasI MmeI MscI MspA1I NaeI NarI NgoMIV PpuMI
PspOMI PsrI PvuII RsrII SacII SanDI SfoI SmaI
SmlI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmrI BmtI Bpu10I BsaI BsaAI
BsaWI BsaXI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MslI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PstI PvuI SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI