(Linear) (Six-base) MAPSORT of: KIBB9684.seq  Check: 456  from: 1  to: 492



KIBB9684.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     196     492 
   Size:         81     115     296

  Fragments arranged by size: 

                 296     115      81
 
AfeI AGC'GCT

Cuts at:      0      94     492 
   Size:         94     398
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323     492 
   Size:        323     169
 
BbvCI CC'TCA_GC

Cuts at:      0     401     492 
   Size:        401      91
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     447     492 
   Size:        229     218      45

  Fragments arranged by size: 

                 229     218      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     401     492 
   Size:        401      91
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     437     492 
   Size:        355      82      55

  Fragments arranged by size: 

                 355      82      55
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253     492 
   Size:        253     239
 
BseYI C'CCAG_C

Cuts at:      0     124     409     446     492 
   Size:        124     285      37      46

  Fragments arranged by size: 

                 285     124      46      37
 
BsiHKAI G_wGCw'C

Cuts at:      0     278     492 
   Size:        278     214
 
Bsp1286I G_dGCh'C

Cuts at:      0     278     492 
   Size:        278     214
 
BspHI T'CATG_A

Cuts at:      0     132     492 
   Size:        132     360
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     342     492 
   Size:        342     150
 
BstYI r'GATC_y

Cuts at:      0     371     492 
   Size:        371     121
 
BtsI GCAGTG_nn'

Cuts at:      0     276     492 
   Size:        276     216
 
EarI CTCTTCn'nnn_

Cuts at:      0     478     490     492 
   Size:        478      12       2

  Fragments arranged by size: 

                 478      12       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     196     229     447     492 
   Size:         81     115      33     218      45

  Fragments arranged by size: 

                 218     115      81      45      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     267     492 
   Size:        267     225
 
HaeII r_GCGC'y

Cuts at:      0      96     492 
   Size:         96     396
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     492 
   Size:        203     289
 
MspA1I CmG'CkG

Cuts at:      0     124     409     492 
   Size:        124     285      83

  Fragments arranged by size: 

                 285     124      83
 
PasI CC'CwG_GG

Cuts at:      0      12     492 
   Size:         12     480
 
PfoI T'CCnGG_A

Cuts at:      0     206     492 
   Size:        206     286
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     323     355     492 
   Size:        323      32     137

  Fragments arranged by size: 

                 323     137      32
 
PvuII CAG'CTG

Cuts at:      0     124     409     492 
   Size:        124     285      83

  Fragments arranged by size: 

                 285     124      83
 
SmlI C'TyrA_G

Cuts at:      0     334     452     492 
   Size:        334     118      40

  Fragments arranged by size: 

                 334     118      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     255     492 
   Size:        255     237
 
TatI w'GTAC_w

Cuts at:      0     110     492 
   Size:        110     382
 

 Enzymes that do cut:

     AcuI     AfeI     BbsI    BbvCI     BpmI   Bpu10I    BpuEI     BsaI
    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI    BstYI     BtsI     EarI
  Eco57MI    EcoNI    HaeII     MmeI   MspA1I     PasI     PfoI     PsrI
    PvuII     SmlI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI