(Linear) (Six-base) MAPSORT of: KIBB9685.seq  Check: 707  from: 1  to: 585



KIBB9685.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     118     585 
   Size:        118     467
 
ApoI r'AATT_y

Cuts at:      0      13      34      78     474     578     585 
   Size:         13      21      44     396     104       7

  Fragments arranged by size: 

                 396     104      44      21      13       7
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     434     467     585 
   Size:        434      33     118

  Fragments arranged by size: 

                 434     118      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     114     585 
   Size:        114     471
 
BclI T'GATC_A

Cuts at:      0     155     585 
   Size:        155     430
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     217     585 
   Size:        217     368
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     187     585 
   Size:         64     123     398

  Fragments arranged by size: 

                 398     123      64
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     585 
   Size:         61     524
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     585 
   Size:         53     532
 
BsrDI GCAATG_nn'

Cuts at:      0      54     585 
   Size:         54     531
 
BtsI GCAGTG_nn'

Cuts at:      0      65     585 
   Size:         65     520
 
EaeI y'GGCC_r

Cuts at:      0      26     585 
   Size:         26     559
 
EarI CTCTTCn'nnn_

Cuts at:      0     190     585 
   Size:        190     395
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     187     585 
   Size:         64     123     398

  Fragments arranged by size: 

                 398     123      64
 
EcoRI G'AATT_C

Cuts at:      0      34     585 
   Size:         34     551
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     147     179     585 
   Size:        104      32      11      32     406

  Fragments arranged by size: 

                 406     104      32      32      11
 
HindIII A'AGCT_T

Cuts at:      0     539     585 
   Size:        539      46
 
MscI TGG'CCA

Cuts at:      0      28     585 
   Size:         28     557
 
NdeI CA'TA_TG

Cuts at:      0     447     585 
   Size:        447     138
 
PshAI GACnn'nnGTC

Cuts at:      0     254     585 
   Size:        254     331
 
PsiI TTA'TAA

Cuts at:      0     389     585 
   Size:        389     196
 
SexAI A'CCwGG_T

Cuts at:      0     116     585 
   Size:        116     469
 
SfcI C'TryA_G

Cuts at:      0     108     585 
   Size:        108     477
 
StyI C'CwwG_G

Cuts at:      0     126     585 
   Size:        126     459
 
TatI w'GTAC_w

Cuts at:      0     372     585 
   Size:        372     213
 

 Enzymes that do cut:

     AhdI     ApoI     BaeI     BbsI     BclI     BmrI     BpmI    BseRI
     BsgI    BsrDI     BtsI     EaeI     EarI  Eco57MI    EcoRI    Hin4I
  HindIII     MscI     NdeI    PshAI     PsiI    SexAI     SfcI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI