Restriction map for
pF1KB9695
(Linear) (Six-base) MAPSORT of: KIBB9695.seq Check: 8280 from: 1 to: 792
KIBB9695.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 558 792
Size: 558 234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 463 490 792
Size: 463 27 302
Fragments arranged by size:
463 302 27
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 792
Size: 478 32 282
Fragments arranged by size:
478 282 32
ApaI G_GGCC'C
Cuts at: 0 209 792
Size: 209 583
AvaI C'yCGr_G
Cuts at: 0 61 211 310 792
Size: 61 150 99 482
Fragments arranged by size:
482 150 99 61
BanI G'GyrC_C
Cuts at: 0 57 133 652 792
Size: 57 76 519 140
Fragments arranged by size:
519 140 76 57
BanII G_rGCy'C
Cuts at: 0 209 792
Size: 209 583
BbeI G_GCGC'C
Cuts at: 0 61 137 792
Size: 61 76 655
Fragments arranged by size:
655 76 61
BbvCI CC'TCA_GC
Cuts at: 0 705 792
Size: 705 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 519 553 792
Size: 519 34 239
Fragments arranged by size:
519 239 34
BglI GCCn_nnn'nGGC
Cuts at: 0 213 792
Size: 213 579
BglII A'GATC_T
Cuts at: 0 575 792
Size: 575 217
Bme1580I G_kGCm'C
Cuts at: 0 101 175 209 792
Size: 101 74 34 583
Fragments arranged by size:
583 101 74 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 792
Size: 505 287
Bpu10I CC'TnA_GC
Cuts at: 0 705 792
Size: 705 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 792
Size: 220 572
BsaI GGTCTCn'nnnn_
Cuts at: 0 127 792
Size: 127 665
BsaHI Gr'CG_yC
Cuts at: 0 58 134 792
Size: 58 76 658
Fragments arranged by size:
658 76 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 379 478 508 771 792
Size: 349 30 99 30 263 21
Fragments arranged by size:
349 263 99 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 693 744 792
Size: 693 51 48
Fragments arranged by size:
693 51 48
BseYI C'CCAG_C
Cuts at: 0 154 433 792
Size: 154 279 359
Fragments arranged by size:
359 279 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 792
Size: 100 692
BsiEI CG_ry'CG
Cuts at: 0 332 771 792
Size: 332 439 21
Fragments arranged by size:
439 332 21
BsiHKAI G_wGCw'C
Cuts at: 0 718 792
Size: 718 74
Bsp1286I G_dGCh'C
Cuts at: 0 101 175 209 718 792
Size: 101 74 34 509 74
Fragments arranged by size:
509 101 74 74 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 558 792
Size: 558 234
BsrBI CCG'CTC
Cuts at: 0 81 177 792
Size: 81 96 615
Fragments arranged by size:
615 96 81
BsrFI r'CCGG_y
Cuts at: 0 655 792
Size: 655 137
BssHII G'CGCG_C
Cuts at: 0 145 792
Size: 145 647
BstAPI GCAn_nnn'nTGC
Cuts at: 0 114 792
Size: 114 678
BstYI r'GATC_y
Cuts at: 0 575 774 792
Size: 575 199 18
Fragments arranged by size:
575 199 18
Bsu36I CC'TnA_GG
Cuts at: 0 756 792
Size: 756 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 680 792
Size: 680 112
BtsI GCAGTG_nn'
Cuts at: 0 396 792
Size: 396 396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 615 650 792
Size: 615 35 142
Fragments arranged by size:
615 142 35
EaeI y'GGCC_r
Cuts at: 0 329 792
Size: 329 463
EagI C'GGCC_G
Cuts at: 0 329 792
Size: 329 463
EarI CTCTTCn'nnn_
Cuts at: 0 88 792
Size: 88 704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 792
Size: 505 287
EcoO109I rG'GnC_Cy
Cuts at: 0 206 792
Size: 206 586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 261 792
Size: 261 531
FspI TGC'GCA
Cuts at: 0 451 693 792
Size: 451 242 99
Fragments arranged by size:
451 242 99
FspAI rTGC'GCAy
Cuts at: 0 451 693 792
Size: 451 242 99
Fragments arranged by size:
451 242 99
HaeII r_GCGC'y
Cuts at: 0 61 137 792
Size: 61 76 655
Fragments arranged by size:
655 76 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 792
Size: 403 32 357
Fragments arranged by size:
403 357 32
KasI G'GCGC_C
Cuts at: 0 57 133 792
Size: 57 76 659
Fragments arranged by size:
659 76 57
MslI CAynn'nnrTG
Cuts at: 0 406 792
Size: 406 386
MspA1I CmG'CkG
Cuts at: 0 198 337 375 429 474 792
Size: 198 139 38 54 45 318
Fragments arranged by size:
318 198 139 54 45 38
NarI GG'CG_CC
Cuts at: 0 58 134 792
Size: 58 76 658
Fragments arranged by size:
658 76 58
NruI TCG'CGA
Cuts at: 0 541 792
Size: 541 251
PasI CC'CwG_GG
Cuts at: 0 282 792
Size: 282 510
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 792
Size: 478 32 282
Fragments arranged by size:
478 282 32
PspOMI G'GGCC_C
Cuts at: 0 205 792
Size: 205 587
PvuII CAG'CTG
Cuts at: 0 198 429 474 792
Size: 198 231 45 318
Fragments arranged by size:
318 231 198 45
SapI GCTCTTCn'nnn_
Cuts at: 0 88 792
Size: 88 704
ScaI AGT'ACT
Cuts at: 0 268 792
Size: 268 524
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 213 792
Size: 213 579
SfoI GGC'GCC
Cuts at: 0 59 135 792
Size: 59 76 657
Fragments arranged by size:
657 76 59
SmaI CCC'GGG
Cuts at: 0 213 312 792
Size: 213 99 480
Fragments arranged by size:
480 213 99
SmlI C'TyrA_G
Cuts at: 0 235 792
Size: 235 557
TatI w'GTAC_w
Cuts at: 0 266 792
Size: 266 526
XmaI C'CCGG_G
Cuts at: 0 211 310 792
Size: 211 99 482
Fragments arranged by size:
482 211 99
Enzymes that do cut:
AarI AlfI AloI ApaI AvaI BanI BanII BbeI
BbvCI BcgI BglI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI Bsu36I
BtgZI BtsI CspCI EaeI EagI EarI Eco57MI EcoO109I
FalI FspI FspAI HaeII Hin4I KasI MslI MspA1I
NarI NruI PasI PpiI PspOMI PvuII SapI ScaI
SfiI SfoI SmaI SmlI TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstEII BstXI BstZ17I BtgI ClaI DraI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfcI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI ZraI