Restriction map for
pF1KB9697
(Linear) (Six-base) MAPSORT of: KIBB9697.seq Check: 8294 from: 1 to: 810
KIBB9697.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 525 566 810
Size: 525 41 244
Fragments arranged by size:
525 244 41
ApaLI G'TGCA_C
Cuts at: 0 172 810
Size: 172 638
AvaI C'yCGr_G
Cuts at: 0 463 810
Size: 463 347
BanI G'GyrC_C
Cuts at: 0 60 300 589 794 810
Size: 60 240 289 205 16
Fragments arranged by size:
289 240 205 60 16
BanII G_rGCy'C
Cuts at: 0 218 421 464 476 810
Size: 218 203 43 12 334
Fragments arranged by size:
334 218 203 43 12
BbeI G_GCGC'C
Cuts at: 0 798 810
Size: 798 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 59 810
Size: 59 751
BglI GCCn_nnn'nGGC
Cuts at: 0 779 810
Size: 779 31
Bme1580I G_kGCm'C
Cuts at: 0 65 176 592 810
Size: 65 111 416 218
Fragments arranged by size:
416 218 111 65
BmgBI CAC'GTC
Cuts at: 0 282 810
Size: 282 528
BmrI ACTGGGnnnn_n'
Cuts at: 0 159 308 810
Size: 159 149 502
Fragments arranged by size:
502 159 149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 289 340 448 478 810
Size: 202 87 51 108 30 332
Fragments arranged by size:
332 202 108 87 51 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 336 810
Size: 336 474
BsaAI yAC'GTr
Cuts at: 0 171 810
Size: 171 639
BsaHI Gr'CG_yC
Cuts at: 0 795 810
Size: 795 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 810
Size: 316 30 464
Fragments arranged by size:
464 316 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 415 810
Size: 412 3 395
Fragments arranged by size:
412 395 3
BseYI C'CCAG_C
Cuts at: 0 375 380 385 635 768 810
Size: 375 5 5 250 133 42
Fragments arranged by size:
375 250 133 42 5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 810
Size: 247 563
BsiHKAI G_wGCw'C
Cuts at: 0 176 218 810
Size: 176 42 592
Fragments arranged by size:
592 176 42
Bsp1286I G_dGCh'C
Cuts at: 0 65 176 218 421 464 476 592 810
Size: 65 111 42 203 43 12 116 218
Fragments arranged by size:
218 203 116 111 65 43 42 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 542 810
Size: 347 195 268
Fragments arranged by size:
347 268 195
BsrBI CCG'CTC
Cuts at: 0 411 792 810
Size: 411 381 18
Fragments arranged by size:
411 381 18
BssHII G'CGCG_C
Cuts at: 0 685 687 689 810
Size: 685 2 2 121
Fragments arranged by size:
685 121 2 2
BssSI C'ACGA_G
Cuts at: 0 97 810
Size: 97 713
BstYI r'GATC_y
Cuts at: 0 75 506 810
Size: 75 431 304
Fragments arranged by size:
431 304 75
BtgI C'CryG_G
Cuts at: 0 296 774 810
Size: 296 478 36
Fragments arranged by size:
478 296 36
BtsI GCAGTG_nn'
Cuts at: 0 126 810
Size: 126 684
DraIII CAC_nnn'GTG
Cuts at: 0 171 810
Size: 171 639
EaeI y'GGCC_r
Cuts at: 0 321 785 810
Size: 321 464 25
Fragments arranged by size:
464 321 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 463 499 810
Size: 463 36 311
Fragments arranged by size:
463 311 36
EcoICRI GAG'CTC
Cuts at: 0 216 810
Size: 216 594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 289 340 448 478 810
Size: 202 87 51 108 30 332
Fragments arranged by size:
332 202 108 87 51 30
EcoO109I rG'GnC_Cy
Cuts at: 0 780 810
Size: 780 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 810
Size: 609 201
FspI TGC'GCA
Cuts at: 0 697 810
Size: 697 113
HaeII r_GCGC'y
Cuts at: 0 625 798 810
Size: 625 173 12
Fragments arranged by size:
625 173 12
KasI G'GCGC_C
Cuts at: 0 794 810
Size: 794 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 810
Size: 509 301
MscI TGG'CCA
Cuts at: 0 323 810
Size: 323 487
MspA1I CmG'CkG
Cuts at: 0 240 545 635 758 776 810
Size: 240 305 90 123 18 34
Fragments arranged by size:
305 240 123 90 34 18
NarI GG'CG_CC
Cuts at: 0 795 810
Size: 795 15
NcoI C'CATG_G
Cuts at: 0 296 810
Size: 296 514
PflMI CCAn_nnn'nTGG
Cuts at: 0 681 810
Size: 681 129
PmlI CAC'GTG
Cuts at: 0 171 810
Size: 171 639
PstI C_TGCA'G
Cuts at: 0 245 810
Size: 245 565
PvuII CAG'CTG
Cuts at: 0 240 810
Size: 240 570
SacI G_AGCT'C
Cuts at: 0 218 810
Size: 218 592
SacII CC_GC'GG
Cuts at: 0 777 810
Size: 777 33
SexAI A'CCwGG_T
Cuts at: 0 56 326 810
Size: 56 270 484
Fragments arranged by size:
484 270 56
SfcI C'TryA_G
Cuts at: 0 241 810
Size: 241 569
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 779 810
Size: 779 31
SfoI GGC'GCC
Cuts at: 0 796 810
Size: 796 14
SmaI CCC'GGG
Cuts at: 0 465 810
Size: 465 345
SrfI GCCC'GGGC
Cuts at: 0 465 810
Size: 465 345
StyI C'CwwG_G
Cuts at: 0 296 810
Size: 296 514
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 561 810
Size: 561 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 810
Size: 316 32 462
Fragments arranged by size:
462 316 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 316 810
Size: 316 494
XmaI C'CCGG_G
Cuts at: 0 463 810
Size: 463 347
XmnI GAAnn'nnTTC
Cuts at: 0 26 205 810
Size: 26 179 605
Fragments arranged by size:
605 179 26
Enzymes that do cut:
AlwNI ApaLI AvaI BanI BanII BbeI BbsI BglI
Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BssHII
BssSI BstYI BtgI BtsI DraIII EaeI EciI EcoICRI
Eco57MI EcoO109I FalI FspI HaeII KasI MmeI MscI
MspA1I NarI NcoI PflMI PmlI PstI PvuII SacI
SacII SexAI SfcI SfiI SfoI SmaI SrfI StyI
TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I
BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EarI EcoNI EcoRI EcoRV FseI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
StuI SwaI TatI Tth111I XbaI XhoI ZraI