Restriction map for
pF1KB9701
(Linear) (Six-base) MAPSORT of: KIBB9701.seq Check: 9408 from: 1 to: 960
KIBB9701.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 164 960
Size: 164 796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 960
Size: 580 380
AfeI AGC'GCT
Cuts at: 0 649 960
Size: 649 311
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 682 714 960
Size: 682 32 246
Fragments arranged by size:
682 246 32
AlwNI CAG_nnn'CTG
Cuts at: 0 156 960
Size: 156 804
ApaI G_GGCC'C
Cuts at: 0 118 376 960
Size: 118 258 584
Fragments arranged by size:
584 258 118
AvaI C'yCGr_G
Cuts at: 0 84 105 328 346 927 960
Size: 84 21 223 18 581 33
Fragments arranged by size:
581 223 84 33 21 18
BanI G'GyrC_C
Cuts at: 0 319 728 960
Size: 319 409 232
Fragments arranged by size:
409 319 232
BanII G_rGCy'C
Cuts at: 0 13 44 118 206 245 376 550 575
Size: 13 31 74 88 39 131 174 25
Cuts at: 575 904 960
Size: 329 56
Fragments arranged by size:
329 174 131 88 74 56 39 31
25 13
BbeI G_GCGC'C
Cuts at: 0 323 732 960
Size: 323 409 228
Fragments arranged by size:
409 323 228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 723 757 960
Size: 723 34 203
Fragments arranged by size:
723 203 34
BglI GCCn_nnn'nGGC
Cuts at: 0 554 563 960
Size: 554 9 397
Fragments arranged by size:
554 397 9
BglII A'GATC_T
Cuts at: 0 779 960
Size: 779 181
Bme1580I G_kGCm'C
Cuts at: 0 118 376 449 526 960
Size: 118 258 73 77 434
Fragments arranged by size:
434 258 118 77 73
BmgBI CAC'GTC
Cuts at: 0 402 960
Size: 402 558
BmrI ACTGGGnnnn_n'
Cuts at: 0 617 960
Size: 617 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 960
Size: 709 251
Bpu10I CC'TnA_GC
Cuts at: 0 644 960
Size: 644 316
BsaHI Gr'CG_yC
Cuts at: 0 320 494 729 960
Size: 320 174 235 231
Fragments arranged by size:
320 235 231 174
BsaWI w'CCGG_w
Cuts at: 0 794 960
Size: 794 166
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 682 712 960
Size: 682 30 248
Fragments arranged by size:
682 248 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 942 960
Size: 76 866 18
Fragments arranged by size:
866 76 18
BseYI C'CCAG_C
Cuts at: 0 135 149 173 341 375 413 450 552
Size: 135 14 24 168 34 38 37 102
Cuts at: 552 960
Size: 408
Fragments arranged by size:
408 168 135 102 38 37 34 24
14
BsiEI CG_ry'CG
Cuts at: 0 198 337 960
Size: 198 139 623
Fragments arranged by size:
623 198 139
BsiHKAI G_wGCw'C
Cuts at: 0 13 934 960
Size: 13 921 26
Fragments arranged by size:
921 26 13
Bsp1286I G_dGCh'C
Cuts at: 0 13 44 118 206 245 376 449 526
Size: 13 31 74 88 39 131 73 77
Cuts at: 526 550 575 904 934 960
Size: 24 25 329 30 26
Fragments arranged by size:
329 131 88 77 74 73 39 31
30 26 25 24 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 666 960
Size: 164 502 294
Fragments arranged by size:
502 294 164
BsrFI r'CCGG_y
Cuts at: 0 871 960
Size: 871 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 387 960
Size: 387 573
BstYI r'GATC_y
Cuts at: 0 599 779 960
Size: 599 180 181
Fragments arranged by size:
599 181 180
BtgI C'CryG_G
Cuts at: 0 226 284 312 460 575 960
Size: 226 58 28 148 115 385
Fragments arranged by size:
385 226 148 115 58 28
EaeI y'GGCC_r
Cuts at: 0 195 334 536 960
Size: 195 139 202 424
Fragments arranged by size:
424 202 195 139
EagI C'GGCC_G
Cuts at: 0 195 334 960
Size: 195 139 626
Fragments arranged by size:
626 195 139
EcoICRI GAG'CTC
Cuts at: 0 11 960
Size: 11 949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 709 960
Size: 580 129 251
Fragments arranged by size:
580 251 129
EcoO109I rG'GnC_Cy
Cuts at: 0 114 115 372 960
Size: 114 1 257 588
Fragments arranged by size:
588 257 114 1
HaeII r_GCGC'y
Cuts at: 0 142 323 468 515 614 651 732 771
Size: 142 181 145 47 99 37 81 39
Cuts at: 771 960
Size: 189
Fragments arranged by size:
189 181 145 142 99 81 47 39
37
HincII GTy'rAC
Cuts at: 0 618 960
Size: 618 342
HpaI GTT'AAC
Cuts at: 0 618 960
Size: 618 342
KasI G'GCGC_C
Cuts at: 0 319 728 960
Size: 319 409 232
Fragments arranged by size:
409 319 232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 960
Size: 53 907
MslI CAynn'nnrTG
Cuts at: 0 838 960
Size: 838 122
MspA1I CmG'CkG
Cuts at: 0 80 162 228 286 314 454 462 528
Size: 80 82 66 58 28 140 8 66
Cuts at: 528 552 960
Size: 24 408
Fragments arranged by size:
408 140 82 80 66 66 58 28
24 8
NaeI GCC'GGC
Cuts at: 0 873 960
Size: 873 87
NarI GG'CG_CC
Cuts at: 0 320 729 960
Size: 320 409 231
Fragments arranged by size:
409 320 231
NgoMIV G'CCGG_C
Cuts at: 0 871 960
Size: 871 89
NotI GC'GGCC_GC
Cuts at: 0 334 960
Size: 334 626
NspI r_CATG'y
Cuts at: 0 394 960
Size: 394 566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 682 714 960
Size: 31 32 619 32 246
Fragments arranged by size:
619 246 32 32 31
PsiI TTA'TAA
Cuts at: 0 629 960
Size: 629 331
PspOMI G'GGCC_C
Cuts at: 0 114 372 960
Size: 114 258 588
Fragments arranged by size:
588 258 114
PstI C_TGCA'G
Cuts at: 0 167 410 434 960
Size: 167 243 24 526
Fragments arranged by size:
526 243 167 24
RsrII CG'GwC_CG
Cuts at: 0 230 960
Size: 230 730
SacI G_AGCT'C
Cuts at: 0 13 960
Size: 13 947
SacII CC_GC'GG
Cuts at: 0 229 287 315 463 960
Size: 229 58 28 148 497
Fragments arranged by size:
497 229 148 58 28
SfcI C'TryA_G
Cuts at: 0 163 406 430 960
Size: 163 243 24 530
Fragments arranged by size:
530 243 163 24
SfoI GGC'GCC
Cuts at: 0 321 730 960
Size: 321 409 230
Fragments arranged by size:
409 321 230
SmaI CCC'GGG
Cuts at: 0 107 330 960
Size: 107 223 630
Fragments arranged by size:
630 223 107
SphI G_CATG'C
Cuts at: 0 394 960
Size: 394 566
SrfI GCCC'GGGC
Cuts at: 0 107 960
Size: 107 853
StyI C'CwwG_G
Cuts at: 0 368 960
Size: 368 592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 930 960
Size: 930 30
XmaI C'CCGG_G
Cuts at: 0 105 328 960
Size: 105 223 632
Fragments arranged by size:
632 223 105
XmnI GAAnn'nnTTC
Cuts at: 0 697 960
Size: 697 263
Enzymes that do cut:
AarI AcuI AfeI AloI AlwNI ApaI AvaI BanI
BanII BbeI BcgI BglI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstYI BtgI EaeI
EagI EcoICRI Eco57MI EcoO109I HaeII HincII HpaI KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PpiI PsiI PspOMI PstI RsrII SacI SacII SfcI
SfoI SmaI SphI SrfI StyI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BlpI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
KpnI MfeI MluI MscI NcoI NdeI NheI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PspXI PsrI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SspI StuI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI ZraI