Restriction map for
pF1KB9702
(Linear) (Six-base) MAPSORT of: KIBB9702.seq Check: 3089 from: 1 to: 981
KIBB9702.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 239 981
Size: 239 742
Acc65I G'GTAC_C
Cuts at: 0 674 981
Size: 674 307
AfeI AGC'GCT
Cuts at: 0 755 981
Size: 755 226
AlwNI CAG_nnn'CTG
Cuts at: 0 140 367 798 981
Size: 140 227 431 183
Fragments arranged by size:
431 227 183 140
ApaLI G'TGCA_C
Cuts at: 0 332 981
Size: 332 649
AvaI C'yCGr_G
Cuts at: 0 85 238 981
Size: 85 153 743
Fragments arranged by size:
743 153 85
BanI G'GyrC_C
Cuts at: 0 674 882 913 981
Size: 674 208 31 68
Fragments arranged by size:
674 208 68 31
BanII G_rGCy'C
Cuts at: 0 632 981
Size: 632 349
BbeI G_GCGC'C
Cuts at: 0 917 981
Size: 917 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 303 337 612 646 981
Size: 303 34 275 34 335
Fragments arranged by size:
335 303 275 34 34
BclI T'GATC_A
Cuts at: 0 290 981
Size: 290 691
BglII A'GATC_T
Cuts at: 0 488 981
Size: 488 493
Bme1580I G_kGCm'C
Cuts at: 0 336 887 981
Size: 336 551 94
Fragments arranged by size:
551 336 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 981
Size: 255 726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 143 477 483 598 742 853 892
Size: 127 16 334 6 115 144 111 39
Cuts at: 892 981
Size: 89
Fragments arranged by size:
334 144 127 115 111 89 39 16
6
Bpu10I CC'TnA_GC
Cuts at: 0 368 607 981
Size: 368 239 374
Fragments arranged by size:
374 368 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 867 981
Size: 867 114
BsaHI Gr'CG_yC
Cuts at: 0 914 953 981
Size: 914 39 28
Fragments arranged by size:
914 39 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 270 487 981
Size: 270 217 494
Fragments arranged by size:
494 270 217
BseYI C'CCAG_C
Cuts at: 0 847 981
Size: 847 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 981
Size: 634 347
BsiEI CG_ry'CG
Cuts at: 0 483 713 981
Size: 483 230 268
Fragments arranged by size:
483 268 230
BsiHKAI G_wGCw'C
Cuts at: 0 336 632 981
Size: 336 296 349
Fragments arranged by size:
349 336 296
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 981
Size: 172 809
Bsp1286I G_dGCh'C
Cuts at: 0 336 632 887 981
Size: 336 296 255 94
Fragments arranged by size:
336 296 255 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 651 981
Size: 239 412 330
Fragments arranged by size:
412 330 239
BsrBI CCG'CTC
Cuts at: 0 594 981
Size: 594 387
BsrFI r'CCGG_y
Cuts at: 0 357 949 981
Size: 357 592 32
Fragments arranged by size:
592 357 32
BssHII G'CGCG_C
Cuts at: 0 541 851 981
Size: 541 310 130
Fragments arranged by size:
541 310 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 140 981
Size: 140 841
BstYI r'GATC_y
Cuts at: 0 488 981
Size: 488 493
BtgI C'CryG_G
Cuts at: 0 190 398 840 900 981
Size: 190 208 442 60 81
Fragments arranged by size:
442 208 190 81 60
DrdI GACnn_nn'nnGTC
Cuts at: 0 661 981
Size: 661 320
EaeI y'GGCC_r
Cuts at: 0 389 710 947 981
Size: 389 321 237 34
Fragments arranged by size:
389 321 237 34
EagI C'GGCC_G
Cuts at: 0 710 981
Size: 710 271
EarI CTCTTCn'nnn_
Cuts at: 0 150 981
Size: 150 831
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 748 981
Size: 748 233
EcoICRI GAG'CTC
Cuts at: 0 630 981
Size: 630 351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 143 477 483 598 742 853 892
Size: 127 16 334 6 115 144 111 39
Cuts at: 892 981
Size: 89
Fragments arranged by size:
334 144 127 115 111 89 39 16
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 981
Size: 67 914
EcoO109I rG'GnC_Cy
Cuts at: 0 75 185 981
Size: 75 110 796
Fragments arranged by size:
796 110 75
HaeII r_GCGC'y
Cuts at: 0 757 829 917 981
Size: 757 72 88 64
Fragments arranged by size:
757 88 72 64
KasI G'GCGC_C
Cuts at: 0 913 981
Size: 913 68
KpnI G_GTAC'C
Cuts at: 0 678 981
Size: 678 303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 830 981
Size: 701 129 151
Fragments arranged by size:
701 151 129
MspA1I CmG'CkG
Cuts at: 0 350 400 567 576 762 842 936 981
Size: 350 50 167 9 186 80 94 45
Fragments arranged by size:
350 186 167 94 80 50 45 9
NaeI GCC'GGC
Cuts at: 0 359 951 981
Size: 359 592 30
Fragments arranged by size:
592 359 30
NarI GG'CG_CC
Cuts at: 0 914 981
Size: 914 67
NgoMIV G'CCGG_C
Cuts at: 0 357 949 981
Size: 357 592 32
Fragments arranged by size:
592 357 32
PflMI CCAn_nnn'nTGG
Cuts at: 0 196 567 981
Size: 196 371 414
Fragments arranged by size:
414 371 196
PfoI T'CCnGG_A
Cuts at: 0 159 981
Size: 159 822
PpuMI rG'GwC_Cy
Cuts at: 0 75 185 981
Size: 75 110 796
Fragments arranged by size:
796 110 75
PshAI GACnn'nnGTC
Cuts at: 0 712 981
Size: 712 269
PstI C_TGCA'G
Cuts at: 0 236 981
Size: 236 745
PvuII CAG'CTG
Cuts at: 0 567 576 981
Size: 567 9 405
Fragments arranged by size:
567 405 9
SacI G_AGCT'C
Cuts at: 0 632 981
Size: 632 349
SacII CC_GC'GG
Cuts at: 0 401 843 981
Size: 401 442 138
Fragments arranged by size:
442 401 138
SanDI GG'GwC_CC
Cuts at: 0 75 185 981
Size: 75 110 796
Fragments arranged by size:
796 110 75
SapI GCTCTTCn'nnn_
Cuts at: 0 150 981
Size: 150 831
SexAI A'CCwGG_T
Cuts at: 0 670 981
Size: 670 311
SfcI C'TryA_G
Cuts at: 0 232 981
Size: 232 749
SfoI GGC'GCC
Cuts at: 0 915 981
Size: 915 66
SmaI CCC'GGG
Cuts at: 0 240 981
Size: 240 741
SrfI GCCC'GGGC
Cuts at: 0 240 981
Size: 240 741
StyI C'CwwG_G
Cuts at: 0 68 392 981
Size: 68 324 589
Fragments arranged by size:
589 324 68
XmaI C'CCGG_G
Cuts at: 0 238 981
Size: 238 743
Enzymes that do cut:
AarI Acc65I AfeI AlwNI ApaLI AvaI BanI BanII
BbeI BcgI BclI BglII Bme1580I BmrI BpmI Bpu10I
BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI BtgI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII KasI KpnI MmeI MspA1I NaeI NarI
NgoMIV PflMI PfoI PpuMI PshAI PstI PvuII SacI
SacII SanDI SapI SexAI SfcI SfoI SmaI SrfI
StyI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI BglI
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI MfeI MluI MscI
MslI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SbfI ScaI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI