Restriction map for
pF1KB9704
(Linear) (Six-base) MAPSORT of: KIBB9704.seq Check: 1934 from: 1 to: 996
KIBB9704.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 261 996
Size: 261 735
Acc65I G'GTAC_C
Cuts at: 0 671 996
Size: 671 325
AleI CACnn'nnGTG
Cuts at: 0 916 996
Size: 916 80
AlwNI CAG_nnn'CTG
Cuts at: 0 284 996
Size: 284 712
ApaI G_GGCC'C
Cuts at: 0 216 996
Size: 216 780
AvaI C'yCGr_G
Cuts at: 0 355 508 996
Size: 355 153 488
Fragments arranged by size:
488 355 153
BanI G'GyrC_C
Cuts at: 0 671 949 996
Size: 671 278 47
Fragments arranged by size:
671 278 47
BanII G_rGCy'C
Cuts at: 0 216 857 996
Size: 216 641 139
Fragments arranged by size:
641 216 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 631 996
Size: 631 365
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 819 853 996
Size: 819 34 143
Fragments arranged by size:
819 143 34
BglI GCCn_nnn'nGGC
Cuts at: 0 358 554 793 996
Size: 358 196 239 203
Fragments arranged by size:
358 239 203 196
Bme1580I G_kGCm'C
Cuts at: 0 216 996
Size: 216 780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 316 793 957 996
Size: 192 124 477 164 39
Fragments arranged by size:
477 192 164 124 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 996
Size: 475 521
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 58 88 996
Size: 58 30 908
Fragments arranged by size:
908 58 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 996
Size: 313 683
BseYI C'CCAG_C
Cuts at: 0 321 697 996
Size: 321 376 299
Fragments arranged by size:
376 321 299
BsiEI CG_ry'CG
Cuts at: 0 546 783 996
Size: 546 237 213
Fragments arranged by size:
546 237 213
BsmBI CGTCTCn'nnnn_
Cuts at: 0 283 295 996
Size: 283 12 701
Fragments arranged by size:
701 283 12
Bsp1286I G_dGCh'C
Cuts at: 0 216 857 996
Size: 216 641 139
Fragments arranged by size:
641 216 139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 261 996
Size: 261 735
BsrBI CCG'CTC
Cuts at: 0 11 74 318 645 833 996
Size: 11 63 244 327 188 163
Fragments arranged by size:
327 244 188 163 63 11
BsrDI GCAATG_nn'
Cuts at: 0 630 996
Size: 630 366
BsrFI r'CCGG_y
Cuts at: 0 727 767 946 996
Size: 727 40 179 50
Fragments arranged by size:
727 179 50 40
BssHII G'CGCG_C
Cuts at: 0 396 398 400 402 936 996
Size: 396 2 2 2 534 60
Fragments arranged by size:
534 396 60 2 2 2
BssSI C'ACGA_G
Cuts at: 0 382 996
Size: 382 614
BstAPI GCAn_nnn'nTGC
Cuts at: 0 707 996
Size: 707 289
BstEII G'GTnAC_C
Cuts at: 0 966 996
Size: 966 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 823 996
Size: 84 739 173
Fragments arranged by size:
739 173 84
BtgI C'CryG_G
Cuts at: 0 347 407 788 893 996
Size: 347 60 381 105 103
Fragments arranged by size:
381 347 105 103 60
EaeI y'GGCC_r
Cuts at: 0 275 543 794 848 899 996
Size: 275 268 251 54 51 97
Fragments arranged by size:
275 268 251 97 54 51
EagI C'GGCC_G
Cuts at: 0 543 996
Size: 543 453
EarI CTCTTCn'nnn_
Cuts at: 0 218 996
Size: 218 778
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 194 996
Size: 194 802
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 316 793 957 996
Size: 192 124 477 164 39
Fragments arranged by size:
477 192 164 124 39
EcoO109I rG'GnC_Cy
Cuts at: 0 212 213 681 996
Size: 212 1 468 315
Fragments arranged by size:
468 315 212 1
FspI TGC'GCA
Cuts at: 0 580 996
Size: 580 416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 874 906 996
Size: 874 32 90
Fragments arranged by size:
874 90 32
KpnI G_GTAC'C
Cuts at: 0 675 996
Size: 675 321
MscI TGG'CCA
Cuts at: 0 277 996
Size: 277 719
MslI CAynn'nnrTG
Cuts at: 0 916 996
Size: 916 80
MspA1I CmG'CkG
Cuts at: 0 143 243 594 790 996
Size: 143 100 351 196 206
Fragments arranged by size:
351 206 196 143 100
NotI GC'GGCC_GC
Cuts at: 0 543 996
Size: 543 453
NspI r_CATG'y
Cuts at: 0 840 996
Size: 840 156
PpuMI rG'GwC_Cy
Cuts at: 0 681 996
Size: 681 315
PspOMI G'GGCC_C
Cuts at: 0 212 996
Size: 212 784
PspXI vC'TCGA_Gb
Cuts at: 0 508 996
Size: 508 488
PstI C_TGCA'G
Cuts at: 0 497 996
Size: 497 499
PvuII CAG'CTG
Cuts at: 0 243 996
Size: 243 753
SacII CC_GC'GG
Cuts at: 0 791 996
Size: 791 205
SfcI C'TryA_G
Cuts at: 0 493 676 996
Size: 493 183 320
Fragments arranged by size:
493 320 183
SmaI CCC'GGG
Cuts at: 0 357 996
Size: 357 639
SmlI C'TyrA_G
Cuts at: 0 454 508 996
Size: 454 54 488
Fragments arranged by size:
488 454 54
SphI G_CATG'C
Cuts at: 0 840 996
Size: 840 156
StyI C'CwwG_G
Cuts at: 0 473 996
Size: 473 523
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 843 996
Size: 843 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 996
Size: 58 32 906
Fragments arranged by size:
906 58 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 605 996
Size: 605 391
XhoI C'TCGA_G
Cuts at: 0 508 996
Size: 508 488
XmaI C'CCGG_G
Cuts at: 0 355 996
Size: 355 641
Enzymes that do cut:
AarI Acc65I AleI AlwNI ApaI AvaI BanI BanII
BbsI BcgI BglI Bme1580I BpmI BpuEI BsaXI BseRI
BseYI BsiEI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BtgI EaeI EagI
EarI EciI Eco57MI EcoO109I FspI Hin4I KpnI MscI
MslI MspA1I NotI NspI PpuMI PspOMI PspXI PstI
PvuII SacII SfcI SmaI SmlI SphI StyI TaqII
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiHKAI BsiWI BsmI
BspEI BspHI BsrGI BstBI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TatI Tth111I XbaI XmnI ZraI