Restriction map for
pF1KB9707
(Linear) (Six-base) MAPSORT of: KIBB9707.seq Check: 969 from: 1 to: 1026
KIBB9707.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
Acc65I G'GTAC_C
Cuts at: 0 623 1026
Size: 623 403
AflIII A'CryG_T
Cuts at: 0 947 1026
Size: 947 79
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 159 1026
Size: 159 867
AlwNI CAG_nnn'CTG
Cuts at: 0 324 1026
Size: 324 702
ApaI G_GGCC'C
Cuts at: 0 331 352 479 554 562 1026
Size: 331 21 127 75 8 464
Fragments arranged by size:
464 331 127 75 21 8
ApaLI G'TGCA_C
Cuts at: 0 285 1026
Size: 285 741
AvaI C'yCGr_G
Cuts at: 0 345 585 1026
Size: 345 240 441
Fragments arranged by size:
441 345 240
BanI G'GyrC_C
Cuts at: 0 310 376 604 623 1026
Size: 310 66 228 19 403
Fragments arranged by size:
403 310 228 66 19
BanII G_rGCy'C
Cuts at: 0 199 239 331 352 479 554 562 634
Size: 199 40 92 21 127 75 8 72
Cuts at: 634 664 1026
Size: 30 362
Fragments arranged by size:
362 199 127 92 75 72 40 30
21 8
BbeI G_GCGC'C
Cuts at: 0 314 380 1026
Size: 314 66 646
Fragments arranged by size:
646 314 66
BglI GCCn_nnn'nGGC
Cuts at: 0 685 1026
Size: 685 341
BlpI GC'TnA_GC
Cuts at: 0 633 883 1026
Size: 633 250 143
Fragments arranged by size:
633 250 143
Bme1580I G_kGCm'C
Cuts at: 0 289 331 352 479 554 562 974 1026
Size: 289 42 21 127 75 8 412 52
Fragments arranged by size:
412 289 127 75 52 42 21 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 24 201 504 1026
Size: 24 177 303 522
Fragments arranged by size:
522 303 177 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 258 435 653 1026
Size: 258 177 218 373
Fragments arranged by size:
373 258 218 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 763 1026
Size: 29 734 263
Fragments arranged by size:
734 263 29
BsaAI yAC'GTr
Cuts at: 0 950 1026
Size: 950 76
BsaHI Gr'CG_yC
Cuts at: 0 311 377 1026
Size: 311 66 649
Fragments arranged by size:
649 311 66
BsaWI w'CCGG_w
Cuts at: 0 987 1026
Size: 987 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 1026
Size: 181 30 815
Fragments arranged by size:
815 181 30
BseYI C'CCAG_C
Cuts at: 0 458 973 1026
Size: 458 515 53
Fragments arranged by size:
515 458 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1026
Size: 193 833
BsiHKAI G_wGCw'C
Cuts at: 0 289 634 1026
Size: 289 345 392
Fragments arranged by size:
392 345 289
Bsp1286I G_dGCh'C
Cuts at: 0 199 239 289 331 352 479 554 562
Size: 199 40 50 42 21 127 75 8
Cuts at: 562 634 664 974 1026
Size: 72 30 310 52
Fragments arranged by size:
310 199 127 75 72 52 50 42
40 30 21 8
BspEI T'CCGG_A
Cuts at: 0 987 1026
Size: 987 39
BsrBI CCG'CTC
Cuts at: 0 308 932 1026
Size: 308 624 94
Fragments arranged by size:
624 308 94
BsrFI r'CCGG_y
Cuts at: 0 364 1026
Size: 364 662
BssHII G'CGCG_C
Cuts at: 0 429 1026
Size: 429 597
BssSI C'ACGA_G
Cuts at: 0 420 1026
Size: 420 606
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 1026
Size: 828 198
BtgI C'CryG_G
Cuts at: 0 223 256 380 401 554 1026
Size: 223 33 124 21 153 472
Fragments arranged by size:
472 223 153 124 33 21
BtsI GCAGTG_nn'
Cuts at: 0 507 1026
Size: 507 519
DraIII CAC_nnn'GTG
Cuts at: 0 422 1026
Size: 422 604
EarI CTCTTCn'nnn_
Cuts at: 0 257 1026
Size: 257 769
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 247 1019 1026
Size: 247 772 7
Fragments arranged by size:
772 247 7
EcoICRI GAG'CTC
Cuts at: 0 632 1026
Size: 632 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 763 1026
Size: 29 734 263
Fragments arranged by size:
734 263 29
EcoO109I rG'GnC_Cy
Cuts at: 0 328 349 475 715 891 1026
Size: 328 21 126 240 176 135
Fragments arranged by size:
328 240 176 135 126 21
FspI TGC'GCA
Cuts at: 0 925 1026
Size: 925 101
HaeII r_GCGC'y
Cuts at: 0 314 380 1026
Size: 314 66 646
Fragments arranged by size:
646 314 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 181 213 1026
Size: 10 32 139 32 813
Fragments arranged by size:
813 139 32 32 10
HindIII A'AGCT_T
Cuts at: 0 174 1026
Size: 174 852
KasI G'GCGC_C
Cuts at: 0 310 376 1026
Size: 310 66 650
Fragments arranged by size:
650 310 66
KpnI G_GTAC'C
Cuts at: 0 627 1026
Size: 627 399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 763 1026
Size: 650 113 263
Fragments arranged by size:
650 263 113
MslI CAynn'nnrTG
Cuts at: 0 599 1026
Size: 599 427
MspA1I CmG'CkG
Cuts at: 0 186 382 458 556 726 773 810 1026
Size: 186 196 76 98 170 47 37 216
Fragments arranged by size:
216 196 186 170 98 76 47 37
NaeI GCC'GGC
Cuts at: 0 366 1026
Size: 366 660
NarI GG'CG_CC
Cuts at: 0 311 377 1026
Size: 311 66 649
Fragments arranged by size:
649 311 66
NcoI C'CATG_G
Cuts at: 0 223 1026
Size: 223 803
NgoMIV G'CCGG_C
Cuts at: 0 364 1026
Size: 364 662
NspI r_CATG'y
Cuts at: 0 146 598 1026
Size: 146 452 428
Fragments arranged by size:
452 428 146
PasI CC'CwG_GG
Cuts at: 0 324 1026
Size: 324 702
PflMI CCAn_nnn'nTGG
Cuts at: 0 422 542 578 1026
Size: 422 120 36 448
Fragments arranged by size:
448 422 120 36
PfoI T'CCnGG_A
Cuts at: 0 577 740 1026
Size: 577 163 286
Fragments arranged by size:
577 286 163
PpuMI rG'GwC_Cy
Cuts at: 0 715 1026
Size: 715 311
PspOMI G'GGCC_C
Cuts at: 0 327 348 475 550 558 1026
Size: 327 21 127 75 8 468
Fragments arranged by size:
468 327 127 75 21 8
PvuII CAG'CTG
Cuts at: 0 186 458 726 773 810 1026
Size: 186 272 268 47 37 216
Fragments arranged by size:
272 268 216 186 47 37
RsrII CG'GwC_CG
Cuts at: 0 672 1026
Size: 672 354
SacI G_AGCT'C
Cuts at: 0 634 1026
Size: 634 392
SacII CC_GC'GG
Cuts at: 0 383 557 1026
Size: 383 174 469
Fragments arranged by size:
469 383 174
SapI GCTCTTCn'nnn_
Cuts at: 0 257 1026
Size: 257 769
SfoI GGC'GCC
Cuts at: 0 312 378 1026
Size: 312 66 648
Fragments arranged by size:
648 312 66
SmaI CCC'GGG
Cuts at: 0 347 587 1026
Size: 347 240 439
Fragments arranged by size:
439 347 240
SphI G_CATG'C
Cuts at: 0 598 1026
Size: 598 428
SrfI GCCC'GGGC
Cuts at: 0 347 1026
Size: 347 679
StyI C'CwwG_G
Cuts at: 0 111 223 1026
Size: 111 112 803
Fragments arranged by size:
803 112 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 227 1026
Size: 227 799
TatI w'GTAC_w
Cuts at: 0 944 1026
Size: 944 82
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 188 221 1026
Size: 188 33 805
Fragments arranged by size:
805 188 33
XmaI C'CCGG_G
Cuts at: 0 345 585 1026
Size: 345 240 441
Fragments arranged by size:
441 345 240
Enzymes that do cut:
Acc65I AflIII AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BglI BlpI Bme1580I BmrI BplI BpmI
BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I
BspEI BsrBI BsrFI BssHII BssSI BstXI BtgI BtsI
DraIII EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII
Hin4I HindIII KasI KpnI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI
PspOMI PvuII RsrII SacI SacII SapI SfoI SmaI
SphI SrfI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaBI BseRI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EaeI EagI EcoNI EcoRI EcoRV
FalI FseI FspAI HincII HpaI MfeI MluI MscI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmlI SnaBI SpeI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI ZraI