(Linear) (Six-base) MAPSORT of: KIBB9717.seq  Check: 3497  from: 1  to: 1185



KIBB9717.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1160    1185 
   Size:       1160      25
 
AflIII A'CryG_T

Cuts at:      0     244    1065    1185 
   Size:        244     821     120

  Fragments arranged by size: 

                 821     244     120
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     472     791    1185 
   Size:        472     319     394

  Fragments arranged by size: 

                 472     394     319
 
ApoI r'AATT_y

Cuts at:      0     998    1185 
   Size:        998     187
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     110     221    1185 
   Size:        110     111     964

  Fragments arranged by size: 

                 964     111     110
 
BbvCI CC'TCA_GC

Cuts at:      0     764    1185 
   Size:        764     421
 
BclI T'GATC_A

Cuts at:      0     288    1185 
   Size:        288     897
 
BmgBI CAC'GTC

Cuts at:      0     245    1185 
   Size:        245     940
 
BmtI G_CTAG'C

Cuts at:      0     725    1185 
   Size:        725     460
 
Bpu10I CC'TnA_GC

Cuts at:      0     445     764    1185 
   Size:        445     319     421

  Fragments arranged by size: 

                 445     421     319
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     711    1185 
   Size:        711     474
 
BsaAI yAC'GTr

Cuts at:      0     653    1185 
   Size:        653     532
 
BsaBI GATnn'nnATC

Cuts at:      0     293    1185 
   Size:        293     892
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     704    1185 
   Size:        704     481
 
BsiHKAI G_wGCw'C

Cuts at:      0     146    1185 
   Size:        146    1039
 
BsmI GAATG_Cn'

Cuts at:      0     742    1185 
   Size:        742     443
 
Bsp1286I G_dGCh'C

Cuts at:      0     146    1185 
   Size:        146    1039
 
BspHI T'CATG_A

Cuts at:      0    1125    1185 
   Size:       1125      60
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     421    1185 
   Size:        421     764
 
BsrDI GCAATG_nn'

Cuts at:      0     773    1185 
   Size:        773     412
 
BssSI C'ACGA_G

Cuts at:      0     635    1185 
   Size:        635     550
 
BtgI C'CryG_G

Cuts at:      0     147     417    1185 
   Size:        147     270     768

  Fragments arranged by size: 

                 768     270     147
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      42    1185 
   Size:         42    1143
 
DraI TTT'AAA

Cuts at:      0     227     254    1185 
   Size:        227      27     931

  Fragments arranged by size: 

                 931     227      27
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     488    1185 
   Size:        488     697
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1160    1185 
   Size:       1160      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     178    1185 
   Size:        178    1007
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1155    1185 
   Size:       1155      30
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     242    1185 
   Size:        242     943
 
MslI CAynn'nnrTG

Cuts at:      0     182     454     827    1070    1185 
   Size:        182     272     373     243     115

  Fragments arranged by size: 

                 373     272     243     182     115
 
NcoI C'CATG_G

Cuts at:      0     147     417    1185 
   Size:        147     270     768

  Fragments arranged by size: 

                 768     270     147
 
NheI G'CTAG_C

Cuts at:      0     721    1185 
   Size:        721     464
 
NspI r_CATG'y

Cuts at:      0     477    1069    1185 
   Size:        477     592     116

  Fragments arranged by size: 

                 592     477     116
 
PciI A'CATG_T

Cuts at:      0    1065    1185 
   Size:       1065     120
 
PsiI TTA'TAA

Cuts at:      0     437    1185 
   Size:        437     748
 
PstI C_TGCA'G

Cuts at:      0     609    1185 
   Size:        609     576
 
ScaI AGT'ACT

Cuts at:      0     500     892    1185 
   Size:        500     392     293

  Fragments arranged by size: 

                 500     392     293
 
SfcI C'TryA_G

Cuts at:      0     605    1185 
   Size:        605     580
 
SmlI C'TyrA_G

Cuts at:      0     726    1185 
   Size:        726     459
 
SspI AAT'ATT

Cuts at:      0     283     543    1185 
   Size:        283     260     642

  Fragments arranged by size: 

                 642     283     260
 
StyI C'CwwG_G

Cuts at:      0     147     417    1185 
   Size:        147     270     768

  Fragments arranged by size: 

                 768     270     147
 
TatI w'GTAC_w

Cuts at:      0     247     498     627     890    1185 
   Size:        247     251     129     263     295

  Fragments arranged by size: 

                 295     263     251     247     129
 
XmnI GAAnn'nnTTC

Cuts at:      0     107    1185 
   Size:        107    1078
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI     BbsI    BbvCI     BclI    BmgBI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BseRI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrDI    BssSI     BtgI    BtgZI     DraI
     DrdI  Eco57MI     FalI    Hin4I     MmeI     MslI     NcoI     NheI
     NspI     PciI     PsiI     PstI     ScaI     SfcI     SmlI     SspI
     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI     BsaI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI