Restriction map for
pF1KB9720
(Linear) (Six-base) MAPSORT of: KIBB9720.seq Check: 2023 from: 1 to: 1212
KIBB9720.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 147 223 280 852 984 1212
Size: 147 76 57 572 132 228
Fragments arranged by size:
572 228 147 132 76 57
AccI GT'mk_AC
Cuts at: 0 890 1212
Size: 890 322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 1212
Size: 615 597
AfeI AGC'GCT
Cuts at: 0 1045 1212
Size: 1045 167
AflIII A'CryG_T
Cuts at: 0 602 1212
Size: 602 610
AlwNI CAG_nnn'CTG
Cuts at: 0 519 1212
Size: 519 693
AscI GG'CGCG_CC
Cuts at: 0 649 1212
Size: 649 563
AvaI C'yCGr_G
Cuts at: 0 426 438 483 670 1203 1212
Size: 426 12 45 187 533 9
Fragments arranged by size:
533 426 187 45 12 9
BanI G'GyrC_C
Cuts at: 0 109 409 768 1052 1212
Size: 109 300 359 284 160
Fragments arranged by size:
359 300 284 160 109
BanII G_rGCy'C
Cuts at: 0 445 533 725 1212
Size: 445 88 192 487
Fragments arranged by size:
487 445 192 88
BbeI G_GCGC'C
Cuts at: 0 772 1056 1212
Size: 772 284 156
Fragments arranged by size:
772 284 156
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 1141 1212
Size: 201 940 71
Fragments arranged by size:
940 201 71
BbvCI CC'TCA_GC
Cuts at: 0 262 1212
Size: 262 950
BclI T'GATC_A
Cuts at: 0 1154 1212
Size: 1154 58
BglI GCCn_nnn'nGGC
Cuts at: 0 35 440 1212
Size: 35 405 772
Fragments arranged by size:
772 405 35
BlpI GC'TnA_GC
Cuts at: 0 527 1212
Size: 527 685
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 108 1212
Size: 108 1104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 1212
Size: 735 477
Bpu10I CC'TnA_GC
Cuts at: 0 262 709 1212
Size: 262 447 503
Fragments arranged by size:
503 447 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 898 1212
Size: 322 576 314
Fragments arranged by size:
576 322 314
BsaI GGTCTCn'nnnn_
Cuts at: 0 467 1212
Size: 467 745
BsaHI Gr'CG_yC
Cuts at: 0 122 769 941 1053 1138 1212
Size: 122 647 172 112 85 74
Fragments arranged by size:
647 172 122 112 85 74
BsaWI w'CCGG_w
Cuts at: 0 311 1212
Size: 311 901
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 414 1212
Size: 154 260 798
Fragments arranged by size:
798 260 154
BseYI C'CCAG_C
Cuts at: 0 20 588 873 1034 1040 1106 1212
Size: 20 568 285 161 6 66 106
Fragments arranged by size:
568 285 161 106 66 20 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 745 1212
Size: 397 348 467
Fragments arranged by size:
467 397 348
BsiEI CG_ry'CG
Cuts at: 0 83 148 387 548 899 1212
Size: 83 65 239 161 351 313
Fragments arranged by size:
351 313 239 161 83 65
BsiHKAI G_wGCw'C
Cuts at: 0 725 1212
Size: 725 487
BsmBI CGTCTCn'nnnn_
Cuts at: 0 340 1212
Size: 340 872
Bsp1286I G_dGCh'C
Cuts at: 0 445 533 725 1212
Size: 445 88 192 487
Fragments arranged by size:
487 445 192 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 223 280 852 876 984 1212
Size: 147 76 57 572 24 108 228
Fragments arranged by size:
572 228 147 108 76 57 24
BsrBI CCG'CTC
Cuts at: 0 144 191 467 1212
Size: 144 47 276 745
Fragments arranged by size:
745 276 144 47
BsrFI r'CCGG_y
Cuts at: 0 13 275 492 678 787 1212
Size: 13 262 217 186 109 425
Fragments arranged by size:
425 262 217 186 109 13
BssHII G'CGCG_C
Cuts at: 0 75 522 649 819 846 1080 1212
Size: 75 447 127 170 27 234 132
Fragments arranged by size:
447 234 170 132 127 75 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 402 519 1212
Size: 402 117 693
Fragments arranged by size:
693 402 117
BstEII G'GTnAC_C
Cuts at: 0 9 1212
Size: 9 1203
BtgI C'CryG_G
Cuts at: 0 5 413 638 824 1212
Size: 5 408 225 186 388
Fragments arranged by size:
408 388 225 186 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 906 1212
Size: 906 306
BtsI GCAGTG_nn'
Cuts at: 0 464 1212
Size: 464 748
EaeI y'GGCC_r
Cuts at: 0 2 80 145 384 416 545 683 779
Size: 2 78 65 239 32 129 138 96
Cuts at: 779 809 896 1212
Size: 30 87 316
Fragments arranged by size:
316 239 138 129 96 87 78 65
32 30 2
EagI C'GGCC_G
Cuts at: 0 80 145 384 545 896 1212
Size: 80 65 239 161 351 316
Fragments arranged by size:
351 316 239 161 80 65
EarI CTCTTCn'nnn_
Cuts at: 0 484 634 730 934 1212
Size: 484 150 96 204 278
Fragments arranged by size:
484 278 204 150 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 241 485 1212
Size: 241 244 727
Fragments arranged by size:
727 244 241
EcoICRI GAG'CTC
Cuts at: 0 723 1212
Size: 723 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 735 1212
Size: 615 120 477
Fragments arranged by size:
615 477 120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 1212
Size: 363 849
HaeII r_GCGC'y
Cuts at: 0 451 700 754 772 1047 1056 1212
Size: 451 249 54 18 275 9 156
Fragments arranged by size:
451 275 249 156 54 18 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 1212
Size: 82 32 1098
Fragments arranged by size:
1098 82 32
HincII GTy'rAC
Cuts at: 0 891 1212
Size: 891 321
KasI G'GCGC_C
Cuts at: 0 768 1052 1212
Size: 768 284 160
Fragments arranged by size:
768 284 160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1212
Size: 305 907
MscI TGG'CCA
Cuts at: 0 4 1212
Size: 4 1208
MslI CAynn'nnrTG
Cuts at: 0 203 1212
Size: 203 1009
MspA1I CmG'CkG
Cuts at: 0 516 1110 1212
Size: 516 594 102
Fragments arranged by size:
594 516 102
NaeI GCC'GGC
Cuts at: 0 789 1212
Size: 789 423
NarI GG'CG_CC
Cuts at: 0 769 1053 1212
Size: 769 284 159
Fragments arranged by size:
769 284 159
NcoI C'CATG_G
Cuts at: 0 5 1212
Size: 5 1207
NgoMIV G'CCGG_C
Cuts at: 0 787 1212
Size: 787 425
NotI GC'GGCC_GC
Cuts at: 0 384 1212
Size: 384 828
NspI r_CATG'y
Cuts at: 0 1167 1212
Size: 1167 45
PfoI T'CCnGG_A
Cuts at: 0 854 1212
Size: 854 358
PstI C_TGCA'G
Cuts at: 0 158 887 983 1212
Size: 158 729 96 229
Fragments arranged by size:
729 229 158 96
PvuII CAG'CTG
Cuts at: 0 516 1110 1212
Size: 516 594 102
Fragments arranged by size:
594 516 102
SacI G_AGCT'C
Cuts at: 0 725 1212
Size: 725 487
SalI G'TCGA_C
Cuts at: 0 889 1212
Size: 889 323
SapI GCTCTTCn'nnn_
Cuts at: 0 634 730 934 1212
Size: 634 96 204 278
Fragments arranged by size:
634 278 204 96
SbfI CC_TGCA'GG
Cuts at: 0 887 983 1212
Size: 887 96 229
Fragments arranged by size:
887 229 96
ScaI AGT'ACT
Cuts at: 0 328 1212
Size: 328 884
SexAI A'CCwGG_T
Cuts at: 0 689 839 1212
Size: 689 150 373
Fragments arranged by size:
689 373 150
SfcI C'TryA_G
Cuts at: 0 154 883 979 1212
Size: 154 729 96 233
Fragments arranged by size:
729 233 154 96
SfoI GGC'GCC
Cuts at: 0 770 1054 1212
Size: 770 284 158
Fragments arranged by size:
770 284 158
SmaI CCC'GGG
Cuts at: 0 440 485 1212
Size: 440 45 727
Fragments arranged by size:
727 440 45
SmlI C'TyrA_G
Cuts at: 0 337 913 1212
Size: 337 576 299
Fragments arranged by size:
576 337 299
StyI C'CwwG_G
Cuts at: 0 5 1212
Size: 5 1207
TatI w'GTAC_w
Cuts at: 0 326 1212
Size: 326 886
XmaI C'CCGG_G
Cuts at: 0 438 483 1212
Size: 438 45 729
Fragments arranged by size:
729 438 45
XmnI GAAnn'nnTTC
Cuts at: 0 346 1212
Size: 346 866
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI AscI AvaI
BanI BanII BbeI BbsI BbvCI BclI BglI BlpI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstEII BtgI BtgZI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PfoI PstI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SmaI
SmlI StyI TatI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI ApoI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BfrBI BglII Bme1580I BmgBI BmrI
BmtI BsaAI BsaBI BsaXI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstXI BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SanDI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI ZraI